Babam2 (NM_181280) Mouse Tagged ORF Clone

CAT#: MR220501

Bre (Myc-DDK-tagged) - Mouse brain and reproductive organ-expressed protein (Bre), transcript variant 4

Product Images

Circular map for MR220501

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Babam2
• Synonyms: 6030405P19Rik; AI429776; B830038C02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220501 representing NM_181280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGCCTGTAATGAATGGTATGGTGTGCCAGATCTAGAGAAAGCATCGTACCTTTGGAGAAAGAAAG
  AAAACCATCTGCCACTAGAAAAAGGCCAAAACTGTACATCATTGACTCCTGGACCCAACTGTGACCGCTT
  CAAACTGCACATCCCGTACGCTGGGGAGACGTTAAAATGGGACATAATTTTCAATGCTCAGTACCCAGAG
  CTGCCTCCTGATTTCATCTTTGGAGAGGATGCTGAGTTTCTGCCAGACCCCTCTGCGCTGCACAACCTTG
  CCTCCTGGAACCCTTCAAACCCTGAGTGCCTGTTGCTCGTGGTGAAGGAGCTGGTGCAGCAGTACCACCA
  GTTCCAGTGCGGCCGCCTCCGTGAGAGCTCGCGCCTCATGTTTGAGTACCAGACGCTCCTGGAAGAGCCT
  CAGTATGGAGAGAACATGGAAATTTATGCTGGAAAGAAAAACAACTGGACTGGTGAATTTTCAGCTCGTT
  TTCTATTGAAGTTACCAGTAGATTTCAGCAACATTCCCACATACCTTCTCAAGGATGTAAATGAAGACCC
  TGGAGAAGATGTGGCCCTTCTTTCTGTCAGTTTTGAGGATACTGAAGCTACCCAGGTGTACCCCAAGTTG
  TACTTGTCACCCCGAATTGAGCATGCACTCGGAGGCTCCTCTGCTCTTCACATCCCTGCTTTCCCCGGAG
  GAGGATGTCTCATTGATTATGTGCCTCAAGTGTGCCACCTGCTCACCAACAAGGTACAGTATGTGATTCA
  AGGCTATCACAAAAGAAGAGAGTACATCGCAGCTTTCCTCAGTCACTTTGGCACAGGTGTCGTGGAATAT
  GATGCAGAAGGCTTCACAAAACTTACTCTGCTGCTGATGTGGAAAGACTTTTGTTTTCTTGTCCACATTG
  ACCTGCCCCTGTTTTTCCCTCGAGATCAGCCTACGCTCACATTTCAGTCAGTCTATCACTTTACCAACAG
  TGGACAGCTTTACTCCCAAGCACAAAAAAACTACCCATACAGCCCCAGATGGGATGGAAATGAAATGGCC
  AAGAGAGCAAAGGCTTATTTCAAAACCTTTGTCCCTCAGTTCCAGGAGGCCGCATTTGCCAATGGAAAGC
  TC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220501 representing NM_181280
  Red=Cloning site Green=Tags(s)
  
  MCACNEWYGVPDLEKASYLWRKKENHLPLEKGQNCTSLTPGPNCDRFKLHIPYAGETLKWDIIFNAQYPE
  LPPDFIFGEDAEFLPDPSALHNLASWNPSNPECLLLVVKELVQQYHQFQCGRLRESSRLMFEYQTLLEEP
  QYGENMEIYAGKKNNWTGEFSARFLLKLPVDFSNIPTYLLKDVNEDPGEDVALLSVSFEDTEATQVYPKL
  YLSPRIEHALGGSSALHIPAFPGGGCLIDYVPQVCHLLTNKVQYVIQGYHKRREYIAAFLSHFGTGVVEY
  DAEGFTKLTLLLMWKDFCFLVHIDLPLFFPRDQPTLTFQSVYHFTNSGQLYSQAQKNYPYSPRWDGNEMA
  KRAKAYFKTFVPQFQEAAFANGKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181280
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181280.1, NP_851797.1
• RefSeq Size: 1308 bp
• RefSeq ORF: 1125 bp
• Locus ID: 107976
• UniProt ID: Q8K3W0
• Cytogenetics: 5 B1
• MW: 43.4 kDa
• Gene Summary: Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin (By similarity). May regulate TNF-alpha signaling through its interactions with TNFRSF1A.[UniProtKB/Swiss-Prot Function]