Cramp1l (NM_020608) Mouse Tagged ORF Clone

CAT#: MR217849

Cramp1l (Myc-DDK-tagged) - Mouse Crm, cramped-like (Drosophila) (Cramp1l)

Product Images

Circular map for MR217849

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cramp1l
• Synonyms: 5830477H08Rik; AI256853; AI790734; Cramp1; mKIAA1426; Tce4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217849 representing NM_020608
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGTGAAGTTGGGCGACGCCGGCAGCGGGGAGGAAGGGCTCAAGAAGCTGGGCAAGCGGACGGCCG
  ATGAGGAGTCGCTGGACGGAGAGGGGCCCGGCGGCGCGGACGCGGCGGACAGCAGCAGCACAAAGAGGGA
  CGGGCAGACCCCTCGGGCCTCCGGAGCCCCCGCGCCACCCAGAGGGCTGCCGACCCCGTCTCCACCGCAG
  GGCAGCCCCCAGGACCAGCACCATTTCCTAAGGTCCAGCGTGCGACCCCAGAGCAAGAGGCCCAGGAAGG
  ACGCACCCTGTGCTTTGGGCAGTGGTGGTGCCAGCGGTTCCGGGCCGCGAGGAAAAGGAAGTGACGGTGG
  TGCATCATCTTCTGGAAACGTGTCTGGGGCTACCCCTGCCACTCCTGCAGGAGGCTCGCGCTCCTCCTCC
  CGGAACATAGGATCTTCAGGGCCTGAGAAAGAAGAAGGAAAGAAGGTCCGAAGGCAGTGGGAGTCGTGGA
  GCACCGAGGACAAGAACACCTTCTTCGAGGGACTGTATGAGCATGGAAAAGACTTTGAAGCCATTCAGAA
  CAACATTGCTCTAAAGTACAAGAAGAAGGGCAAGCCTGCAAGCATGGTGAAGAACAAAGAGCAGGTCCGG
  CATTTTTACTACCGCACCTGGCACAAGATTACCAAATACATTGACTTTGACAATGTGTTCTCTCGAGGGC
  TGAAGAAGTCATCCCAGGAACTCTATGGTCTCATCTGCTATGGCGAGCTACGCAAGAAGATTGGGGGTTG
  TATGGATGACAAGAATGCAACAAAGTTGAATGAACTCATTCAGGTTGGAGCCACCACAGTACGTTATAAA
  GGGAGAAATCTCCGGATCAAGGCACCCATGTGCCGAGCCCTAAAGAAACTCTGTGATCCAGATGGTCTAA
  GTGATGAAGAGGACCAGAAGCCAGTGCGCCTACCCCTGAAAGTCCCAGTAGAATTACAACCAAGGAACAA
  CCACGCTTGGGCACGAGTACAGAGCCTGGCCCAGAACCCACGTCTCAGGATGATTGTGGAGCTACATAGA
  AAAGTCTCTAGCCTCATTGAGTTCCTGAAGCAGAAGTGGGCACTCCATGAAGTACGAATTCGGAAGACAC
  TTGAGGAGAGGCAGCTGCAGGGTTCAAGCACAGTGCAGACCCAGGAGAAGGTGGCATTGCACCTGTTCCC
  AGGCGAGAACTGCACGCTGACACCACTACCCGGTGTAGCCCGTGTGGTACACTCCAAGGCCTTCTGCACT
  GTACACTGGCAAGAGGGTGGCCGGTGCAAACAGGGCACTAAGGACATACACTCACTGCCTCCAGCACAGA
  TCCTCGGCATCCAGAGTGGACAGGGAATAGCCCGGGGCCAGCTGAAGTGCCAGAGGAGCAGTGCTGAAGG
  CAAGGGTGGGGGGCGGCTCCTTCCCACTGCAGATGCTTCACAGAGCTCAGGGGAAAGTTCCCCTGAGAGT
  GCTCCTGCAGAGGGAGCAGCTCCAAGTCTGAGCAGCCCAGATGCCCCCGACAGGCCTCATGGGCTCCAGG
  ACTCTGGACCACACCTTGAGAAGACCCCTGTGACAGCCCTTGCAGTGGGTAGGGACAGCCCTATTCAAGA
  ATCTGGGGCCCTGCCGTGTCCCTGTGGCCAGCCCCCAGACTTGGAAGATGAACTCTCACTCCTTGACCCC
  TTCCCCCGATACCTGAAGTCGTGCCAGGATCTCATCATCCCAGAGCAGTGCCGCCGTGCAGACACACGAT
  CTGGGAGAGAGCATCCTCCTCTCGGCAGCGTAGCTTCCCCAGAGACCCTCACACCCAGCAGCGGGGCGGT
  CGCTGACAGTGCTCCCCCTGGCCTGGACCCTCAACCTGGCACTGATCATCAACCTGACACCAGGCTTCAG
  TCAGATATTTGTACTAAAGAGCTAGCGAACGCATCCTCTGAAGAGTCCCAGGAAAAAGGCAGCCCCTCAG
  AACATCTGTCGTCTCAGGGACAGCCTGCTACCAGGCCTTCCAAGGAAGTCCCTGCCAGCCAGCTGGCCCA
  GCAGCTACGTGAAGAAGGCTGGAGCTTGCAGACCTCTGAAAGCCTCACACTGGCTGAAGTCTACCTCATG
  ATGGGCAAGCCGACCAAACTGCAGCTGGAGTATGATTGGCTGGCTGTGCTGGGCCCCGAGAGCCAGGCAC
  CCACAGCACAGGGCCAGACTGCACTATCCCGCTCCTCGCCCTCAGCCCTCCACCAGCAACGCCTCCTGAG
  CTGCCTTCTGAGGCTCATCTCCACTGAGGTCCACCCCAAGATGGCTTTTGAAGCAAACACAGCATCAACG
  GCTTCAGTCAGGCCCACCCAGGAGGAACAGTCCACAACTCCACCAGGGAAGGTAGTGACCATCAACTCTC
  GGAGTCCCCGCTGTTCTCGAAACCCATCTACCCTCCGAAGCAACAAGACTTTCCCATCTGCTTCTGCCCC
  TTGCTCCCCAGGTTTGAGAAACCCCCCAAGGCCTCTCTTGGTGGCTGGTCCCTCCAGCACAGGAAGCAGT
  GACTCAGATGGTGGCCTCTTTGCTGTCCCAACGACTTTGCCACCAAATAGTCGACATGGAAAGCTCTTCT
  CTCCCAGTAAGGAGGCGGAGCTGACTTTTCGGCAGCACTTGGACTCCATCAGCATACAGTCAGACTTTTT
  CTCACCAAAGCCCAAGAAACTACGGAAACGGCACTTAAGAAAGCCACTGGTGGTCCAGAGGACACTGCTC
  CCTAGACCTTCTGAAAACCAGTCCCACAATGTGTGCTCCTTCTCCATCCTGTCTAACTCTCCTATAGCTG
  GGAGAGGCTGGTTCCGGCCCATCCAGTCTTCTTTGACCAAAGCAGCTCTGTCTCGACCGATAGTGCCCAA
  GGTCCTCCCATCTCAGGCCACCAGTCACCTGCCCAGTGCCATCGACTTAGCAGCTCAAAGTGCTGGCATC
  ATCCCTGGAAGTCCCTTGCCAATTTTAGATACTGATGGCTCATCTGGCATCTCTCCACTGTCTTCAGAAC
  AAGCAACCACTGCCATCTCGGGGCAGGGTGACACTGGACCACACCAGAACAGAGACCCTGTTACTGCTGT
  AAGGGGCACTAATGACCCATTTATCAGTGTTACAAGACCAGAGCAGGAGCCAATGACAGATGGTTTCCAG
  GGATCACCTGCTCTGACCTTACCTGAGCTGTCCAAGGCTAATCTCCAGAATGGCCTGTCCATACCTTTAC
  CCTCATCGGAGAGCTCCAGCACCCGTCTCTCCCCACCCAATGTTTCTGCACTGCTGGACATATCCCTACC
  TGGTCCACCTGAGGATGTTCTCTCACAGGGAGAACCTGCCACACAGATCAGTGACTCCATCATTGAAATT
  GCCATCAGCTCTGGCCAGTACAGTGAAGGAGTTCCCCTTTCTCCAGCAAAACTGAACGGCAGTGACAGTT
  CCAAGAGTCTTCCCTCCCCATCTAGCAGCCCCCAGCCCAACTGGATTGCCTCTCCCACTCATGATCCCCA
  GTGGTATCCCAGTGACTCTGCAGATTCCTCACTCAGCAGCTTGTTCGCCAGCTTCATATCTCCAGAGAAG
  AGCAGAAAGATGCTACCAACTACTGTTGGGGCCAACAGCGGCACATCTTTGCTTGGCCCTAGCTTGTTGG
  ATGGAAATTCAAGGGACTCTTTTGTGTCCAGGTCTCTGGCTGATGTTGCAGAAGTGGTGGATTCCCAGTT
  GGTGTGCATGATGAACGAGAACAGCATTGACTACATATCTCGGTTTAATGACCTGGCCCAAGAATTGTCC
  ATCACTGAGCCTGGCCGCCGGGAGGTTCTTTTTGATGGTGGAGGAGGTGGTAATCCTGTTGGTGACCTCT
  CTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217849 representing NM_020608
  Red=Cloning site Green=Tags(s)
  
  MTVKLGDAGSGEEGLKKLGKRTADEESLDGEGPGGADAADSSSTKRDGQTPRASGAPAPPRGLPTPSPPQ
  GSPQDQHHFLRSSVRPQSKRPRKDAPCALGSGGASGSGPRGKGSDGGASSSGNVSGATPATPAGGSRSSS
  RNIGSSGPEKEEGKKVRRQWESWSTEDKNTFFEGLYEHGKDFEAIQNNIALKYKKKGKPASMVKNKEQVR
  HFYYRTWHKITKYIDFDNVFSRGLKKSSQELYGLICYGELRKKIGGCMDDKNATKLNELIQVGATTVRYK
  GRNLRIKAPMCRALKKLCDPDGLSDEEDQKPVRLPLKVPVELQPRNNHAWARVQSLAQNPRLRMIVELHR
  KVSSLIEFLKQKWALHEVRIRKTLEERQLQGSSTVQTQEKVALHLFPGENCTLTPLPGVARVVHSKAFCT
  VHWQEGGRCKQGTKDIHSLPPAQILGIQSGQGIARGQLKCQRSSAEGKGGGRLLPTADASQSSGESSPES
  APAEGAAPSLSSPDAPDRPHGLQDSGPHLEKTPVTALAVGRDSPIQESGALPCPCGQPPDLEDELSLLDP
  FPRYLKSCQDLIIPEQCRRADTRSGREHPPLGSVASPETLTPSSGAVADSAPPGLDPQPGTDHQPDTRLQ
  SDICTKELANASSEESQEKGSPSEHLSSQGQPATRPSKEVPASQLAQQLREEGWSLQTSESLTLAEVYLM
  MGKPTKLQLEYDWLAVLGPESQAPTAQGQTALSRSSPSALHQQRLLSCLLRLISTEVHPKMAFEANTAST
  ASVRPTQEEQSTTPPGKVVTINSRSPRCSRNPSTLRSNKTFPSASAPCSPGLRNPPRPLLVAGPSSTGSS
  DSDGGLFAVPTTLPPNSRHGKLFSPSKEAELTFRQHLDSISIQSDFFSPKPKKLRKRHLRKPLVVQRTLL
  PRPSENQSHNVCSFSILSNSPIAGRGWFRPIQSSLTKAALSRPIVPKVLPSQATSHLPSAIDLAAQSAGI
  IPGSPLPILDTDGSSGISPLSSEQATTAISGQGDTGPHQNRDPVTAVRGTNDPFISVTRPEQEPMTDGFQ
  GSPALTLPELSKANLQNGLSIPLPSSESSSTRLSPPNVSALLDISLPGPPEDVLSQGEPATQISDSIIEI
  AISSGQYSEGVPLSPAKLNGSDSSKSLPSPSSSPQPNWIASPTHDPQWYPSDSADSSLSSLFASFISPEK
  SRKMLPTTVGANSGTSLLGPSLLDGNSRDSFVSRSLADVAEVVDSQLVCMMNENSIDYISRFNDLAQELS
  ITEPGRREVLFDGGGGGNPVGDLSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020608
• ORF Size: 3855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020608.3, NP_065633.2
• RefSeq Size: 7534 bp
• RefSeq ORF: 3858 bp
• Locus ID: 57354
• UniProt ID: Q6PG95
• Cytogenetics: 17 A3.3
• MW: 137.1 kDa