Kcnk4 (NM_008431) Mouse Tagged Lenti ORF Clone
CAT#: MR221486L4
- LentiORF®
Lenti ORF clone of Kcnk4 (mGFP-tagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4)
"NM_008431" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Kcnk4 |
Synonyms | MLZ-622; Tex40; TRAAK; TRAAKt |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR221486).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008431 |
ORF Size | 1197 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008431.2 |
RefSeq Size | 1757 bp |
RefSeq ORF | 1197 bp |
Locus ID | 16528 |
UniProt ID | O88454 |
Cytogenetics | 19 5.08 cM |
Gene Summary | Voltage-insensitive potassium channel (PubMed:9628867). Channel opening is triggered by mechanical forces that deform the membrane. Channel opening is triggered by raising the intracellular pH to basic levels (By similarity). The channel is inactive at 24 degrees Celsius (in vitro); raising the temperature to 37 degrees Celsius increases the frequency of channel opening, with a further increase in channel activity when the temperature is raised to 42 degrees Celsius (By similarity). Plays a role in the sensory perception of pain caused by pressure (PubMed:19279663). Plays a role in the perception of pain caused by heat (PubMed:19279663).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208813 | Kcnk4 (untagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4), (10ug) |
USD 503.00 |
|
MG221486 | Kcnk4 (tGFP-tagged) - Mouse potassium channel subfamily K member 4 (Kcnk4), (10ug) |
USD 657.00 |
|
MR221486 | Kcnk4 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4) |
USD 457.00 |
|
MR221486L3 | Lenti ORF clone of Kcnk4 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4) |
USD 757.00 |
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