Galnt15 (NM_030166) Mouse Tagged ORF Clone
CAT#: MR220195
- TrueORF®
Galnt15 (Myc-DDK-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2 (Galntl2)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_030166" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Galnt15 |
Synonyms | 4631401E18Rik; Galntl2; mpp-GalNAc-T15 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220195 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCCGAGGAAGCGACCCAGGAGTGGACGTTCCAGGCTTCAGTTCCTCCTACTCTTCCTGACTCTGG GATGTGTCCTGATGATGGTGATCCTGCTGCATCCTCCCCCGCCCACCCTGCACCAGGCTGTCACAGCTCA GGCCAGCAAACACAGCCCTGACACGGGGTACCGCCTGGACTTTGGAGACTCTCAGGAGTGGGTCCTGGAG GCTGAGACTGAAGGTGATGAGTACAGTCTCTTGGATGGCCTGCCATCCTTCATCTCCCTGCAGGAGGATC AACTGCTGGTGGCCGTGGCCTCACCCAGAGCTAGGAGGAGTCAGAGCCAGGGCAGGAGACAGGGCAGCTA CCAGTTCATCAAACACAGGAGCAGGAGATGGGATGAGGAAGCCCTGGAAAAGGACTGGAGGACCGAAGAG GATGGGGAGGAATCAGAAGAGGTGCTGACCCCACTTGGCCCGGACTCAGATGGCCTCAACAAGCCACTCA GTGCCCGCCTGCCCCTGAGGAGAGTCCTGCCCGAGGTGCGACACCCACTGTGTCTGCAGCAGCACCCTAC AAGTGGCCTGCCTACAGCCAGCGTCATCCTCTGCTTCCATGATGAGGCCTGGCCCACGCTCTTGAGAACT GTCCACAGCATCCTGGACACAGCACCCAGGGCCCTGCTGCAGGAGATCATCCTTGTAGATGACCTCAGCC AGCAAGAACTGCTGAAGTCTGCTCTCAGTGAATATGTGGCCAGGCTGGAGGCTGTGAAGTTGCTCAGGAG CAATAGGAGGCTCGGCACCATTGGAGCCCGGATGCTGGGGGCCACCAGGGCCACAGGGGACGTGCTGGTC TTCATGGATGCCCACTGTGAGTGCCACCCTGGTTGGCTGGAGCCCCTCCTCAGCAGAATAGCTGATGACA GGAGCCGAGTGGTGTCTCCAGTCATAGACGTGATTGACTGGAAGACGCTTCAGTACTCCGCATCCAAGCT GCATCGAGGGACGCTGGATTGGAAGCTGGATTTCCGATGGAAGCCCCTGGGGGAGCAGGAGCAGAAGGCC CTCCCATCCCCCATCAGCCCTGTCAGGAGCCCTGTGGTACCCCGAGAGGTGGTGGCCGTGGACAGACATT ACTTCCAAAACACCGGAGCATACGATCCCCTTCTGTCACTGGGGGATAGTGAAAATCTCGAGATGTCTTT CAAGGCCTGGCTCTGCGGTGGCTCTGTTGAAATCCTTCCCTGCTCTCGAGTGGGACACATCTACCGAAGC CAAGATGCCAGCTCCAGGCCTGACCCCGAGGTCGCCTTGAAGAACAAGATCATCATTGCTGAGACCTGGC TATCATCCTTCAAGGAAACCTTCTACAGGCACATCCCAGAGGCCTTCACCCTGAGCAAGGTTGCAAAGCC AGACTGTACAGAGCGCCTGAAGCTGCAAAGGAGGCTGGGATGCCGGACGTTCCACTGGTTTCTGGCCAAT GTCTACCCTGAGCTGTACCCATCTGATCACAGGCCCAGGTTCTCTGGAAAGCTCCACAACACTGGATTTG GCCTCTGTGCGGACTGCCAAGCAGACGGTGACATCCTGGGCTGCCCCATGACACTGGCTCCTTGCAGTAA CAACCGGCAGCAACAGAACCTGGAGCACACCGGCAGGAAGGAGATTCTCTTCGGCGGCCCACAGCGCCTG TGCTTCGATGTCAGAGGAGGGCGGGTGATTCTGCAGAACTGCACGGAGGAAGGCCCCGCTATCCATCAGC AGCACTGGGACTTCCAGGAGGATGGAATGATCATCCATGTTCTTTCTGGAAAATGCATGGAAGCTGGGGT CCAGCCAAGCAATAAGGACTTGTACTTGCGTCAATGTGATGGTAAAACCAGCCAGCTGTGGAGGTTCGAC CAGATCCACCCTGTGGATGAACGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220195 protein sequence
Red=Cloning site Green=Tags(s) MLPRKRPRSGRSRLQFLLLFLTLGCVLMMVILLHPPPPTLHQAVTAQASKHSPDTGYRLDFGDSQEWVLE AETEGDEYSLLDGLPSFISLQEDQLLVAVASPRARRSQSQGRRQGSYQFIKHRSRRWDEEALEKDWRTEE DGEESEEVLTPLGPDSDGLNKPLSARLPLRRVLPEVRHPLCLQQHPTSGLPTASVILCFHDEAWPTLLRT VHSILDTAPRALLQEIILVDDLSQQELLKSALSEYVARLEAVKLLRSNRRLGTIGARMLGATRATGDVLV FMDAHCECHPGWLEPLLSRIADDRSRVVSPVIDVIDWKTLQYSASKLHRGTLDWKLDFRWKPLGEQEQKA LPSPISPVRSPVVPREVVAVDRHYFQNTGAYDPLLSLGDSENLEMSFKAWLCGGSVEILPCSRVGHIYRS QDASSRPDPEVALKNKIIIAETWLSSFKETFYRHIPEAFTLSKVAKPDCTERLKLQRRLGCRTFHWFLAN VYPELYPSDHRPRFSGKLHNTGFGLCADCQADGDILGCPMTLAPCSNNRQQQNLEHTGRKEILFGGPQRL CFDVRGGRVILQNCTEEGPAIHQQHWDFQEDGMIIHVLSGKCMEAGVQPSNKDLYLRQCDGKTSQLWRFD QIHPVDER TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_030166 |
ORF Size | 1917 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030166.3, NP_084442.1 |
RefSeq Size | 2341 bp |
RefSeq ORF | 1917 bp |
Locus ID | 78754 |
UniProt ID | Q9D2N8 |
Cytogenetics | 14 B |
MW | 72.3 kDa |
Gene Summary | Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Although it displays a much weaker activity toward all substrates tested compared to GALNT2, it is able to transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting that it can fill vicinal Thr/Ser residues in cooperation with other GALNT proteins. Prefers Muc1a as substrate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220021 | Galnt15 (untagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2 (Galntl2), (10ug) |
USD 654.00 |
|
MG220195 | Galnt15 (tGFP-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2 (Galntl2), (10ug) |
USD 795.00 |
|
MR220195L3 | Lenti ORF clone of Galnt15 (Myc-DDK-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2 (Galntl2) |
USD 895.00 |
|
MR220195L4 | Lenti ORF clone of Galnt15 (mGFP-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2 (Galntl2) |
USD 895.00 |
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