Zp1 (NM_009580) Mouse Tagged ORF Clone
CAT#: MR218554
- TrueORF®
Zp1 (Myc-DDK-tagged) - Mouse zona pellucida glycoprotein 1 (Zp1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_009580" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Zp1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218554 representing NM_009580
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGGGGTTGTTTTGTGGTCCTGCTTCTGCTGGCGGCAGCTCCCCTGAGATTGGGTCAGCGCCTGC ATCTCGAGCCTGGCTTTGAATACAGCTATGACTGTGGGGTACGGGGCATGCAGCTGCTGGTGTTCCCTAG GCCAAACCAGACTGTCCAATTCAAGGTGCTGGATGAATTTGGGAACCGGTTTGAGGTGAATAACTGCTCT ATCTGCTACCACTGGGTGACCAGTGAGGCCCAGGAGCACACAGTATTCTCAGCTGATTACAAAGGCTGCC ACGTTCTAGAGAAGGATGGGCGGTTCCACCTGAGGGTGTTCATACAAGCCGTCCTACCCAATGGCCGTGT GGATATAGCACAAGATGTCACACTGATCTGTCCCAAACCAGACCACACCGTGACTCCGGACCCCTACCTG GCTCCACCCACCACACCTGAGCCTTTTACACCTCATGCTTTTGCCCTTCACCCCATCCCTGACCACACCT TGGCTGGGTCTGGCCACACTGGTCTCACTACGTTGTACCCAGAACAGAGCTTCATCCATCCAACTCCTGC TCCGCCATCCCTAGGACCTGGACCTGCTGGATCCACTGTACCTCACTCCCAATGGGGCACGCTGGAGCCC TGGGAATTGACTGAGCTGGATTCTGTAGGTACCCATCTGCCCCAGGAGCGGTGCCAGGTAGCCTCCGGGC ACATCCCATGCATGGTAAATGGAAGTTCCAAGGAAACCTGTCAGCAGGCTGGCTGCTGCTATGACAGTAC CAAAGAAGAGCCCTGTTACTATGGCAACACAGTCACTCTCCAGTGTTTCAAAAGTGGCTACTTTACCTTG GTCATGTCACAAGAAACAGCCTTGACACATGGAGTCCTGCTAGACAATGTCCACCTGGCCTATGCCCCCA ACGGATGCCCCCCTACCCAGAAGACAAGTGCTTTTGTGGTCTTCCATGTCCCTCTCACCCTCTGTGGAAC AGCAATCCAGGTGGTCGGCGAACAACTCATCTATGAGAACCAGCTGGTGTCTGACATTGATGTCCAAAAG GGGCCACAGGGTTCCATCACTCGGGACAGTGCCTTCCGGCTTCATGTTCGCTGCATCTTCAATGCTAGCG ATTTCCTGCCCATCCAGGCATCTATCTTCTCACCCCAACCACCTGCCCCTGTGACTCAATCTGGACCCCT GAGGCTGGAGCTGAGGATTGCCACGGATAAGACTTTCAGCTCCTACTATCAGGGGAGTGACTATCCCCTT GTGAGACTGCTCCGGGAACCAGTCTATGTAGAGGTCCGACTCCTGCAGAGAACTGATCCCAGTCTGGTTC TGGTGCTACACCAGTGCTGGGCCACGCCCACCACCAGCCCCTTTGAGCAGCCCCAATGGCCTATTCTGTC AGATGGGTGTCCTTTCAAGGGTGACAACTACAGAACACAAGTAGTGGCCGCAGACAGGGAGGCGCTTCCC TTCTGGTCCCACTATCAGCGCTTCACCATCACCACCTTCATGCTCCTTGACTCCAGCTCCCAGAATGCCC TTAGGGGACAGGTCTATTTCTTCTGTAGTGCCTCTGCCTGCCACCCTCTGGGGTCTGATACATGCTCTAC TACGTGTGACTCTGGGATAGCAAGGCGTCGACGATCCTCTGGTCACCACAACATCACTCTCCGGGCCCTG GATATTGTAAGCTCTCCAGGGGCAGTGGGCTTTGAGGATGCTGCTAAACTTGAGCCCTCAGGTTCCAGCA GGAACTCCAGCTCAAGAATGCTGCTCCTGCTGCTGGCCATCACCCTAGCCCTGGCGGCCGGGATCTTTGT GGGTCTGATCTGGGCCTGGGCCCAGAAGCTCTGGGAAGGCATCAGATAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218554 representing NM_009580
Red=Cloning site Green=Tags(s) MAWGCFVVLLLLAAAPLRLGQRLHLEPGFEYSYDCGVRGMQLLVFPRPNQTVQFKVLDEFGNRFEVNNCS ICYHWVTSEAQEHTVFSADYKGCHVLEKDGRFHLRVFIQAVLPNGRVDIAQDVTLICPKPDHTVTPDPYL APPTTPEPFTPHAFALHPIPDHTLAGSGHTGLTTLYPEQSFIHPTPAPPSLGPGPAGSTVPHSQWGTLEP WELTELDSVGTHLPQERCQVASGHIPCMVNGSSKETCQQAGCCYDSTKEEPCYYGNTVTLQCFKSGYFTL VMSQETALTHGVLLDNVHLAYAPNGCPPTQKTSAFVVFHVPLTLCGTAIQVVGEQLIYENQLVSDIDVQK GPQGSITRDSAFRLHVRCIFNASDFLPIQASIFSPQPPAPVTQSGPLRLELRIATDKTFSSYYQGSDYPL VRLLREPVYVEVRLLQRTDPSLVLVLHQCWATPTTSPFEQPQWPILSDGCPFKGDNYRTQVVAADREALP FWSHYQRFTITTFMLLDSSSQNALRGQVYFFCSASACHPLGSDTCSTTCDSGIARRRRSSGHHNITLRAL DIVSSPGAVGFEDAAKLEPSGSSRNSSSRMLLLLLAITLALAAGIFVGLIWAWAQKLWEGIRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009580 |
ORF Size | 1869 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009580.2, NP_033606.2 |
RefSeq Size | 1925 bp |
RefSeq ORF | 1872 bp |
Locus ID | 22786 |
UniProt ID | Q62005 |
Cytogenetics | 19 7.36 cM |
MW | 69.2 kDa |
Gene Summary | The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219813 | Zp1 (untagged) - Mouse zona pellucida glycoprotein 1 (Zp1), (10ug) |
USD 639.00 |
|
MG218554 | Zp1 (tGFP-tagged) - Mouse zona pellucida glycoprotein 1 (Zp1), (10ug) |
USD 780.00 |
|
MR218554L3 | Lenti ORF clone of Zp1 (Myc-DDK-tagged) - Mouse zona pellucida glycoprotein 1 (Zp1) |
USD 880.00 |
|
MR218554L4 | Lenti ORF clone of Zp1 (mGFP-tagged) - Mouse zona pellucida glycoprotein 1 (Zp1) |
USD 880.00 |
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