Adamts3 (NM_177872) Mouse Tagged ORF Clone

CAT#: MR219542

Adamts3 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 3 (Adamts3), transcript variant 1

Product Images

Circular map for MR219542

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adamts3
• Synonyms: 1100001H14Rik; 6330442E02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219542 representing NM_177872
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTCCTGTCACTTTGGTTGATAGCAGCCTCTCTGGTAGAGGTTAGGACATCAGCTGATGGACAAG
  CTGGTACTGACGAAATGGTGCAAATAGATTTACCAACAAAGAGATACAGAGAGTATGAGCTGGTGACACC
  AGTTAGCACGAATCTAAAAGGACATTATCTTTCCCATATTCTTTCTGCAAATCACAAAAAGAGGTCACCC
  AGGGACGTGTCATCTAACTCAGAGCATTTGTTCTTCAACGTCACGGCATTTGGAAGAGATTTTCATCTTC
  GACTGAAGCCAAACACTCACTTCATAGCTCCTGGGGCTGTGGTGGAATGGCATGAGACAGCTCCAAGGCC
  TGGGAATACCACTGACCCCAGAGACAGCCATCTACACGGAAGTGCTTCAGAAGGAAGCTGGAGATCAGAG
  CCTTTGCAGACCAGCTGTGCCTATGTTGGTGACATCATGGACATTCCAGGAACCTCTGTTGCCATTAGCA
  ACTGTGATGGTCTGGCTGGGATGATAAAGAGCGATGATGAAGAGTATTTCATTGAACCCTTGGAGAGAGG
  GAAGCAGATGGATGAAGAAAATGGAAGGATTCACGTTGTCTACAAGAGATCAGCAGTGGAGCAACCTCCC
  AGAGATGTATCTGAAGACGTCTACCACAGGGAGTCGGACCTGGAAGGCCTTGACGATCTAGGTACAGTTT
  ACAGCAACATTGGCCAGCAGCTGAACAAGACAATGCGGCGCCGCAGACACACGGGAGAGAACGATTACAA
  CATCGAGGTGCTTCTGGGAGTGGATGACTCTGTGGTCCGTTTCCATGGCAAAGAGCATGTCCAAAATTAC
  CTCCTCACCCTGATGAACATTGTAAATGAAATCTACCACGATGAGTCCCTCGGAGTGCATATAAATGTGG
  TCCTGGTACGAATGATTATGCTTGGTTATGCAAAGTCCATCAGTCTCATCGAAAGGGGAAACCCGTCCAG
  GAGCCTGGAGAACGTGTGCCGATGGGCTTGCCAGCAACAGAAAACTGATCCCAACCACGCCGAACACCAT
  GATCACGCAATTTTCTTAACCAGGCAAGACTTTGGGCCTGCTGGAATGCAAGGATATGCTCCTGTCACTG
  GCATGTGTCACCCAGTGAGAAGTTGCACCCTGAATCATGAAGATGGTTTTTCATCTGCTTTTGTAGTAGC
  CCATGAAACTGGCCATGTGTTGGGCATGGAGCATGACGGGCAAGGCAATCGGTGTGGCGATGAGACCGCC
  ATGGGCAGTGTCATGGCGCCACTAGTGCAAGCTGCCTTCCATCGTTATCACTGGTCCAGATGCAGCGGTC
  AGGAACTGAAACGATACATCCATTCCTATGACTGCCTCCTTGATGACCCCTTTGAACATGACTGGCCCAA
  ACTCCCAGAACTTCCCGGAATTAATTATTCCATGGATGAGCAATGCCGTTTTGACTTTGGCGTTGGCTAC
  AAGATGTGTACTGCGTTCCGAACCTTTGACCCATGTAAACAGCTATGGTGCAGCCACCCTGATAACCCAT
  ACTTCTGTAAGACCAAGAAGGGGCCCCCACTCGACGGGACTGAGTGTGCTGCTGGCAAGTGGTGCTATAA
  AGGACACTGCATGTGGAAGAATGCTAACCAGCAAAAGCAAGATGGCAACTGGGGGTCGTGGACCAAATTT
  GGCTCCTGTTCTAGGACGTGTGGAACCGGTGTTCGTTTTAGAACACGCCAATGCAATAATCCCACGCCCA
  TCAATGGTGGGCAGGACTGCCCTGGTGTTAATTTTGAGTACCAGCTTTGTAATACAGAAGAATGCCAAAA
  GCACTTTGAGGACTTCAGGGCCCAACAGTGCCAACAGCGTAACTCACACTTTGACTACCAGAATACCAAA
  CACCACTGGCTGCCGCATGAACATCCCGACTCCAAGAAAAGATGCCATCTTTACTGTCAGTCCAAAGAGA
  CTGGGGATGTTGCTTACATGAAACAACTGGTGCACGACGGAACGCGATGCTCTTACAAAGATCCATACAG
  CATATGTGTGCGAGGAGAATGTGTGAAAGTGGGCTGTGATAGAGAAATCGGCTCCAACAAGGTTGAGGAT
  AAGTGTGGGGTCTGTGGAGGGGACAATTCCCACTGTCGAACTGTGAAAGGGACATTTACCAGGACACCCA
  GGAAACTTGGGAAGACGAGAGGCGCGTTCGTTTTACCCAAAGGAGCTCGTAATATTTCTCTTGCTGAAAC
  AAGAGAGACTAAAAATGTCCTGGCCATTAAGAATCAAGCCACAGGTCACTACATTTTAAATGGCAAAGGG
  GAGGAAGCCAAGTCACGGACCTTCATAGATCTTGGTGTGGAGTGGGATTACAACATTGAAGATGACATTG
  AAACCCTCCACACAGATGGCCCGCTACATGACCCCGTTATTGTTCTGATCATACCTCAGGAGAACGACAC
  GCGCTCCAGCCTGACGTATAAGTACATCATCCACGAAGACTCGGTGCCAACGATCAACAGCAACAACGTC
  ATCCAGGAGGAGCTGGATACCTTCGAGTGGGCGCTGAAGAGCTGGTCTCAGTGCTCTGAGCCCTGTGGCG
  GAGGATTCCAGTATACGAAATATGGATGCCGAAGGAAAAGCGACAGTAAAATGGTTCACCGCAGTTTCTG
  CGAGGTCAATAAAAAGCCAAAACCCATCAGACGCATGTGCAACATTCAGGAGTGCACACACCCGGTCTGG
  GCAGCGGATGAATGGGAGCACTGTAGCAGAAGCTGTGGCAGCTCCGGGAACCAGCTGCGCACCGTGCGCT
  GCCTCCAGCCACTGCAGGATGGCACCAACCGCTCGGTGCACAGCAAGTACTGCCTGGGAGACCGTCCTGA
  GAGCCGCCGGCCCTGTAACAGAGTGCCCTGTCCAGCTCAGTGGAAAACAGGATCCTGGAATGAGTGTTCA
  GTGACCTGCGGTGAGGGAACAGAGGTGAGACAGGTTCTGTGCAGGACTGGAGACCACTGTGATGGAGAAA
  AGCCCGAGTCTGTCAGACCCTGCCAGCTGCCTCCCTGTAATGATGAGCCATGTTTGGGTGACAAGTCCAT
  ATTCTGTCAAATGGAGGTGCTAGCAAGATACTGCTCCATACCAGGGTATAACAAGTTATGCTGCGAATCA
  TGTAGCAAGCGCAGTGGTACCCTGCCTCCGCCATACCTTCAAGAAGCTGCTGAAATCCACAGTGATGCTA
  TCTTTGGCCCTAGTGACCTCCCTAGGTCCCTAGTCATGCCTACACCCTTCCTCCCTTCCTCCTCAGGGAC
  CCGTGCTGAGAAGAAATCCTTGAGTAGCTTCTCTTCCATGGCAAACCCCAATGCGGATGCTGCCTTCAGA
  GCCAGCAGCAGAGCTAGTGCTGACTTCCCCCGCAAGAGAGCACATCAGGCTGGAAGAAAGACTCTGGAGT
  CAGTGCCATCCTCCTCATCCATAAAGACTGATCACCTTCGTCCCGCCTCCCCTGTGGCTGCTGCCCCCTC
  CCCTGCTATCAGCGATTCAATAGGTGCTTCTCCCCAGACAAGAACCCCAAAGAAAGATGATGCAAAGAGT
  GTTAGCAGGAGACATCCGACCAGGTCAACAGCCTCAGAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177872
• ORF Size: 3612 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177872.2, NP_808540.2
• RefSeq Size: 7582 bp
• RefSeq ORF: 3615 bp
• Locus ID: 330119
• Cytogenetics: 5 E1
• MW: 135.6 kDa