Anks1b (NM_001177397) Mouse Tagged ORF Clone
CAT#: MR219871
- TrueORF®
Anks1b (Myc-DDK-tagged) - Mouse ankyrin repeat and sterile alpha motif domain containing 1B (Anks1b), transcript variant 4
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001177397" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Anks1b |
Synonyms | AIDA-1b; C030032C09Rik; E530015N03Rik; ENSMUSG00000078431; Gm1555; Gm10937 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219871 representing NM_001177397
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAATGGATTTGACAGTGTACAGTTTATGGGAAGCAATGTTATGGAAGATCAGGATTTGTTGGAAA TTGGAATCCTTAACTCTGGCCACAGACAGAGAATTCTACAAGCGATCCAGCTCCTTCCAAAGATGAGGCC CATCGGCCATGATGGCTACCATCCCACCTCTGTAGCCGAGTGGCTGGATTCCATTGAGCTGGGTGATTAC ACCAAAGCCTTTCTCATCAATGGCTACACGTCCATGGACCTGTTGAAAAAGATCTGGGAGCTTGAGCTTA TTAATGTTTTAAAAATCAGTTTGATTGGCCACAGGAAACGCATTTTGGCATCTTTGGGAGATAGGCTGCA CGACGACCCTCCACAGAAGCCCCCTCGGTCCATCACCCTCAGGGAACCCAGTGGTAATCACACTCCTCCT CAGTTGTCTCCATCACTTAGCCAAAGCACTTACACCACTGGTGGCTCCCTAGACGTTCCTCACATTATCA TGCAGGGCGATGCAAGGAGGAGAAGAAATGAAAACTACTTTGATGATATTCCCCGATCAAAGCTGGAGAG GCAGATGGCTCAGACAGGGGACTGGGGAGAACCTTCCATCACCTTGCGACCTCCTAATGAAGCCACAGCC TCAACTCCCGTCCAGTACTGGCAGCATCATCCGGAAAAACTCATCTTCCAGTCATGTGACTACAAAGCCT TTTATTTAGGTTCTATGCTGATAAAAGAGCTGAGGGGGACAGAATCAACACAGGATGCTTGTGCAAAGAT GCGGGCTAACTGTCAGAAGTCTACAGAGCAAATGAAGAAAGTCCCCACTATCATCCTTTCCGTCTCATAT AAAGGAGTAAAATTTATCGATGCGGCAAATAAGAATATAATCGCCGAACATGAAATTCGTAATATCTCCT GTGCTGCCCAGGACCCAGAAGACCTCTCAACATTTGCCTATATCACAAAAGATTTGAAGTCCAACCACCA CTACTGTCACGTGTTTACTGCCTTTGATGTGAATTTAGCCTATGAAATCATCCTGACGCTGGGACAGGCA TTTGAGGTCGCTTACCAACTAGCCCTACAAGCAAGAAAAGGGGGCCATTCCTCCACACTCCCAGAAAGCT TTGAGAACAAGCCCTCGAAACCCATCCCAAAGCCCCGAGTTAGCATTCGGAAGTCAGTGGCTCCGCAGAC ATCCTGTCCCAACAGGCTGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219871 representing NM_001177397
Red=Cloning site Green=Tags(s) MANGFDSVQFMGSNVMEDQDLLEIGILNSGHRQRILQAIQLLPKMRPIGHDGYHPTSVAEWLDSIELGDY TKAFLINGYTSMDLLKKIWELELINVLKISLIGHRKRILASLGDRLHDDPPQKPPRSITLREPSGNHTPP QLSPSLSQSTYTTGGSLDVPHIIMQGDARRRRNENYFDDIPRSKLERQMAQTGDWGEPSITLRPPNEATA STPVQYWQHHPEKLIFQSCDYKAFYLGSMLIKELRGTESTQDACAKMRANCQKSTEQMKKVPTIILSVSY KGVKFIDAANKNIIAEHEIRNISCAAQDPEDLSTFAYITKDLKSNHHYCHVFTAFDVNLAYEIILTLGQA FEVAYQLALQARKGGHSSTLPESFENKPSKPIPKPRVSIRKSVAPQTSCPNRLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001177397 |
ORF Size | 1212 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001177397.2 |
RefSeq Size | 7246 bp |
RefSeq ORF | 1215 bp |
Locus ID | 77531 |
UniProt ID | Q8BIZ1 |
Cytogenetics | 10 C2 |
MW | 45.9 kDa |
Gene Summary | Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211824 | Anks1b (untagged) - Mouse ankyrin repeat and sterile alpha motif domain containing 1B (Anks1b), transcript variant 4, (10ug) |
USD 503.00 |
|
MG219871 | Anks1b (tGFP-tagged) - Mouse ankyrin repeat and sterile alpha motif domain containing 1B (Anks1b) transcript variant 4, (10ug) |
USD 703.00 |
|
MR219871L3 | Lenti ORF clone of Anks1b (Myc-DDK-tagged) - Mouse ankyrin repeat and sterile alpha motif domain containing 1B (Anks1b), transcript variant 4 |
USD 803.00 |
|
MR219871L4 | Lenti ORF clone of Anks1b (mGFP-tagged) - Mouse ankyrin repeat and sterile alpha motif domain containing 1B (Anks1b), transcript variant 4 |
USD 803.00 |
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