Eef1akmt4-ece2 (NM_177940) Mouse Tagged ORF Clone

CAT#: MR218231

Ece2 (Myc-DDK-tagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 2

Product Images

Circular map for MR218231

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eef1akmt4-ece2
• Synonyms: 1810009K13Rik; 6330509A19Rik; 9630025D12Rik; BB127715; Ece-2; Eef1akmt4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218231 representing NM_177940
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCTCCCCGGACTCCGGTGTCCCCTCCGGAGTTACCGGAGAAGAACTTCCAGTACCGCCAAGTCC
  AGTACTGGGACCAGCGCTACAAAGACGCGGCCGACTCTGGACCCTACGAGTGGTTCGGAGACTTCGCTTC
  CTTCCGTGCCCTCCTAGAGCCGGAGCTGTGTCCTGAGGACCGTATCCTCGTGCTAGGTTGCGGGAACAGT
  GCCTTGAGCTATGAGCTGTTCCTTGGAGGCTTCCCTAATGTGACCAGTGTGGATTACTCTCCAGTAGTGG
  TGGCAGCCATGCAAGTTCGCTATGCCCATGTACCCAGTCTACGCTGGGAGACCATGGATGTTCGAGCACT
  GGACTTCCCTAGTGGGTCTTTTGATGTTGTCCTCGAGAAGGGTACTCTGGATGCCATGCTGGCTGGGGAG
  CCAGATCCCTGGAATGTATCTTCTGAAGGTGTCCACACTGTGGACCAGGTGCTGAGTGAGGTGGGATTCC
  AGAAGAGGACAAGACAACTGTTTGGTTCACACACACAGTTGGAGCTGGTCTTGGCAGGCCTCATTCTAGT
  GTTGGCTGCCCTTCTTTTGGGCTGCCTCGTGGCTCTGTGGGTCCACAGAGACCCAGCCCATAGCACCTGC
  GTCACAGAAGCCTGCATTCGAGTAGCTGGAAAAATCCTGGAGTCTCTAGACCGTGGGGTGAGCCCCTGTC
  AGGACTTTTACCAGTTCTCCTGTGGAGGCTGGATTCGAAGAAACCCTCTACCCAATGGACGTTCTCGCTG
  GAACACCTTCAACAGCCTCTGGGACCAGAACCAGGCCATACTGAAGCACCTACTTGAGAACACCACTTTC
  AATTCCAGCAGTGAAGCTGAGAGGAAGACTCGGAGTTTCTACCTGTCCTGCCTACAGTCGGAGCGCATTG
  AGAAGCTAGGAGCCAAGCCACTTAGAGACCTCATTGACAAGATCGGTGGTTGGAACATAACGGGGCCTTG
  GGACGAGGACAGCTTCATGGATGTGCTCAAGGCAGTCGCAGGGACCTACAGAGCCACCCCCTTCTTCACC
  GTCTACGTCAGTGCTGATTCTAAGAGTTCTAACAGCAATATCATCCAGGTGGACCAGTCTGGGCTTTTTC
  TGCCCTCTCGAGATTACTACCTAAATAGAACTGCCAATGAGAAAGTTCTCACTGCCTACCTGGACTACAT
  GGTGGAGCTGGGAGTGCTGCTGGGTGGACAGCCGACCTCCACTCGGGAGCAGATGCAGCAGGTGCTGGAG
  CTGGAGATACAGCTGGCTAACATCACTGTGCCCCAGGACCAGCGGCGTGATGAGGAGAAGATCTATCACA
  AGATGAGCATCTCAGAGCTGCAGGCTCTCGCGCCCGCCGTGGACTGGCTGGAGTTCCTTTCTTTCTTGTT
  ATCGCCACTTGAGTTGGGTGATTCTGAGCCTGTGGTGGTGTATGGGACTGAGTATTTACAGCAGGTGTCG
  GAGCTCATCAACCGTACTGAACCAAGCATCCTGAACAATTACCTAATTTGGAACCTGGTACAGAAGACGA
  CCTCAAGCCTTGACCAGCGCTTTGAGACTGCACAGGAGAAACTGCTGGAGACCCTCTACGGTACCAAGAA
  GTCCTGCACTCCGAGGTGGCAGACCTGCATCTCCAATACAGATGATGCCCTTGGCTTTGCTCTGGGTTCA
  CTCTTTGTGAAAGCCACATTTGACCGACAAAGCAAGGAAATCGCCGAGGGGATGATCAATGAAATCCGCT
  CTGCTTTTGAGGAGACCCTGGGAGACTTGGTTTGGATGGATGAGAAGACCCGGCTGGCAGCCAAGGAGAA
  AGCAGATGCCATCTATGATATGATTGGTTTCCCTGATTTCATCCTGGAGCCCAAAGAGCTGGATGATGTT
  TATGATGGGTATGAAGTCTCTGAAGATTCTTTTTTCCAAAACATGTTGAATCTGTACAACTTCTCAGCTA
  AGGTGATGGCTGACCAGCTCCGCAAACCTCCCAGCCGAGACCAGTGGAGCATGACACCTCAGACCGTGAA
  CGCTTACTACCTTCCAACCAAGAATGAAATCGTCTTCCCTGCTGGCATCTTGCAGGCCCCCTTCTATGCT
  CACAACCATCCAAAGGCCTTGAACTTTGGTGGCATCGGCGTAGTGATGGGCCATGAGTTGACACATGCCT
  TTGATGACCAAGGGCGTGAGTATGACAAAGAAGGGAATCTGCGTCCTTGGTGGCAGAATGAGTCACTGAC
  GGCTTTCCAGAACCATACAGCCTGCATGGAAGAACAGTACAGCCAGTACCAGGTCAATGGAGAGAGGCTC
  AATGGACTCCAGACCCTGGGGGAAAACATCGCCGATAATGGGGGCCTTAAGGCTGCTTACAATGCTTACA
  AAGCATGGCTGAGAAAGCATGGGGAGGAGCAGCCGCTGCCTGCTGTGGGGCTCACCAATCACCAGCTTTT
  CTTCGTGGGATTTGCTCAGGTGTGGTGCTCGGTCCGCACACCAGAGAGCTCTCACGAGGGGCTGGTGACC
  GACCCCCACAGCCCTGCCCGTTTCCGAGTGCTGGGCACTCTCTCCAACTCCCGAGACTTCCTTCGGCACT
  TCGGCTGCCCTGTCGGCTCCCCCATGAACCCAGGGCAGCTATGTGAGGTGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218231 representing NM_177940
  Red=Cloning site Green=Tags(s)
  
  MASPRTPVSPPELPEKNFQYRQVQYWDQRYKDAADSGPYEWFGDFASFRALLEPELCPEDRILVLGCGNS
  ALSYELFLGGFPNVTSVDYSPVVVAAMQVRYAHVPSLRWETMDVRALDFPSGSFDVVLEKGTLDAMLAGE
  PDPWNVSSEGVHTVDQVLSEVGFQKRTRQLFGSHTQLELVLAGLILVLAALLLGCLVALWVHRDPAHSTC
  VTEACIRVAGKILESLDRGVSPCQDFYQFSCGGWIRRNPLPNGRSRWNTFNSLWDQNQAILKHLLENTTF
  NSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGWNITGPWDEDSFMDVLKAVAGTYRATPFFT
  VYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMVELGVLLGGQPTSTREQMQQVLE
  LEIQLANITVPQDQRRDEEKIYHKMSISELQALAPAVDWLEFLSFLLSPLELGDSEPVVVYGTEYLQQVS
  ELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISNTDDALGFALGS
  LFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTRLAAKEKADAIYDMIGFPDFILEPKELDDV
  YDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYA
  HNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLTAFQNHTACMEEQYSQYQVNGERL
  NGLQTLGENIADNGGLKAAYNAYKAWLRKHGEEQPLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVT
  DPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177940
• ORF Size: 2643 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177940.1, NP_808809.1
• RefSeq Size: 3349 bp
• RefSeq ORF: 2646 bp
• Locus ID: 110599584
• UniProt ID: P0DPE0
• Cytogenetics: 16 B1
• MW: 99.9 kDa
• Gene Summary: This locus represents naturally occurring readthrough transcription between the adjacent genes eukaryotic translation elongation factor 1 alpha lysine specific methyltransferase 4 (GeneID:110599566) and endothelin converting enzyme 2 (GeneID:107522). The readthrough transcript representing this gene encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Jul 2017]