Ankef1 (NM_175667) Mouse Tagged ORF Clone

CAT#: MR219992

Ankef1 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 5 (Ankrd5)

Product Images

Circular map for MR219992

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankef1
• Synonyms: Ankrd5; AV276460
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219992 representing NM_175667
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGGCAGACAAGAGACTTGAGAACTTACAGATCTACAGAGTTCTTCAGTGTGTTCGCAACAAAG
  ACAAGAAGCAGATAGAAAAGCTGACCAGGCTTGGGTACCCTGAGCTAATCAACTTCACCGAACCCATAGA
  TGGGCTGAGTGCTCTCCACTTAGCCTCCATTTCCAACGACACTGACATGGTCAGCTTCCTCCTCAAACTT
  GGTGCGCATCCTGATGTGCAAGACCACATGGGCTGTACCCCGACCATGAGAGCTGCCGAGCTGGGCCATG
  AGTTGTCAATGGAAATTTTAGCCAAGGCCAAAGCTGATATGACAATAGTTGATAATGAAGGAAAAGGTGT
  TCTGTTTTACTGCATCCTGCCCACTAAGCGGCACTATCGATGCTCACTGATTGCCTTGGAGCATGGTGCA
  GATGTCAACAATATCACCTATGAAGGGAAGCCAGTGTTCCTCAGAGCTTGCGAGGAAGCACATGATGTGA
  AGGATATGTGCCTGACATTTTTGGAGAAAGGAGCCAATCCTAATGCTATCAACACATCCACAGGACGCAC
  GGCTTTGATGGAATCGTCAAGAGAAGGGGTGCTGGAAATAGTTCGTGGCATATTGGAAAGAGGAGGTGAA
  GTGAACGCATATGACAATGATAGACACCACGCTGCTCATTTTGCTGCCAAAGGAGGCTTTTTTGATATTC
  TGAAGCTCCTCTTTGCCTACAATGGAGACATGGGGTTAATTGGAATGGATGGGAACACACCACTTCACTT
  TGCTGCCATGGGGGGTTTTGCAGATTGCTGTAAATATATAGCTCAGCGAGGATGTGACCTGAAATGGAAA
  AATTTGGAACATAAAACACCCCGAGTTGTGGCGAAAGACGGAGGCTTTAAAGCTGCTAGCAAGGAAATAC
  GTCGAGCAGAGCGGACTGCCGCTAAACTCGCTAAGACAGGAGCAAAAAATCCTAACCCTCTCTGGGCCCT
  CAGGCTGCATGACTGGTCCATAGAGCACGAGACTTCCCTTCGGAACGCCTTTAAGTTCGTGGACAGGGGC
  GATGGCGTAGTCAGCAAAGATGACTTCGTGGTGGCCCTGGAGGAGAGGCAAGAGTATGCCACCTCAGAGC
  AGTTGCTTTCCGTTGCTCAAATGCACGAAAAAAGTCGAGGAGGCGGGGTCAACATTAATGAGTTCTTTAA
  GGGAACCAAATATTTAAGCAAGTCGTACGTATTGGGATCTTTCGGGCCTAAGAAAAAGAAAAGGGGGTTG
  GGCAAAAAGCCAAGGAAAGGCAAGTTTGTTTTGCCTCTCCCCATCTGCACCATCCCGGAGAACGCCTTCC
  CGCGGCGGCCAGATGGGGGCCCGCCCTACTACATGATTGAGACCTACCAGAATGTCTCCGACAGCCACAG
  GTTCAACCGGGACCACCCGCCCGAGCACCCAATCCAGGATGATTCCGAGTGGTACATCGATGATCCGAGT
  AGGGTCTTCGCAAATATTAGTTTTATCACCAAAGCAGGAGATCTGGCCTCTCTGAAAAAGGCCATCGAAA
  CAGGAATACCCGTGGATATGAAGGACAATACTTACAAGACTCCTCTCATGATAGCGTGTGCCAGTGGCAA
  CATTGATGTGGTCAAGTTTCTTATCGAAAAGGGGGCAAATGTTAATGCAACAGATAATTTTCTATGGACT
  CCACTTCATTTTGCATGCCATGCTGGCCAGCAAGACATTGTTGAGCTTCTTGTTAAAGCTGGAGCTTCAA
  TAGATGCAACATCAATCAACAACTCCACTCCGTTAAGCAGAGCCATTGAAAGCTGTAGGCTGGACACTGT
  AAAATACCTACTTGATATGGGTGCCAAATTCCAGATTGAAAACAGAAAAGGACATGCTGCCATGGATATT
  GCAAAGGCATATGCTGATTATAGAATAATTGATATGATAAAAGAAAAGCTAGATAACCTGCCTAAACAAG
  CAGATAATCAGAAAATGAAAGGCAAGCTTCCTAAACTGAAGACAGAAGGCACGGATGTTAAGAAAGAAGA
  GGAAACACTGTCATCCATTTACACTGTGCCAGCAATAACAGAGGAGAAGAAAGTCCACAGGGATAGTGTG
  GTTTACCTCAATTCCCTGATTACCAGTGGCTTCACCAAGAAAGTTGATATCACATTTATCCCCAAAAGGA
  TTTGGAGTCCTGAAGCCACAACAGCAGAGCTGATCAGGAAGAGGGAGCTCCGGAGGGAGAGGTTCACATA
  CGAGGTGGACTTTGAAGACTTCATGATGCCTTTCCAAAAGAACATCACGGAGAAAGCTCAGGCACTGGAG
  GCAACACTCAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219992 representing NM_175667
  Red=Cloning site Green=Tags(s)
  
  MALADKRLENLQIYRVLQCVRNKDKKQIEKLTRLGYPELINFTEPIDGLSALHLASISNDTDMVSFLLKL
  GAHPDVQDHMGCTPTMRAAELGHELSMEILAKAKADMTIVDNEGKGVLFYCILPTKRHYRCSLIALEHGA
  DVNNITYEGKPVFLRACEEAHDVKDMCLTFLEKGANPNAINTSTGRTALMESSREGVLEIVRGILERGGE
  VNAYDNDRHHAAHFAAKGGFFDILKLLFAYNGDMGLIGMDGNTPLHFAAMGGFADCCKYIAQRGCDLKWK
  NLEHKTPRVVAKDGGFKAASKEIRRAERTAAKLAKTGAKNPNPLWALRLHDWSIEHETSLRNAFKFVDRG
  DGVVSKDDFVVALEERQEYATSEQLLSVAQMHEKSRGGGVNINEFFKGTKYLSKSYVLGSFGPKKKKRGL
  GKKPRKGKFVLPLPICTIPENAFPRRPDGGPPYYMIETYQNVSDSHRFNRDHPPEHPIQDDSEWYIDDPS
  RVFANISFITKAGDLASLKKAIETGIPVDMKDNTYKTPLMIACASGNIDVVKFLIEKGANVNATDNFLWT
  PLHFACHAGQQDIVELLVKAGASIDATSINNSTPLSRAIESCRLDTVKYLLDMGAKFQIENRKGHAAMDI
  AKAYADYRIIDMIKEKLDNLPKQADNQKMKGKLPKLKTEGTDVKKEEETLSSIYTVPAITEEKKVHRDSV
  VYLNSLITSGFTKKVDITFIPKRIWSPEATTAELIRKRELRRERFTYEVDFEDFMMPFQKNITEKAQALE
  ATLKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175667
• ORF Size: 2325 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175667.4, NP_783598.1
• RefSeq Size: 2723 bp
• RefSeq ORF: 2328 bp
• Locus ID: 319196
• UniProt ID: Q9D2J7
• Cytogenetics: 2 F3
• MW: 86.9 kDa