Elf4 (NM_019680) Mouse Tagged ORF Clone

CAT#: MR217932

Elf4 (Myc-DDK-tagged) - Mouse E74-like factor 4 (ets domain transcription factor) (Elf4)

Product Images

Circular map for MR217932

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Elf4
• Synonyms: AV314029; BC042423; ENSMUSG00000053512; Gm9907; Mef
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217932 representing NM_019680.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTATTGCTCTGCAGCCCAGTGATCTGGTCTTCGAGTTTGCAAGCAACGGGATGGATGATATCCAC
  CAGCTGGAAGACCCTTCAGTGTTCCCAGCCGTGATCGTGGAACAGGTCCCCTATCCTGAATTAGTGCAT
  CTGTGCTCAGGACTGGATCTGGATGAGGTCCACAATGGCATCATAAGGGACAGGACCTTGTGCATGACA
  CAGGATCAGATTTTAGAAGGCAGTATTTTGCTGACAGATGATGATGTATCCACCTCAAATAACGTGTCA
  TCCACTGAAGTCCTATTCAATGTGGCAACTCCCAGTGATGTCCTGGATGAGAAGCAGATCTTCAGCTCA
  CCGGAAGTGCTTTCAGACTCCAACTCTGTTCAGGCCATCAATCTACCCAACTTCCTGCTCTCTACCCCT
  GAACCTGATGACCTGAAGAAGACAAGTGATGCTGGTGACCAGAAGGAGCATTCTGAGGAGGAGAAGGTC
  TCCAGAGAGGAAAATCTTAGGAAGATGGGGAAGGCACGGAAGAGAAACCGAAAGACCAAGAACAACCGA
  AGTACCTCACCTGTCACTGACCCTAGCATGCCTATACGGAAGAAATCCAAAGATGGCAAAGGCAGCACC
  ATTTATCTGTGGGAGTTCCTCCTGGCTCTTCTGCAAGACAGAAACACCTGCCCCAAGTACATTAAGTGG
  ACGCAGAGAGAGAAGGGCATCTTCAAGTTGGTGGACTCCAAGGCTGTGTCCAAGCTGTGGGGCAAGCAG
  AAAAACAAACCTGACATGAACTACGAGACCATGGGGCGAGCCCTGAGATATTATTATCAAAGAGGAATC
  CTGGCCAAAGTGGAAGGGCAGAGGCTGGTATACCAGTTCAAGGAGATGCCTAAGGACTTGGTGGTGATT
  GATGATGAAGAAGAGAGCCCTGAGACACCAGAAGACTCTTCCCAGGCCTCGACTTCCTCCACACCCTCG
  ACCAGTACCATCCGGAGAGCCAGCTCCAGGGTCGGAACCAGAGCCTCCCCCGAGGACAAGGATATCCCT
  CCCTGGGAGAAGCCAAAAGTCCAACACACTGGTCTCCAGCCATCTGCAAGCCTGGAGTTAGGATTGTCT
  GTGGATGAGGAGGTTCCCACCACCTCCACCATGCTTGCCAGCCCACTACAGAGCCAGGCCAGGCTCACC
  AAAACTGTGAGTTCTTCTCCAGCACCCAGTAACATCCACCTAGGAGTAGCCCCTGTGGGGCCTGGCTCC
  ACTGTCACCCTGCAGACCATCCCGCTGACTACGGTGCTGACCAATGGGCCTCCTGCCAGTACTACTGCT
  CCAACTCAACTCGTTCTTCAGAGTGTCCCGCAGGTGTCAACATTCAAGGACACCTTCACTTTGCAGACC
  TCCTTCCCCCTGAACACCAATTTGCAAGAGAACCAGGTGGCAACCCAAGGGGCCCCACTGATCCTCAGT
  GGTCTCCCCCAACTTCTGGCTGGGGCCAACCCTCAGAGCAACCCAGCTCCATCCCAGGTCATAGGTGCT
  GGGTCAGCAGGGCCTAGCTCTCAGCCCCCTGGGACTGTGATTGCTGCCTTTATCAGGACTTCCAGTGGC
  ACATCAGTGCCTGTGGTAAAGGAGGGGCCCTTGAGGTCCTCTTCATATGTGCAGGGTGTGGTTACTGGG
  GCACCCGTGGAGGGGCTGTTGGTTCCTGAAGAGACCCTGAGAGAGCTCCTGAGAGATCAGGGTCATCTT
  CAGCCACTTCCGAGTCAGGTGCTCTCAAGGGGTTCCCATAACCTGAGCCTTGTGGGGAACCAGACTCTC
  TCTCCCCCTAGCCACCCCACTGTTGGGCTAACCCCAGTGGCTGAGCTCGAGCTCTCGTCAGGCTCAGGG
  CCCCTGTTCGTAACTGAGCCTAGTGTGACCAGATCCCCAACCCAGGCCCCTTTCTCCCCATTCAATCCC
  ACTTCCCTCATTAAGATGGAACCCCAAGATATA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by MR217932
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAIALQPSDLVFEFASNGMDDIHQLEDPSVFPAVIVEQVPYPELVHLCSGLDLDEVHNGIIRDRTLCMT
  QDQILEGSILLTDDDVSTSNNVSSTEVLFNVATPSDVLDEKQIFSSPEVLSDSNSVQAINLPNFLLSTP
  EPDDLKKTSDAGDQKEHSEEEKVSREENLRKMGKARKRNRKTKNNRSTSPVTDPSMPIRKKSKDGKGST
  IYLWEFLLALLQDRNTCPKYIKWTQREKGIFKLVDSKAVSKLWGKQKNKPDMNYETMGRALRYYYQRGI
  LAKVEGQRLVYQFKEMPKDLVVIDDEEESPETPEDSSQASTSSTPSTSTIRRASSRVGTRASPEDKDIP
  PWEKPKVQHTGLQPSASLELGLSVDEEVPTTSTMLASPLQSQARLTKTVSSSPAPSNIHLGVAPVGPGS
  TVTLQTIPLTTVLTNGPPASTTAPTQLVLQSVPQVSTFKDTFTLQTSFPLNTNLQENQVATQGAPLILS
  GLPQLLAGANPQSNPAPSQVIGAGSAGPSSQPPGTVIAAFIRTSSGTSVPVVKEGPLRSSSYVQGVVTG
  APVEGLLVPEETLRELLRDQGHLQPLPSQVLSRGSHNLSLVGNQTLSPPSHPTVGLTPVAELELSSGSG
  PLFVTEPSVTRSPTQAPFSPFNPTSLIKMEPQDI
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019680
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 5847 bp
• RefSeq ORF: 1968 bp
• Locus ID: 56501
• UniProt ID: Q9Z2U4
• Cytogenetics: X A5
• MW: 70.8 kDa
• Gene Summary: Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of LYZ. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2. Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway.[UniProtKB/Swiss-Prot Function]