Xrcc3 (NM_028875) Mouse Tagged ORF Clone
CAT#: MR219979
- TrueORF®
Xrcc3 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_028875" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Xrcc3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219979 representing NM_028875
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTGGATCAGCTGGACCTAAACCCCCGAATTACTGCTGCGGTTAAGAGGGGCAGACTGAAGTCAG TGAAGGAGATTCTGTGCTACTCCGGACCAGACTTGCAGCGGCTCACCGGCCTGCCCAGCCACGATGTGCA GTGCCTGCTGAGAGCTGCCTCTCTACACCTTCGGGGAAGCCGCGTCCTCTCAGCACTGCATCTGTTCCAG CAGAAGGAGAGCTTCCCTGAGCAGCATCAACGCCTGAGCCTAGGCTGCCCTGTCCTGGATCAGTTCCTGG GTGGAGGCCTGCCCCTGGAGGGCATCACTGGCCTGGCTGGCTGCAGCTCAGCAGGAAAGACCCAGCTGGC GCTACAGCTCTGCCTGGCTGTGCAGTTCCCAAGACAATATGGAGGCCTAGAGGCCGGGGCTGTCTACATC TGCACAGAAGATGCCTTCCCCAGCAAGCGGCTGTGGCAGCTCATCGCGCAGCAGCGGAGGCTGCGGACAG ACGCGCCTGAGGAGCTGATCGAGAAGATCAGGTTCAGCAACCACATCTTCATTGAACACGCGGCCGACGT GGACACCTTGTTGGAGTGTGTGAGTAAGAAAGTCCCCATCCTGCTGTCGAGGGGCATGGCCCGCCTGGTG GTGGTTGACTCCATTGCAGCCCCATTTCGTTGTGAGTTCCACCTTCAGGCCTCAGCCATCAGGGCGAAGC TCCTGCTCTCGCTGGGGGCCACACTGCGAAGACTGAGCAGCACCTTCAGGAGCCCAGTGCTGTGCATCAA CCAGGTGACAGACATGGTGGAGGACCAGCAGTCTGTGTCCAGGTCACTAGGGGCTTCAGAAGAGCGCCTC TCTCCAGCCCTTGGCATCACCTGGGCTAACCAGCTCCTGATGCGACTGATGGTTGACCGCACCCATGAGG ACGATGTTACCACAGGCTTGCCCAGAAGCCCAGTTCGGACTCTCCGAGTGCTCTTCGCCCCCCACCTACC CCTCTCCTCCTGCTGCTACACAGTCAGTGGGGAAGGCATTAGGGGGATGCCAGGAACACAGTCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219979 representing NM_028875
Red=Cloning site Green=Tags(s) MDLDQLDLNPRITAAVKRGRLKSVKEILCYSGPDLQRLTGLPSHDVQCLLRAASLHLRGSRVLSALHLFQ QKESFPEQHQRLSLGCPVLDQFLGGGLPLEGITGLAGCSSAGKTQLALQLCLAVQFPRQYGGLEAGAVYI CTEDAFPSKRLWQLIAQQRRLRTDAPEELIEKIRFSNHIFIEHAADVDTLLECVSKKVPILLSRGMARLV VVDSIAAPFRCEFHLQASAIRAKLLLSLGATLRRLSSTFRSPVLCINQVTDMVEDQQSVSRSLGASEERL SPALGITWANQLLMRLMVDRTHEDDVTTGLPRSPVRTLRVLFAPHLPLSSCCYTVSGEGIRGMPGTQSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028875 |
ORF Size | 1047 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028875.3, NP_083151.1 |
RefSeq Size | 2422 bp |
RefSeq ORF | 1050 bp |
Locus ID | 74335 |
UniProt ID | Q9CXE6 |
Cytogenetics | 12 F1 |
MW | 38.9 kDa |
Gene Summary | This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. Allelic variants in the human gene are associated with susceptibility to breast cancer and cutaneous malignant melanoma. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211524 | Xrcc3 (untagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3), (10ug) |
USD 503.00 |
|
MG219979 | Xrcc3 (tGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3), (10ug) |
USD 657.00 |
|
MR219979L3 | Lenti ORF clone of Xrcc3 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3) |
USD 757.00 |
|
MR219979L4 | Lenti ORF clone of Xrcc3 (mGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3) |
USD 757.00 |
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