Ube2o (NM_173755) Mouse Tagged ORF Clone

CAT#: MR220692

Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (Ube2o)

Product Images

Circular map for MR220692

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ube2o
• Synonyms: 9630022H21; B230113M03Rik; E2-230K; mKIAA1734
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220692 representing NM_173755
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATCCCGCAGCGCCCGCACCCGCCCAGGCCCAGGCCGCCGCCGCCCCGACCCCGGCCGCAGCCC
  CCGCCGCCGCCGCCCCGCCGCCCGCGCCCGCTACGGACTCGGCCTCAGGGCCGTCCTCCGACTCCGGCCC
  CGAAGCAGGCTCACAGCGCCTGCTGTTCTCCCACGACCTGGTGTCCGGCCGTTACCGCGGCTCGGTGCAC
  TTCGGGCTGGTACGCCTCATCCACGGCGAGGACTCGGACTCGGAGGGCGACGATGACGGCCGCGGCAGCT
  CGGGCTGCTCCGAGGCCGGGGGCGCGGGCCACGAGGAGGGCCGGGCCAGCCCTCTGCGGCGCGGCTACGT
  GCGCGTCCAGTGGTACCCGGAGGGCGTCAAGCAGCATGTGAAGGAGACCAAGCTGAAACTGGAGGACCGC
  TCTGTGGTGCCCCGGGATGTGGTTCGACACATGCGCTCAACTGACAGCCAGTGTGGCACGGTGATAGATG
  TCAACATTGACTGTGCCGTCAAGCTCATCGGCACCAACTGCATCATCTACCCTGTCAACAGCAAGGACCT
  CCAGCACATCTGGCCCTTCATGTATGGAGACTATATCGCCTATGACTGCTGGCTGGGGAAGGTCTATGAC
  TTGAAGAACCAGATCATCCTCAAGTTGTCTAATGGTGCCAGGTGCTCCATGAACACAGAAGATGGTGCCA
  AACTCTACGATGTCTGCCCTCACGTCAGCGACTCGGGTCTCTTCTTTGATGACTCTTATGGCTTCTACCC
  AGGCCAAGTCCTTATTGGCCCAGCCAAGATCTTCTCCAGTGTCCAGTGGCTCTCTGGGGTCAAGCCTGTA
  CTCAGCACCAAGAGCAAGTTCCGTGTGGTGGTGGAGGAGGTGCAGGTTGTAGAACTGAAAGTCACGTGGA
  TTACCAAGAGCTTCTGCCCCGGCGGCACGGACAGCGTTAGCCCCCCACCTTCCATCATCACTCAGGAAAA
  CCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCAGCTTGGGGAACGCTGTCTGTAT
  GTCTTCCCAGCCAAGGTAGAGCCGGCCAAGATTGCCTGGGAATGTCCAGAAAAAAACTGCGCCCAGGGGG
  AGGGCTCTATGGCCAAGAAGGTGAAGCGTCTGTTGAAGAAGCAAGTTGTGAGAATCATGTCCTGCACCCC
  AGATACCCAGTGTCCTAGGGACCATTCCATGGAAGACCCAGACAAGAAAGGGGAAGCCAGAGCCGGGAGT
  GAAATAGGGTCTGCTAGCCCGGAGGAGCAACCTGATGGATCAGCCAGCCCCGTGGAGATGCAGGATGAGG
  GGTCGGAGGAGCTTCAGGAGACATGCGAGCCACTGCCCCCCTTCCTCTTGAAGGAGGGTGGAGATGACGG
  GCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCGGCTGACGATACAGATGACACCAGCTCTGTGACC
  TCCTCCGCCAGCTCTACTACCTCCTCCCAGAGTGGCAGTGGCACTGGTCGTAAAAAGAGCATTCCCTTGT
  CCATCAAAAACTTGAAGCGAAAACACAAGAGGAAGAAGAATAAGGTCACCCGTGACTTCAAGCCTGGAGA
  CAGGGTGGCAGTGGAGGTTGTGACCACGATGACCTCAGCTGATGTGATGTGGCAAGATGGCTCTGTGGAA
  TGCAACATCCGCTCCAACGACCTTTTCCCTGTGCACCACCTGGACAACAATGAGTTCTGCCCTGGGGACT
  TCGTGGTGGACAAGCGAGTCCAGAGCTGCCCAGACCCTGCTGTCTATGGTGTGGTGCAGTCTGGGGATCA
  TGTTGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGCGACCAAGTGGGGATGACGTGGAGCTCATTGGA
  GAAGAGGAGGACGTGAGCGTCTATGACATTGCTGACCACCCTGACTTTCGCTTCCGCACAACTGACATTG
  TCATCCGCATTGGTAACACGGAGGATGGGGCTCTACCCAAGGAGGATGAGCCCTCAGTGGGCCAGGTGGC
  CCGAGTGGATGTCAGCAGCAAAGTGGAAGTGGTGTGGGCTGACAACTCGAAAACCATCATCCTGCCACAG
  CACTTATACAACATCGAGTCTGAGATTGAGGAGTCTGACTACGACTCCGTGGAAGGCAGCTCCAGTGGGG
  CCTCCTCGGATGAGTGGGAGGACGACAGCGACAGCTGGGAGACCGACAACGGGCTCGTGGACGATGAGCA
  CCCCAAGATAGAGGAGCTGGCCGCCATCTTGCCTGCCGAGCAGCCCACTGCCCCCGAGGAGGACAAGGGC
  GTGGTCATCAGTGAGGAGGCGGCCACGGCTGCCATCCAGGGCGCCGTGGCCATGGCTGCCCCCGTGGCAG
  GGCTGATGGAGAAAGCTGGGAAGGACGGGCCTCCCAAGAGTTTCCGGGAGCTGAAGGAGGCCATCAAGAT
  CCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTCACAGGCTCGCCCACCTCGCCCACCGTGGAG
  CCTGAGAAGCCAACCCGAGAGAAGAAGTTTTTGGACGATATCAAGAAGCTACAGGAGAACCTCAAGAAGA
  CCCTGGACAATGTGGCCATTGCTGAGGAGGAGAAGATGGAGGCAGTACCCGACACAGAGCGCAAGGAGGA
  GAAGCCTGAGGTGCAGTCCCCAGTGAAAGCCGAGTGGCCCAGTGAGACACCTGTGCTCTGTCAGCAGTGC
  GGTGGCAGGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTCTCTGTGCTGGAGTTTGCACCCT
  CAAACCACTCTTTCAAGAAGATTGAGTTCCAGCCCCCGGAAGCCAAGAAGTTCTTCAGCACAGTGCGGAA
  GGAGATGGCTCTGCTGGCCACCTCGCTGCCCGATGGCATCATGGTCAAGACTTTTGAGGACAGAATGGAC
  CTCTTCTCTGCACTGATCAAGGGCCCCACTCGGACTCCCTATGAAGACGGCCTCTACCTATTTGACATCC
  AGCTTCCCAACATCTACCCTGCGGTGCCCCCCCACTTTTGCTACCTCTCCCAGTGCAGTGGCCGCCTGAA
  CCCCAACCTGTATGACAATGGGAAAGTGTGTGTCAGCCTCCTAGGTACCTGGATTGGAAAGGGGACAGAG
  AGGTGGACAAGCAAGTCTAGCCTTCTCCAGGTGCTCATCTCCATCCAAGGTCTGATCCTGGTGAATGAAC
  CATACTACAATGAAGCTGGCTTTGACAGTGACCGGGGCCTGCAGGAAGGCTATGAGAACAGTCGCTGCTA
  CAACGAGATGGCATTAATCCGTGTGGTGCAGTCCATGACCCAGTTGGTGCGACGACCTCCTGAGGTCTTC
  GAACAGGAGATTCGGCAGCACTTCAGTGTGGGTGGCTGGCGGCTGGTGAACCGAATTGAGTCCTGGCTGG
  AAACCCATGCCATGCAGGAGAGGGCCCAGGTGATGCCCAATGGGGCCCTTAAAGACAGCAGCTCTCTGGA
  GCCCATGGCTGCAGCTGAACTCTCAGACTCCGGCCGTGAAGAGCCTGAAGATGTTGGGATGGCCCCTGGG
  GAGGCGTCCCAGGGCTCAGACTCAGAGGGTGGAGCCCAGGGCCCTGCCTCAGCCAGCAGAGACCACACAG
  AGCAGACTGAGACAGCCCCTGATGCATCCGCACCTCCCAGCGTTAGACCAAAGAGGAGGAGGAAGAGCTA
  CCGCAGCTTCCTGCCTGAGAAGAGCGGTTACCCTGACATCGGCTTCCCCCTCTTCCCACTCTCCAAGGGT
  TTCATCAAGAGCATCCGGGGGGTCCTGACACAGTTCCGTGCTGCCCTGCTGGAGGCGGGCATGCCCGAAA
  GCACAGAGGACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220692 representing NM_173755
  Red=Cloning site Green=Tags(s)
  
  MADPAAPAPAQAQAAAAPTPAAAPAAAAPPPAPATDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVH
  FGLVRLIHGEDSDSEGDDDGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDR
  SVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYD
  LKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPV
  LSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLY
  VFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGS
  EIGSASPEEQPDGSASPVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVT
  SSASSTTSSQSGSGTGRKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVE
  CNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIG
  EEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQ
  HLYNIESEIEESDYDSVEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKG
  VVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVE
  PEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQC
  GGRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMD
  LFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTE
  RWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVF
  EQEIRQHFSVGGWRLVNRIESWLETHAMQERAQVMPNGALKDSSSLEPMAAAELSDSGREEPEDVGMAPG
  EASQGSDSEGGAQGPASASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKG
  FIKSIRGVLTQFRAALLEAGMPESTEDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173755
• ORF Size: 3864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173755.3, NP_776116.2
• RefSeq Size: 5192 bp
• RefSeq ORF: 3867 bp
• Locus ID: 217342
• UniProt ID: Q6ZPJ3
• Cytogenetics: 11 E2
• MW: 141.3 kDa
• Gene Summary: E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.[UniProtKB/Swiss-Prot Function]