Mum1l1 (NM_175541) Mouse Tagged ORF Clone

CAT#: MR221107

Mum1l1 (Myc-DDK-tagged) - Mouse melanoma associated antigen (mutated) 1-like 1 (Mum1l1), transcript variant 5

Product Images

Circular map for MR221107

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mum1l1
• Synonyms: 9430086K21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221107 representing NM_175541
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGCAAATATGTCCTGTGTAATTGGAAAGACCAATTATGGCCAGCAAAAGTTTTAGATAGGTCTG
  AAAGTCCATCAGAGAGTAAGAGGAAAAATACATCGTCCCTAGAAGTTGAAATACTTTCACTGGATGAGAA
  AATTACCGTGGAAAGCACAGACACAAAAGTCCTAAGCAAATCTGCTGTTGAAGCCATTATGTCCTCTCTA
  GCAGTACAGTCAGAGGTCATCATTGCACCTAGAGAGGAGACAGCCTATGAAAGATCACTGAAAATGGCAC
  TGGAAATGGTGAAAGAAGGAACCAATCTCAGCCAGGAAAGCATGTCAGAAGAACAACCTACAGCTACAGC
  GTCTGAAAATGTGCCAGAACAGCCACCTGATTCACCTCCTCATAAAAAGTTCCGCAAACTTGAAAGCAAC
  ACCCAGGAGGACTCTGCTTCCATACTGCTGTGCTCAGAGAGTGATGATTCCATGACCGATGATAAGTTGC
  AGGTGCACACAACCGGTGAGAGCATGCCAAGTGAGATGGACACAAAGGCAACAGAAAACTTAGGCTGTTG
  CCAAACAGACCCTTCCTTGGCAGATGAGGATGATAAAAAGGAAGAAAAGAAAAAGATTGACATCTCGGCA
  ATCATGTCTGTGAATTTATCACTCAAAGAAGAAAGCGAATATATTAAAGAAGAGAAATTCGTCCCCTCAT
  CAGAAGATCTCACTGTCCCCAAAGAGGAGTCACAAGACATCCTCCCAGAAGCGCCTCTGGCTGTTTCCTC
  TGAATGTTCTATTGTCTCAGAGAATAACATGGAAGATCCTGGTGAGGGCCCATCAAATCAGAATCTAGTC
  TCCTATGCCAACCAAAATCAGTCTTCTGTGGAATCCGATGTAGGTGCCGAGACATCCACTGCAGGGTGCT
  CAGGGGACTTTCAGGTTTCCCTTCCTACCCGTGATACAGTCAGCAGTGATCTACTGCTCCAGAGACTGGA
  TCTAGAAGATCTTGAGGAAGAAGCCCGAGCTTCAGGCAAGCTTTTGTCTTTAAATCCTGCCAGTGCCGCT
  GCGTTAGAAAATGATAATGAAGATGATGATGAAGACCTTCCACGTTTCATCCTCCATTATGAGACACGTG
  CATTTGAAACTGGAATGATAGTGTGGTTTAAATATCAAAAATACCCATTTTGGCCAGCAGTGATCAAAAG
  CATTAGGCGGAAAGAGAGGAAAGCGAGTGTGCTTTTGGTTGAGGCAGACATGAGTCCTCAAAAGAAAGGC
  GTTAGAGTATCTTTGAGGAGGCTGAAGAAATACGACTGTAAAGAGAAACAGGCACTAGTGGAGAAAGCCA
  GGGAGGAGTACCGGGAGAGTATCGATTGGTGCGTGTCACTGATTTGTGACTACCGAGTTAGACTAGGTTG
  TGGCTCTTTTACCGGCTCGTTCTTTGAGTATTATGCTGCTGACATCAGTTATCCAGTCAGGAAAATCATC
  AAACAAGATACCTTCAGAAATATATTTCCAAAGCTATACAATGAAAACGTGGGGGAGCAGTTGCCTATGG
  CATCCCAAGCCAAGAGAGTATCTTTCCAGAAAATTCTCCCTGATCGGATGAAGCCTGCTCGGGACCGAGC
  TAACAAGAACCTGGTAGATTTTATTGTCAATGCAAAAGGAACAGAGGACCACCTCTTGGGCATTTTAAAG
  GGCACCAAAAAATCCAAGTGGCTGAAATCATTTCTCAATGCAAAGAGTTTCACACCCTGTATTGAAACCT
  ACTTTGAAGATGAAGATCAACTGGATGAGGTAGTGAAATACCTACAAGAAATTTATAAGCAAATTGACCA
  GAAGATGCTGACTCTGATAAAAGATGACAAAATTAAGTTTGTCTTGGAAGTTCTTCTACCAGAAGCAATT
  ATTTGCTCAATTTCTGCTGTTGATGGCTTAGATTATGAGGCAGCTGAGGCAAAGTATCTAAAGGGACCAT
  CCCTCGGCTGTAGGGAGAGAGAATTATATGATTCCAAAATCCTATTTGAAAAGAGAAGGAGGTCATTACC
  AAACGAAGGCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221107 representing NM_175541
  Red=Cloning site Green=Tags(s)
  
  MDGKYVLCNWKDQLWPAKVLDRSESPSESKRKNTSSLEVEILSLDEKITVESTDTKVLSKSAVEAIMSSL
  AVQSEVIIAPREETAYERSLKMALEMVKEGTNLSQESMSEEQPTATASENVPEQPPDSPPHKKFRKLESN
  TQEDSASILLCSESDDSMTDDKLQVHTTGESMPSEMDTKATENLGCCQTDPSLADEDDKKEEKKKIDISA
  IMSVNLSLKEESEYIKEEKFVPSSEDLTVPKEESQDILPEAPLAVSSECSIVSENNMEDPGEGPSNQNLV
  SYANQNQSSVESDVGAETSTAGCSGDFQVSLPTRDTVSSDLLLQRLDLEDLEEEARASGKLLSLNPASAA
  ALENDNEDDDEDLPRFILHYETRAFETGMIVWFKYQKYPFWPAVIKSIRRKERKASVLLVEADMSPQKKG
  VRVSLRRLKKYDCKEKQALVEKAREEYRESIDWCVSLICDYRVRLGCGSFTGSFFEYYAADISYPVRKII
  KQDTFRNIFPKLYNENVGEQLPMASQAKRVSFQKILPDRMKPARDRANKNLVDFIVNAKGTEDHLLGILK
  GTKKSKWLKSFLNAKSFTPCIETYFEDEDQLDEVVKYLQEIYKQIDQKMLTLIKDDKIKFVLEVLLPEAI
  ICSISAVDGLDYEAAEAKYLKGPSLGCRERELYDSKILFEKRRRSLPNEGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175541
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175541.3, NP_780750.2
• RefSeq Size: 4523 bp
• RefSeq ORF: 2046 bp
• Locus ID: 245631
• UniProt ID: Q4VA55
• Cytogenetics: X F1
• MW: 77 kDa