Fam109a (NM_175474) Mouse Tagged ORF Clone

CAT#: MR221504

Fam109a (Myc-DDK-tagged) - Mouse family with sequence similarity 109, member A (Fam109a)

Product Images

Circular map for MR221504

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fam109a
• Synonyms: A230106M15Rik; AU017694; IPIP27A; Ses1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221504 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCAACGAGCGCAGCTTGGCCTTCTACGCAACCTGCGATGCCCCGGTGGACAACGCCGGCTTCC
  TGTACAAGCGTGGTGGCCGTGGGACCGGCTCGCACCGGCGCTGGTTCGTGCTCCGGGGCAATATCCTCTT
  TTACTTCGAGGCAGAAGGCAGCCGCGAGCCGCTGGGCGTCATCCTGCTGGAGGGCTGCACGGTGGAGCTG
  GTGGATGCCCGGGAGGAGTTCGCCTTCGCAGTGCGCTTTGCCGGGGGCCGATCCCGGCCGTACGTGCTGG
  CCGCTGACAGCCAGGCAGCCCTGGAGGGCTGGGTGAAGGCACTGTCCCGGGCCAGCTTCCACTATCTGCG
  CTTGGTGGTGCGGGAGCTGGAACAGCAACTGGCTGCCATGCGCGAGGGAAGCCCCGCCAACGCCTTACCC
  GCCAACCCGAGCCCGGTTTTGACCCAGAGACCCAAGGAGAACGGCTGGGTAGTGTGGAGCACGCTGCCCG
  AGCAGCCCTCGGTAGCCCCTCAGCGGCCGCCGCCACTGCCACCCCGCCGCAGGGCCTCAGCGGCCAACGG
  GCCCTTGGCATCCTTCGCCCAGCTGCATGCGCGATATGGACTAGAGGTGCAGGCCCTTAGGGACCAGTGG
  CGCGGAGGCCAGGCTGGCCTAGCCAGCTTAGAGGTCCCCTGGCATCCTGGTTCTGCTGAGACTCAGACCC
  AGGACCAGCCAGCCCTAAGGGGACATAGTGGGTGTAAGGTATTACATGTATTCCGTTCCGTAGAATGGCC
  TGTTTGCAATCCAGGCTCCCAGGGGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221504 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLNERSLAFYATCDAPVDNAGFLYKRGGRGTGSHRRWFVLRGNILFYFEAEGSREPLGVILLEGCTVEL
  VDAREEFAFAVRFAGGRSRPYVLAADSQAALEGWVKALSRASFHYLRLVVRELEQQLAAMREGSPANALP
  ANPSPVLTQRPKENGWVVWSTLPEQPSVAPQRPPPLPPRRRASAANGPLASFAQLHARYGLEVQALRDQW
  RGGQAGLASLEVPWHPGSAETQTQDQPALRGHSGCKVLHVFRSVEWPVCNPGSQGT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175474
• ORF Size: 801 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175474.4
• RefSeq Size: 2335 bp
• RefSeq ORF: 801 bp
• Locus ID: 231717
• UniProt ID: Q8BH49
• Cytogenetics: 5 F
• MW: 29.2 kDa
• Gene Summary: Plays a role in endocytic trafficking. Required for receptor recycling from endosomes, both to the trans-Golgi network and the plasma membrane.[UniProtKB/Swiss-Prot Function]