Bicral (NM_001100452) Mouse Tagged ORF Clone

CAT#: MR220876

Gltscr1l (Myc-DDK-tagged) - Mouse cDNA sequence BC032203 (BC032203)

Product Images

Circular map for MR220876

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bicral
• Synonyms: Gltscr1l; mKIAA0240
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220876 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGATGACGATGACTCCTGTCTCCTCGATCTTATTGGAGACCCACAAGCATTGAACTATTTTCTGC
  ACGGACCTAGCAGTAAATCGGGCAGCGATGATGTGACGAACGCAGGGTATTCTGCAGCCAATTCTAATTC
  AATTTTCGCCAACTCCACGAACGCTGACCCTAAATCGGCCCTCAAAGGTGTGAGTGACCAGCTTGGGGAG
  GGGCCCAGTGATGGGCTGCCGCTTGCAAGCAGCCTTCAGTTTCTTGAAGATGAACTTGAGTCTTCACCTC
  TCCCCGATCTCAGCGAGGACCAACCCTTTGACATTCTTCAGAAATCCTTGCAGGAGGCTAATATCACTGA
  ACAGACATTGGCAGAAGAGGCGTACCTGGATGCCAGTATAGGCTCAAGCCAACAGTTTGCACAAGCCCAG
  CTTCATCCTTCTTCATCAGCATCCTTTACTCAGGCTTCTAATGTTTCTAATTACTCAGGTCAGACACTGC
  AGCCTATCGGGGTGACTCACGTGCCTGTTGGAGCATCGTTTGCAAGCAATACAGTGGGTGTGCAGCATGG
  CTTTATGCAACACGTGGGGATCAGTGTTCCCAGCCAGCATTTGCCTAACAGCAGCCAGATTAGTGGCTCC
  GGTCAGATACAGTTAATCGGGTCCTTCGGTAATCAGCCTTCCATGATGACTATAAATAACCTCGATGGCT
  CTCAAATCATACTGAAAGGCAGTGGGCAGCAAGCCCCATCTAATGTGAGTGGGGGGCTTCTGGTTCACAG
  ACAGACTCCTAACGGCAACTCTCTGTTTGGGAACTCCACTTCCAGTCCTGTAGCACAGCCTGTCACCGTT
  CCATTTAACAGCACAAATTTCCAGGCATCTTTACCCGTGCATAACATCATTATTCAAAGGGGTCTTGCAC
  CAAATTCAAATAAAGTCCCAATTAATATCCAGCCAAAGCCGGTCCAGATGGGTCAGCAGAGCGCGTACAA
  TGTGAACAACCTTGGGATCCAGCAGCACCATGCCCAGCAGGGGATCTCCTTCGCCCCCACAAGCTCGCCC
  CAGGGCTCCGTGGTTGGGCCGCACATGTCTGTGAACATTGTCAACCAACAGAACACGAGAAAGCCTGTCA
  CCTCGCAGGCAGTGAGCGGCACAGGGGGCAGCATCGTCATCCATTCCCCCATGGGCCAGCCTCACACTCC
  CCAAAGTCAGTTCCTTATACCCACAAGCCTTTCTGTCAGCTCCAACTCGGTGCACCATGTCCAGGCTATA
  AACGGGCAGCTGCTTCAGACTCAGCCCTCCCAGCTCATCTCTGGCCAAGTGGCCTCTGAGCATGTCATGC
  TGAACAGGAATTCCTCTAACATGCTCAGGACCAACCAACCATATTCCGGACAGATGCTTAATAACCAGAA
  TACCGCCGTCCAGCTGGTGTCTGGGCAGACTTTTGCCACCTCTGGAAGTCCAGTGATAGTCAACCACGCC
  TCTCCTCAGATCGTCGGGGGACAGATGCCCTTGCAGCAGGCCTCACCCACCGTGTTACACCTGTCACCTG
  GGCAGAGCAGTGTTTCCCAGGGAAGGCCAGGCTTCGCCACCATGCCCGCGGTGAGCGGCATGGCAGGACC
  CGCTCGGTTCCCCGCCGTCAGCTCAGCTAGCACTGCTCATCCTACTCTTGGGCCTACGGTGCAGTCGGGG
  GCACCGGGATCAAACTTTACGGGAGACCAGCTGACACAAGCCAACAGAACGCCAGCGCCCGTCAGTGTGT
  CCCACCGTCTTCCAGTCTCTGCTTCCAAATCCCCCAGCACCTTGAGCAACACCCCGGGGACACAGCAGCA
  GTTCTTCTGTCAGGCTCAGAAGAAGTGTTTGAACCAGACCTCCCCCATTCCCACATCCAAGACCACAGAC
  GGCTTGAGGCCATCACAGATCCCTGGGCTCTTGAGCACCGCACTGCCAGGACAGGATTCTGGAAGCAAAA
  TTATGCCAGCGACCTTGGGGGCCACACAGGCACAACCAGAAAGCTCAGTTGGATCATCCCCGAGCCAGAC
  AGCTGTGCAGGTGGATAGTCATCCAGGACAGAAAAGGCCTGCTGCCAAACAGCTGACTAAAGGAGCTTTC
  ATCCTCCAGCAGTTACAGAGGGACCAAGCCCATGCTGTGACACCCGACAAAAGCCAGTTCCGGTCACTAA
  ATGACACGGTGCAGAGACTGCTCTCCTACCACGTGTGCCAGGGCTCCATGCCCACGGAGGAAGACCTGAG
  GCAAGTGGACAATGAATTTGAAGAGGTCGCCACTCAGCTCCTCAAAAGGACCCAAGCTATGCTGAACAAA
  TACAGATTCCTGCTCCTAGAAGACGCCATGAGGATCAACCCCTCTGCAGAGATGGTGATGATTGACAGGA
  TGTTCAACCAGGAGGAAAGAGCTTCCCTGTCGAGGGACAAGCGTCTGGCGCTCGTAGATCCTGAGGGTTT
  TCAGGCCGATTTCTGTTGTTCCTTCAAACTTGACGAAGCTGTACCTGAGACCCCGCTTGACAGGAGTGAC
  CAGCATCGCAGCAAAACCAGCTCGCTCCATCAGGTGCCCAGGGCCCAAAGCAGAGACCGAGCCAAGCCAG
  GCATGGCAGAAGCAACGAATCATGACCAGTTTCATCTAGTGCCTAACCACATCGTGGTCTCTGCAGAGGG
  AAACATTTCTAAAAAGTCAGAAGGCCACAGTAGAACACTGAAATTTGACAGAGGGGTCTTAGGCCAATAC
  CGGGGTCCGCCTGAGGACAAGGGCGGCCGGAGGGACCCTGCCAAGGTCAGCAGGTGCTCTCCGGGCCCCG
  AGGGCCACCGCAAAAGCTTGCCCAGGCCAGATCACGGCTCTGAGAGCAAGCTCCCCGGCGTCCTGGCCAG
  CTCGCACATGGAGATGCCCTGTCTCGACTCCTTCCAGGACAAAGCGCTGAGGAATTCCCCAAAGAATGAG
  GTTTTACACACAGACATCATGAAAGGGTCGGGTGAGCCCCAGCCAGATCTCCAGCTCACCAAGAGCCTAG
  AGAAAACCTTTAAGAACATCCTGGAACTCAAGAACTCGGGGCGGCCGCCAAGCGACCCTACGGCCAGCGG
  TGCGGCGGACCTGGACTTCCCCAGCTTTTCTCCAATGGCTTCGCAGGAAAACTGCCTAGAAAAATTCATC
  CCGGACCACAGTGAAGGCGTTGTAGAAACGGACTCCATTTTAGAAGCAGCTGTAAATAGTATTCTAGAGT
  GT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220876 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDDDDDSCLLDLIGDPQALNYFLHGPSSKSGSDDVTNAGYSAANSNSIFANSTNADPKSALKGVSDQLGE
  GPSDGLPLASSLQFLEDELESSPLPDLSEDQPFDILQKSLQEANITEQTLAEEAYLDASIGSSQQFAQAQ
  LHPSSSASFTQASNVSNYSGQTLQPIGVTHVPVGASFASNTVGVQHGFMQHVGISVPSQHLPNSSQISGS
  GQIQLIGSFGNQPSMMTINNLDGSQIILKGSGQQAPSNVSGGLLVHRQTPNGNSLFGNSTSSPVAQPVTV
  PFNSTNFQASLPVHNIIIQRGLAPNSNKVPINIQPKPVQMGQQSAYNVNNLGIQQHHAQQGISFAPTSSP
  QGSVVGPHMSVNIVNQQNTRKPVTSQAVSGTGGSIVIHSPMGQPHTPQSQFLIPTSLSVSSNSVHHVQAI
  NGQLLQTQPSQLISGQVASEHVMLNRNSSNMLRTNQPYSGQMLNNQNTAVQLVSGQTFATSGSPVIVNHA
  SPQIVGGQMPLQQASPTVLHLSPGQSSVSQGRPGFATMPAVSGMAGPARFPAVSSASTAHPTLGPTVQSG
  APGSNFTGDQLTQANRTPAPVSVSHRLPVSASKSPSTLSNTPGTQQQFFCQAQKKCLNQTSPIPTSKTTD
  GLRPSQIPGLLSTALPGQDSGSKIMPATLGATQAQPESSVGSSPSQTAVQVDSHPGQKRPAAKQLTKGAF
  ILQQLQRDQAHAVTPDKSQFRSLNDTVQRLLSYHVCQGSMPTEEDLRQVDNEFEEVATQLLKRTQAMLNK
  YRFLLLEDAMRINPSAEMVMIDRMFNQEERASLSRDKRLALVDPEGFQADFCCSFKLDEAVPETPLDRSD
  QHRSKTSSLHQVPRAQSRDRAKPGMAEATNHDQFHLVPNHIVVSAEGNISKKSEGHSRTLKFDRGVLGQY
  RGPPEDKGGRRDPAKVSRCSPGPEGHRKSLPRPDHGSESKLPGVLASSHMEMPCLDSFQDKALRNSPKNE
  VLHTDIMKGSGEPQPDLQLTKSLEKTFKNILELKNSGRPPSDPTASGAADLDFPSFSPMASQENCLEKFI
  PDHSEGVVETDSILEAAVNSILEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100452
• ORF Size: 3225 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100452.1, NP_001093922.1
• RefSeq Size: 6579 bp
• RefSeq ORF: 3225 bp
• Locus ID: 210982
• UniProt ID: Q8CHH5
• Cytogenetics: 17 C
• MW: 114.4 kDa
• Gene Summary: Component of SWI/SNF chromatin remodeling subcomplex GBAF that carries out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner.[UniProtKB/Swiss-Prot Function]