Klk1b27 (NM_020268) Mouse Tagged ORF Clone

CAT#: MR218780

Klk1b27 (Myc-DDK-tagged) - Mouse kallikrein 1-related peptidase b27 (Klk1b27)

Product Images

Circular map for MR218780

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Klk1b27
• Synonyms: Gk27; Klk2; Klk21l; Klk27; mGK-27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218780 representing NM_020268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTTCCTGATCCTGTTCCTTGCCCTGTCCCTAGGAGGGATTGATGCTGCGCCTCCTGTCCAGTCTA
  GAATAATTGGAGGATTTAAATGTAAGAAGAATTCTCAACCCTGGCATGTGGCTGTGCTCCGCTCCAACAA
  ATATATATGCGGGGGAGTCCTGTTGGACCCCAACTGGGTTCTCACAGCTGCCCACTGCTATGGCAATGAC
  ACCAGCCAGCATAATGTTTGGCTGGGCAAAAACAAGCTATTCCAACGTGAACCCTCTGCTCAGCACCGAT
  GGGTCAGCAAAAGCTTCCCCCACCCTGACTACAACATGAGCCTCCTGAATGACCACATCCCACATCCTGA
  GGACAAGAGCAATGACCTGATGCTGCTCCGTCTCAGCAAGCCTGCTGACATCACAGATGCTGTGAAGCCC
  ATCGACCTGCCCACTGAGGAGCCCAAGCTGGGGAGCACATGCCTAGCCTCAGGCTGGGGCAGCATTACAC
  CCACAAAATACCAAATCCCAAATGATCTTCAGTGTGTGTTCATCAAGCTCCTGCCCAATGAGAACTGTGC
  CAAAGCCTACGTACATAAAGTCACAGATGTCATGCTGTGTGTAGGAGAGACGGGTGGAGGCAAAGGCACT
  TGCAAGGGTGACTCAGGAGGCCCACTGATCTGTGATGGTGTTCTCCACGGTATCACATCATGGGGCTCTA
  TCCCATGCGCTAAACCCAATGCGCCAGGCGTCTTCACAAAACTTATTAAGTTTACCTCCTGGATAAAAGA
  CACTATGGCAAAAAACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218780 representing NM_020268
  Red=Cloning site Green=Tags(s)
  
  MRFLILFLALSLGGIDAAPPVQSRIIGGFKCKKNSQPWHVAVLRSNKYICGGVLLDPNWVLTAAHCYGND
  TSQHNVWLGKNKLFQREPSAQHRWVSKSFPHPDYNMSLLNDHIPHPEDKSNDLMLLRLSKPADITDAVKP
  IDLPTEEPKLGSTCLASGWGSITPTKYQIPNDLQCVFIKLLPNENCAKAYVHKVTDVMLCVGETGGGKGT
  CKGDSGGPLICDGVLHGITSWGSIPCAKPNAPGVFTKLIKFTSWIKDTMAKNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020268
• ORF Size: 792 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020268.3, NP_064664.1
• RefSeq Size: 889 bp
• RefSeq ORF: 792 bp
• Locus ID: 16619
• UniProt ID: Q9JM71
• Cytogenetics: 7 28.54 cM
• MW: 28.7 kDa
• Gene Summary: This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic cleavage of the activation peptide to generate the functional enzyme. This gene is located in a cluster of several related kallikrein genes on chromosome 7. [provided by RefSeq, Feb 2016]