Npff (NM_018787) Mouse Tagged ORF Clone
CAT#: MR220982
- TrueORF®
Npff (Myc-DDK-tagged) - Mouse neuropeptide FF-amide peptide precursor (Npff), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_018787" in other vectors (4)
Interest in protein/lysate? Submit request here!
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Npff |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220982 representing NM_018787
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCCAAGTGGGCTGCTCTGCTGCTGCTGCTACTGCTGCTGCTGAATTGGGGCCACACTGAAGAGG CAGGGAGCTGGGGTGAAGACCAAGTCTTTGCAGGAGAAGATAAGGGACCCCACCCACCACAGTATGCCCA CATTCCAGACAGGATCCAGACTCCTGGGTCCCTCTTTCGTGTTCTGCTCCAGGCCATGGACACACCTAGA AGGAGCCCAGCCTTCCTGTTTCAGCCCCAGAGGTTTGGCAGAAGTGCATGGGGGTCCTGGAGCAAGGAAC AGCTAAATCCGCAGGCCAGACAGTTCTGGAGCCTGGCCGCTCCTCAGCGCTTTGGGAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220982 representing NM_018787
Red=Cloning site Green=Tags(s) MDSKWAALLLLLLLLLNWGHTEEAGSWGEDQVFAGEDKGPHPPQYAHIPDRIQTPGSLFRVLLQAMDTPR RSPAFLFQPQRFGRSAWGSWSKEQLNPQARQFWSLAAPQRFGKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_018787 |
ORF Size | 342 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018787.1, NP_061257.1 |
RefSeq Size | 451 bp |
RefSeq ORF | 345 bp |
Locus ID | 54615 |
UniProt ID | Q9WVA8 |
Cytogenetics | 15 F3 |
MW | 13.5 kDa |
Gene Summary | This gene encodes neuropeptides involved in analgesia, cardiovascular regulation, neuroendocrine function and opiate addiction. The encoded protein is a preproprotein that undergoes further processing to generate multiple amidated peptides. [provided by RefSeq, Jul 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209987 | Npff (untagged) - Mouse neuropeptide FF-amide peptide precursor (Npff), transcript variant 1, (10ug) |
USD 165.00 |
|
MG220982 | Npff (tGFP-tagged) - Mouse neuropeptide FF-amide peptide precursor (Npff) transcript variant 1, (10ug) |
USD 350.00 |
|
MR220982L3 | Lenti ORF clone of Npff (Myc-DDK-tagged) - Mouse neuropeptide FF-amide peptide precursor (Npff), transcript variant 1 |
USD 450.00 |
|
MR220982L4 | Lenti ORF clone of Npff (mGFP-tagged) - Mouse neuropeptide FF-amide peptide precursor (Npff), transcript variant 1 |
USD 450.00 |
{0} Product Review(s)
Be the first one to submit a review