Sgcz (NM_145841) Mouse Tagged ORF Clone

CAT#: MR218325

Sgcz (Myc-DDK-tagged) - Mouse sarcoglycan zeta (Sgcz)

Product Images

Circular map for MR218325

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sgcz
• Synonyms: C230085N17Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218325 representing NM_145841
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGATCCACGGACCTGGACATCCAGGAGCTCAAGATGACTCGAGAACAATACATACTAGCCACAC
  AGCAGAACAACCTGCCGAGACCTGAAAACGCACAACTCTACCCTGTGGGGATTTATGGATGGCGAAAGAG
  GTGCTTATACTTCTTTGTCCTTCTGCTGTTGGTTACCATGATAGTTAACTTAGCCATGACAATATGGATA
  TTGAAGGTTATGAATTTCACTGTGGATGGTATGGGGAATCTGAGAGTCACCAAGAAGGGAATCCGACTTG
  AAGGTATATCTGAGTTTCTACTACCATTGTATGTGAAAGAAATTCATTCCCGAAAGGACAGTCCATTGGT
  CTTACAGTCTGACAGAAATGTAACAGTGAATGCAAGGAACCATATGGGGCAGCTCACTGGACAGCTGACA
  GTCGGAGCTGAAGCTGTTGAGGCACAGTGCAAAAGATTCGAAGTGAGAGCAAGTGAAGATGGCAGGGTGC
  TGTTTTCTGCAGATGAAGATGAGATCACTATTGGGGCTGAGAAGCTAAAGGTTACAGGAACTGAAGGAGC
  AGTATTTGGACACTCTGTGGAAACACCTCACATCAGAGCAGAGCCATCTCAAGACCTCAGGCTTGAATCA
  CCAACCAGATCCTTGAAAATGGAGGCTCCACGTGGGGTCCAGGTGAGCGCAGCTGCAGGTGACTTCAAGG
  CTACCTGCAGGAAGGAGCTGCACTTACAGTCCACAGAAGGGGAGATATTTTTAAATGCAGATTCAATCCG
  GCTGGGAAATCTACCAATCGGTTCCTTCTCTTCTTCAACCAGCTCCTCAAATTCTCGACAGACAGTGTAT
  GAACTGTGCGTCTGCCCCAATGGCAAACTCTACCTTTCTCCAGCTGGGGTAGGCTCCACCTGTCAGTCCA
  GTAGTAGCATTTGCTTGTGGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218325 representing NM_145841
  Red=Cloning site Green=Tags(s)
  
  MDRSTDLDIQELKMTREQYILATQQNNLPRPENAQLYPVGIYGWRKRCLYFFVLLLLVTMIVNLAMTIWI
  LKVMNFTVDGMGNLRVTKKGIRLEGISEFLLPLYVKEIHSRKDSPLVLQSDRNVTVNARNHMGQLTGQLT
  VGAEAVEAQCKRFEVRASEDGRVLFSADEDEITIGAEKLKVTGTEGAVFGHSVETPHIRAEPSQDLRLES
  PTRSLKMEAPRGVQVSAAAGDFKATCRKELHLQSTEGEIFLNADSIRLGNLPIGSFSSSTSSSNSRQTVY
  ELCVCPNGKLYLSPAGVGSTCQSSSSICLWN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145841
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145841.2, NP_665840.2
• RefSeq Size: 2389 bp
• RefSeq ORF: 936 bp
• Locus ID: 244431
• UniProt ID: Q8BX51
• Cytogenetics: 8 A4
• MW: 34.9 kDa
• Gene Summary: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. May play a role in the maintenance of striated muscle membrane stability.[UniProtKB/Swiss-Prot Function]