Clmn (NM_053155) Mouse Tagged ORF Clone

CAT#: MR219220

Clmn (Myc-DDK-tagged) - Mouse calmin (Clmn), transcript variant 1

Product Images

Circular map for MR219220

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clmn
• Synonyms: 9330188N17Rik; AI428889; AI788815; mKIAA1188
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219220 representing NM_053155
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTCAGGAGTGGGACTGGTTCCAGCGCGAGGAGCTCATCGGGCAGATCAGCGACATCCGTGTGC
  AAAACCTGCAAGTGGAGAGGGAAAACGTGCAGAAGAGGACTTTCACACGATGGATAAACCTACACCTCGA
  AAAGTGTGACCCACCTCTAGAAGTTACGGACCTGTTTGTTGATATTCAAGATGGCAAAATCCTAATGGCT
  CTGTTAGAAGTCCTCTCTGGGCGAAATCTGCTGCATGAATACAAATCCTCATCACATCGTATTTTTCGGT
  TGAACAACATAGCGAAAGCACTCAAGTTTCTGGAAGATAGCAATGTCAAGCTGGTTAGCATCGATGCAGC
  GGAAATAGCGGACGGCAACCCCTCGCTGGTTCTCGGGCTGATATGGAACATCATTCTCTTCTTCCAGATA
  AAGGAGCTCACGGGCAACCTCAGCAGGAGTTCTCCATCTTCCAGCTTGTCACCGGGCTCTGGGGGCACCG
  ACTCCGACTCTTCCTACCCACCCACCCCCACCACCGAGAGGAGTGTGGCAGTGGCAGTGAAAGACCAGAG
  GAAGGCCATCAAGACCCTGCTGTCATGGGTGCAGAGGAAAACCAGGAAGTATGGTGTGGCAGTCCAAGAC
  TTTGCAGGCAGCTGGAGGAGTGGCCTGGCTTTCCTGGCTGTCATCAAAGCTATTGACCCCAGCCTTGTAG
  ACATGAAGCAGGCTCTGGAAGATTCCACCCGGGACAATCTGGAAAAGGCCTTCAGCATTGCCCATGATTC
  CCTGCATATCCCGAGGCTCCTGGAGCCGGAAGACATCATGGTTGACATGCCAGATGAGCAGTCCATTGTG
  ACATACGTGGCCCAGTTTCTAGAGCGCTTTCCAGAGTTGGAGCCTGAAGATTTTGTGAATCCGGACAAAG
  AAGCCCCCATCGAGTCCACCTTTGTCCGCATCAAAGAAAGCCCCTCTGAGCAGGGGAGCAGAGTCCTCCT
  CCTCAGCGAGAACGGAGAGCGGGCCTACACTGTCAACCAGGAAACCAGCTACCCGCCGCCTGACAAGGTC
  TTCGTCTGTGACCAGCTTGAGAGTCCGACTGGCTTCTGTCTGGATAGTGCTCCCAGCCACAAACTGTCAG
  ACAGCTCCACAGAGTTTATGCATGAGATTATTGACCAAGTCCTCCAGGGGAGCACGGGGAAGACTGGCTC
  CATCGCAGAGCCAACTCCAGAGTCGTCCATTTTGTCAACCAGAAAGGACGGCAGGAGGTCCAACTCTTTG
  CCTGTCAAGAAAACCGTCCACTTCGAGGCAGACTTGCACAAGGATGCCTCGTGTAGTAAGGACCCTTTCT
  ATAGCTCTGACTTCCGCTTCGAGGGGAGCCCCAAGGCAACCAAGGAGCTGTCAAAGCAGGACGGCCATGT
  CTCACTGGCTGAGGTGTCCAAGGAAAAGAAGAAGTCAGAACAGGAAGCCAGGCTAGTGCTGGAAGCTGCC
  TCAGACAAGGTCCCCGAAAGCACGGTCGATGGTCTGGATGCTGTGCCCCAAGATGCTCAGCCTTCCCAGG
  ACTCCTCCTTCTGTAACGGCACTGTAGAGAGCCCATCCAGCCAAGGTGAGAAAGGCCCTCCTCCATCATC
  CCCTGGAGATCATACTCTCCTGGCCAACTCCACTGAGCTCAAGGTTCAGCTGCTGACCGTTGAGCCTATG
  GACAAGGAAGACTACTTTGAATGCATCCCACTAAAAGCTTCAAAGTTTAACAGGGACCTAGTGGATTTCG
  CCTCCACCAGCCAGGCTTTTGGTGAGGATCCTTCGTCCCACGAGAAGACCCGTGGGGAGGAAGAGGGTTC
  TGAGAATCACGCTGAGAAACCAGGGAAAAGGAAATCAAAGTCTCCCCGTGCAGAAACAGAGGCCGCCGAG
  TCCAGATTGGAGCCCAAGAAGCTCGAGCCTCCTCCCAAGGATCCCGAACAAGAAGACCAAGGCCACGCTT
  TGCCCCCGGAAACCCCTGCAGACAAAAAGCCCAAAGTGTATGAAAAGGCCAAGAGAAAGTCCACTCGTCA
  TCACAGCGAGGAAGAAGGAGAAGCAGAAAGTGGCTTCTCTGCAGTCTGTGAAGAAGAAATACCATCCGCC
  CCTCCCAGCACCAGCGTCAGCCTGGAAACCCTCAGGAGTCACAGCGAAGAAGGTTTGGATTTCAAACCTT
  CGCCGCCGCTCTCGAAGATCTCTGTCATCCCCCACGACCTGTTCTACTACCCTCACTACGAAGTGCCCCT
  GGCCGCTGTGCTGGAGGCTTACGCGGAAGGCGGGGAGGATTTGAAAAGTGAAGACACAGACCTAGAGCAT
  CCAGAGGACAGCTATCTGCAGGACTCCAGGGAGGAGGAGGCCGACGAGGACGAGGAGGAGGCCCAGAGCT
  CTCAAAGCAGCTGCAGTTTCTCCTTGCCTGTTGACAACAGCTACCCCAGCGTCAGTGAGCATGTGTCCCA
  TGTCGATGGGAGTTCTGAGGGACCCACGTCAGCCTTGGGACCTGGCTCTCCACCCTCTCATGAGGACCAC
  CAGCCAAAGGAAACCAAAGAGAATGGCCCTGTGGAAAGCCAGCAGTCCCAAGAACCCCCAAACCCGGAAC
  TGCCCACAAAGCCATTAGAAGAAAAGTTAACGGAAGCCTCAACTAGCAGTAAAAAGAAGGAAAAACGGAA
  ACACATGGACCACGTAGAAAGCTCATTATTTATAGCCCCTGGGACGGTCCGCTCCTCAGACGACCTTGAA
  GAAAACTCCAGCGAACACAAGGTCCCTTCCAGGAATAGTCACAGTGACTCCAGCATTTACATTCGGCGAC
  ACACTAATAGGTCTTTGGAATTGGACCATTTTAGCTATGTTCAATTGAGGAACGCAGCTGATCTGGATGA
  CAGGAGAAACAGAGTGTTAAACAGGTACAATTCTCAGAAGCTCACTGAGCTGATTTTACAGTTCTACGGC
  ATCAGAGCAGACATGAAGCGCGAGTACAAACATGCCAGGCTGTCTATGACTGGCACCAACTCCTCTGGAG
  AAGCTGTGCCCCTGGGGAACCAGAGCCCGCCCAACGACTCTCTGACGCAGTTTGTACAGCAGCCCGACGT
  GATATATTTCATTCTCTTCCTCTGGCTCCTCGTTTACTGCTTACTGCTCTTCCCACAGCTTGATGTCAGC
  CGGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219220 representing NM_053155
  Red=Cloning site Green=Tags(s)
  
  MAAQEWDWFQREELIGQISDIRVQNLQVERENVQKRTFTRWINLHLEKCDPPLEVTDLFVDIQDGKILMA
  LLEVLSGRNLLHEYKSSSHRIFRLNNIAKALKFLEDSNVKLVSIDAAEIADGNPSLVLGLIWNIILFFQI
  KELTGNLSRSSPSSSLSPGSGGTDSDSSYPPTPTTERSVAVAVKDQRKAIKTLLSWVQRKTRKYGVAVQD
  FAGSWRSGLAFLAVIKAIDPSLVDMKQALEDSTRDNLEKAFSIAHDSLHIPRLLEPEDIMVDMPDEQSIV
  TYVAQFLERFPELEPEDFVNPDKEAPIESTFVRIKESPSEQGSRVLLLSENGERAYTVNQETSYPPPDKV
  FVCDQLESPTGFCLDSAPSHKLSDSSTEFMHEIIDQVLQGSTGKTGSIAEPTPESSILSTRKDGRRSNSL
  PVKKTVHFEADLHKDASCSKDPFYSSDFRFEGSPKATKELSKQDGHVSLAEVSKEKKKSEQEARLVLEAA
  SDKVPESTVDGLDAVPQDAQPSQDSSFCNGTVESPSSQGEKGPPPSSPGDHTLLANSTELKVQLLTVEPM
  DKEDYFECIPLKASKFNRDLVDFASTSQAFGEDPSSHEKTRGEEEGSENHAEKPGKRKSKSPRAETEAAE
  SRLEPKKLEPPPKDPEQEDQGHALPPETPADKKPKVYEKAKRKSTRHHSEEEGEAESGFSAVCEEEIPSA
  PPSTSVSLETLRSHSEEGLDFKPSPPLSKISVIPHDLFYYPHYEVPLAAVLEAYAEGGEDLKSEDTDLEH
  PEDSYLQDSREEEADEDEEEAQSSQSSCSFSLPVDNSYPSVSEHVSHVDGSSEGPTSALGPGSPPSHEDH
  QPKETKENGPVESQQSQEPPNPELPTKPLEEKLTEASTSSKKKEKRKHMDHVESSLFIAPGTVRSSDDLE
  ENSSEHKVPSRNSHSDSSIYIRRHTNRSLELDHFSYVQLRNAADLDDRRNRVLNRYNSQKLTELILQFYG
  IRADMKREYKHARLSMTGTNSSGEAVPLGNQSPPNDSLTQFVQQPDVIYFILFLWLLVYCLLLFPQLDVS
  RL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053155
• ORF Size: 3156 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053155.2, NP_444385.2
• RefSeq Size: 11962 bp
• RefSeq ORF: 3159 bp
• Locus ID: 94040
• UniProt ID: Q8C5W0
• Cytogenetics: 12 E
• MW: 117.7 kDa