Fmo3 (NM_008030) Mouse Tagged ORF Clone
CAT#: MR220435
- TrueORF®
Fmo3 (Myc-DDK-tagged) - Mouse flavin containing monooxygenase 3 (Fmo3)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_008030" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Fmo3 |
Synonyms | AW111792 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220435 representing NM_008030
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGAAAGTGGCCATCATTGGAGCTGGTGTCAGTGGCCTGGCTGCCATCAGGAGCTGTCTGGAGG AGGGGCTGGAGCCCACATGCTTTGAGAGGAGTGATGATGTTGGGGGCCTGTGGAAATTCTCAGACCATAT AGAAGAGGGCAGGGCCAGCATTTACCAATCGGTCTTCACCAACTCTTCCAAAGAGATGATGTGTTTTCCA GACTTCCCCTATCCCGATGACTTTCCCAACTTCATGCATCACAGCAAGCTCCAAGAATACATCACTTCAT TTGCCAAGGAAAAGAACCTCCTGAAATACATACAGTTTGAGACACCTGTAACCAGTATAAATAAATGTCC TAATTTCTCAACTACTGGCAAATGGGAAGTCACCACTGAAAAGCACGGTAAAAAAGAAACAGCTGTCTTT GATGCTACAATGATTTGTTCTGGGCATCACATATTTCCCCATGTACCAAAAGACTCCTTTCCAGGACTGA ACCGTTTTAAAGGCAAATGCTTCCACAGCAGGGACTATAAGGAACCAGGAATATGGAAGGGAAAACGAGT CCTGGTGATTGGCCTGGGGAACTCAGGCTGTGACATTGCTGCAGAACTCAGCCATGTAGCTCAGAAGGTC ACCATCAGCTCTAGAAGTGGTTCTTGGGTGATGAGTCGAGTCTGGGACGATGGCTACCCTTGGGACATGG TGGTGCTCACACGGTTTCAAACTTTCCTCAAAAACAACTTACCCACCGCCATCTCTGACTGGTGGTACAC AAGGCAGATGAATGCCAGATTCAAGCACGAAAACTATGGTTTGGTGCCTTTAAACAGAACACTCAGGAAA GAGCCCGTGTTCAATGATGAGCTCCCAGCCCGCATCCTGTGTGGCATGGTGACCATCAAGCCTAATGTAA AGGAGTTCACAGAGACGTCCGCTGTGTTTGAGGATGGGACCATGTTTGAGGCCATTGACTGTGTCATCTT TGCCACAGGCTATGGTTATGCCTACCCCTTCCTGGATGACTCTATTATCAAAAGCAGAAATAATGAGGTC ACTTTGTACAAAGGTGTCTTCCCTCCTCAACTAGAGAAACCAACCATGGCAGTGATTGGCCTGGTCCAGT CCCTGGGTGCCACCATCCCCATAACTGACCTGCAGGCACGCTGGGCAGCACAAGTAATAAAAGGAACTTG CACTTTGCCTTCTGTAAACGACATGATGGATGACATTGATGAGAAAATGGGGGAAAAGTTCAAATGGTAT GGCAATAGCACCACCATCCAGACAGATTACATTGTTTATATGGATGAACTGGCCTCCTTCATTGGTGCAA AGCCCAATCTCCTATGGCTGTTTCTCAAGGATCCCAGGTTGGCTGTAGAAGTGTTCTTTGGCCCTTGCAG CCCCTACCAGTTCCGGCTGGTAGGCCCAGGAAAGTGGTCAGGAGCCCGGAACGCCATCCTAACACAGTGG GACCGATCACTGAAGCCTATGAAGACGCGTGTCGTCAGTAAAGTTCAGAAGTCTTGCTCTCACTTCTATT CCCGTTTGCTCAGGCTCCTGGCTGTTCCCGTTCTGCTCATTGCTTTGTTCCTTGTGTTGATC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR220435 representing NM_008030
Red=Cloning site Green=Tags(s) MKKKVAIIGAGVSGLAAIRSCLEEGLEPTCFERSDDVGGLWKFSDHIEEGRASIYQSVFTNSSKEMMCFP DFPYPDDFPNFMHHSKLQEYITSFAKEKNLLKYIQFETPVTSINKCPNFSTTGKWEVTTEKHGKKETAVF DATMICSGHHIFPHVPKDSFPGLNRFKGKCFHSRDYKEPGIWKGKRVLVIGLGNSGCDIAAELSHVAQKV TISSRSGSWVMSRVWDDGYPWDMVVLTRFQTFLKNNLPTAISDWWYTRQMNARFKHENYGLVPLNRTLRK EPVFNDELPARILCGMVTIKPNVKEFTETSAVFEDGTMFEAIDCVIFATGYGYAYPFLDDSIIKSRNNEV TLYKGVFPPQLEKPTMAVIGLVQSLGATIPITDLQARWAAQVIKGTCTLPSVNDMMDDIDEKMGEKFKWY GNSTTIQTDYIVYMDELASFIGAKPNLLWLFLKDPRLAVEVFFGPCSPYQFRLVGPGKWSGARNAILTQW DRSLKPMKTRVVSKVQKSCSHFYSRLLRLLAVPVLLIALFLVLI SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008030 |
ORF Size | 1602 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008030.2, NP_032056.1 |
RefSeq Size | 2020 bp |
RefSeq ORF | 1605 bp |
Locus ID | 14262 |
UniProt ID | P97501 |
Cytogenetics | 1 70.34 cM |
MW | 61 kDa |
Gene Summary | Essential hepatic enzyme that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including drugs as well as dietary compounds. Plays an important role in the metabolism of trimethylamine (TMA), via the production of trimethylamine N-oxide (TMAO) metabolite. TMA is generated by the action of gut microbiota using dietary precursors such as choline, choline containing compounds, betaine or L-carnitine. By regulating TMAO concentration, FMO3 directly impacts both platelet responsiveness and rate of thrombus formation.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217562 | Fmo3 (untagged) - Mouse flavin containing monooxygenase 3 (Fmo3), (10ug) |
USD 548.00 |
|
MG220435 | Fmo3 (tGFP-tagged) - Mouse flavin containing monooxygenase 3 (Fmo3), (10ug) |
USD 697.00 |
|
MR220435L3 | Lenti ORF clone of Fmo3 (Myc-DDK-tagged) - Mouse flavin containing monooxygenase 3 (Fmo3) |
USD 797.00 |
|
MR220435L4 | Lenti ORF clone of Fmo3 (mGFP-tagged) - Mouse flavin containing monooxygenase 3 (Fmo3) |
USD 797.00 |
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