Trim16 (NM_053169) Mouse Tagged Lenti ORF Clone
CAT#: MR219315L4
- LentiORF®
Lenti ORF clone of Trim16 (mGFP-tagged) - Mouse tripartite motif-containing 16 (Trim16)
"NM_053169" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Trim16 |
Synonyms | 9130006M08Rik; AI482483; EBBP |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR219315).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053169 |
ORF Size | 1668 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053169.2, NP_444399.2 |
RefSeq Size | 3896 bp |
RefSeq ORF | 1671 bp |
Locus ID | 94092 |
UniProt ID | Q99PP9 |
Cytogenetics | 11 B2 |
Gene Summary | E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG219315 | Trim16 (tGFP-tagged) - Mouse tripartite motif-containing 16 (Trim16), (10ug) |
USD 718.00 |
|
MR219315 | Trim16 (Myc-DDK-tagged) - Mouse tripartite motif-containing 16 (Trim16) |
USD 518.00 |
|
MR219315L3 | Lenti ORF clone of Trim16 (Myc-DDK-tagged) - Mouse tripartite motif-containing 16 (Trim16) |
USD 818.00 |
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