Zp1 (NM_009580) Mouse Tagged ORF Clone

CAT#: MR218554

Zp1 (Myc-DDK-tagged) - Mouse zona pellucida glycoprotein 1 (Zp1)

Product Images

Circular map for MR218554

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218554 representing NM_009580
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGGGGTTGTTTTGTGGTCCTGCTTCTGCTGGCGGCAGCTCCCCTGAGATTGGGTCAGCGCCTGC
  ATCTCGAGCCTGGCTTTGAATACAGCTATGACTGTGGGGTACGGGGCATGCAGCTGCTGGTGTTCCCTAG
  GCCAAACCAGACTGTCCAATTCAAGGTGCTGGATGAATTTGGGAACCGGTTTGAGGTGAATAACTGCTCT
  ATCTGCTACCACTGGGTGACCAGTGAGGCCCAGGAGCACACAGTATTCTCAGCTGATTACAAAGGCTGCC
  ACGTTCTAGAGAAGGATGGGCGGTTCCACCTGAGGGTGTTCATACAAGCCGTCCTACCCAATGGCCGTGT
  GGATATAGCACAAGATGTCACACTGATCTGTCCCAAACCAGACCACACCGTGACTCCGGACCCCTACCTG
  GCTCCACCCACCACACCTGAGCCTTTTACACCTCATGCTTTTGCCCTTCACCCCATCCCTGACCACACCT
  TGGCTGGGTCTGGCCACACTGGTCTCACTACGTTGTACCCAGAACAGAGCTTCATCCATCCAACTCCTGC
  TCCGCCATCCCTAGGACCTGGACCTGCTGGATCCACTGTACCTCACTCCCAATGGGGCACGCTGGAGCCC
  TGGGAATTGACTGAGCTGGATTCTGTAGGTACCCATCTGCCCCAGGAGCGGTGCCAGGTAGCCTCCGGGC
  ACATCCCATGCATGGTAAATGGAAGTTCCAAGGAAACCTGTCAGCAGGCTGGCTGCTGCTATGACAGTAC
  CAAAGAAGAGCCCTGTTACTATGGCAACACAGTCACTCTCCAGTGTTTCAAAAGTGGCTACTTTACCTTG
  GTCATGTCACAAGAAACAGCCTTGACACATGGAGTCCTGCTAGACAATGTCCACCTGGCCTATGCCCCCA
  ACGGATGCCCCCCTACCCAGAAGACAAGTGCTTTTGTGGTCTTCCATGTCCCTCTCACCCTCTGTGGAAC
  AGCAATCCAGGTGGTCGGCGAACAACTCATCTATGAGAACCAGCTGGTGTCTGACATTGATGTCCAAAAG
  GGGCCACAGGGTTCCATCACTCGGGACAGTGCCTTCCGGCTTCATGTTCGCTGCATCTTCAATGCTAGCG
  ATTTCCTGCCCATCCAGGCATCTATCTTCTCACCCCAACCACCTGCCCCTGTGACTCAATCTGGACCCCT
  GAGGCTGGAGCTGAGGATTGCCACGGATAAGACTTTCAGCTCCTACTATCAGGGGAGTGACTATCCCCTT
  GTGAGACTGCTCCGGGAACCAGTCTATGTAGAGGTCCGACTCCTGCAGAGAACTGATCCCAGTCTGGTTC
  TGGTGCTACACCAGTGCTGGGCCACGCCCACCACCAGCCCCTTTGAGCAGCCCCAATGGCCTATTCTGTC
  AGATGGGTGTCCTTTCAAGGGTGACAACTACAGAACACAAGTAGTGGCCGCAGACAGGGAGGCGCTTCCC
  TTCTGGTCCCACTATCAGCGCTTCACCATCACCACCTTCATGCTCCTTGACTCCAGCTCCCAGAATGCCC
  TTAGGGGACAGGTCTATTTCTTCTGTAGTGCCTCTGCCTGCCACCCTCTGGGGTCTGATACATGCTCTAC
  TACGTGTGACTCTGGGATAGCAAGGCGTCGACGATCCTCTGGTCACCACAACATCACTCTCCGGGCCCTG
  GATATTGTAAGCTCTCCAGGGGCAGTGGGCTTTGAGGATGCTGCTAAACTTGAGCCCTCAGGTTCCAGCA
  GGAACTCCAGCTCAAGAATGCTGCTCCTGCTGCTGGCCATCACCCTAGCCCTGGCGGCCGGGATCTTTGT
  GGGTCTGATCTGGGCCTGGGCCCAGAAGCTCTGGGAAGGCATCAGATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218554 representing NM_009580
  Red=Cloning site Green=Tags(s)
  
  MAWGCFVVLLLLAAAPLRLGQRLHLEPGFEYSYDCGVRGMQLLVFPRPNQTVQFKVLDEFGNRFEVNNCS
  ICYHWVTSEAQEHTVFSADYKGCHVLEKDGRFHLRVFIQAVLPNGRVDIAQDVTLICPKPDHTVTPDPYL
  APPTTPEPFTPHAFALHPIPDHTLAGSGHTGLTTLYPEQSFIHPTPAPPSLGPGPAGSTVPHSQWGTLEP
  WELTELDSVGTHLPQERCQVASGHIPCMVNGSSKETCQQAGCCYDSTKEEPCYYGNTVTLQCFKSGYFTL
  VMSQETALTHGVLLDNVHLAYAPNGCPPTQKTSAFVVFHVPLTLCGTAIQVVGEQLIYENQLVSDIDVQK
  GPQGSITRDSAFRLHVRCIFNASDFLPIQASIFSPQPPAPVTQSGPLRLELRIATDKTFSSYYQGSDYPL
  VRLLREPVYVEVRLLQRTDPSLVLVLHQCWATPTTSPFEQPQWPILSDGCPFKGDNYRTQVVAADREALP
  FWSHYQRFTITTFMLLDSSSQNALRGQVYFFCSASACHPLGSDTCSTTCDSGIARRRRSSGHHNITLRAL
  DIVSSPGAVGFEDAAKLEPSGSSRNSSSRMLLLLLAITLALAAGIFVGLIWAWAQKLWEGIRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009580
• ORF Size: 1869 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009580.2, NP_033606.2
• RefSeq Size: 1925 bp
• RefSeq ORF: 1872 bp
• Locus ID: 22786
• UniProt ID: Q62005
• Cytogenetics: 19 7.36 cM
• MW: 69.2 kDa
• Gene Summary: The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.[UniProtKB/Swiss-Prot Function]