Arfgef3 (NM_001033258) Mouse Tagged ORF Clone
CAT#: MR217542
- TrueORF®
D10Bwg1379e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, Brigham & Women's Genetics 1379 expressed (D10Bwg1379e)
ORF Plasmid: tGFP
"NM_001033258" in other vectors (2)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Arfgef3 |
Synonyms | 9630004D11; B930094H20Rik; BIG3; D10Bwg1379e; mKIAA1244 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217542 representing NM_001033258
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGATCCTGAGGAAGCTGCAGAGAGATGCGTCCGGGAGCAAATACAAAGCCATCAAAGAGAGCT GCACCTGGGCCCTGGAAACCCTAGGTGGCCTGGACACTGTTGTGAAGATCCCTCCCCACTTGTTGAGGGA GAAATGCTTGCTGCCTCTCCAGTTGGCTTTGGAATCAAAAAATGTGAAGCTGGCCCAACATGCTTTGGCA GGAATGCAGAAGCTCCTGTCAGAAGAGAGATTTGTCTCCATGGAGACAGACTCCGATGAGAAGCAGCTGC TCAATCAGATCCTGAATGCAGTGAAAGTGACACCGTCACTCAATGAGGACCTGCAGGTGGAAGTGATGAA GGTTTTACTGTGCATCACCTACACCCCGACATTTGACATGAATGGGAGTGCCGTTCTGAAGATCGCAGAG GTGTGCATCGAGACCTACACGTGCAGCTGCCACCAGCGCAGTATCAACACTGCCGTGCGTGCAACGCTCA GTCAGATGCTGGGTGACCTGACTTTACAGTTACGGCAGAGACAGGAGAATACGATAATTGAAAACCCAGA TGCCCCACAGGAATTCAGGAGTCAAGGCTTAACAGTAGAGGCCCTTTGTGATGACGTCATCTCTGTGCTC GCAGTTCTGTGTGAGAAGCTCCAAGCCTCCATAAATGACAGCCAGCAGCTACAGCTTCTGTACCTGGAAT GCATCCTCTCCGTGCTCAGCAGCTCCTCTTCCTCCATGCACCTGCACAGGGGCTTCACAGACCTCATCTG GAAAAGCCTCTGCCCTGCTCTCGTGGTGATCCTGGGGAACCCTATTCACGACAAAACAATCACCTCTGCT CACAGCACCAGCACCAGCACCAGCATGGAGTCGGACTCTGCCTCTCTTGGTGTGTCTGACCACGGCCGGG GCTCAGGCTGCTCCTGCACTGCACCCACCCTCAGTGGGCCTGTGGCCCGCACCATCTATTACCTTGCAGC TGAGCTTGTCCGCTTAGTGGGGTCAGTGGACTCCATGAAGCCAGTGCTCCAGTCCCTCTATCACCGAGTG CTCCTGTATCCCCCACCCCAGCACCGAGTGGAAGCCATCAAGATCATGAAAGAGATACTTGGGAGCCCAC AGCGTCTGTATGACTTGGCAGGACCAAGCTCTATTGAGTCTGAACCAAGGAAAAGATCTATTTCCAAAAG AAAGTCTCACTTGGATCTTCTCAAACTCATCATGGACGGCATGACAGAAGCATGCATCAAAGGAGGCATT GAAGCCTGCTACGCTGCTGTGTCCTGTGTCTGCACCTTGCTTGGGGCTCTGGATGAACTCAGCCAAGGGA AAGGCTTGAATGACACTCAAGTGCAGCAGCTGCTTCTGCGCCTTGAAGAGCTGAGGGATGGGGCCGAGTC GAGCCGGGACTCCATGGAAATTAATGAGGCTGACTTCCGCTGGCAGCGGAGAGTGCTGTCTTCGGAGCAC ACTCCATGGGAGTCAGGCAATGAGAGGAGCCCTGACATCAGCATCAGTGTCACCACAGACACAGGCCAGA CTACCTTGGAAGGAGAATTGGGTCAGACGACACCAGAGGACCACAAGAATGGGCTCAAGTCACCAGCCAT TCAGGAAGGCAAGGGGACCATGGGCAAAGTGTCAGAACCTGAGGCTATAGACCAGCCAGATGTGGTTCAG AGAAGCCACACAGTCCCTTACCCCGATATCACCAACTTCCTGTCAGTGGACTGCAGGACAAGGTCCTACG GATCTAGGTATAGTGAGAGCAACTTCAGTGTAGATGACCAAGACCTGTCGAGGACTGAATTTGATTCCTG TGATCAGTATTCCATGGCGGCAGAAAAGGACTCAGGGAGGTCTGATGTGTCAGACATTGGGTCAGACAAC TGTTCCCTGGCTGATGAAGAACAGACCCCTCGGGACTACATAGGCCATCGGTCCCTTCGAACCGCTGCTT TGTCTCTCAAACTGCTAAAGAACCAGGAGGCAGACCAGCACAGCGCCCGACTGTTTATACAGTCCCTTGA GGGCCTCCTACCCCGGCTGCTGGCCCTGTCCAGTGTGGAAGAGGTGGACTCCGCCCTTCAGAACTTTGCC TCTACTTTCTGCTCAGGCATGATGCACTCTCCTGGCTTTGATGGAGGCAGTAGCCTGAGCTTCCAGATGC TGATGAATGCAGACAGCTTGTACACAGCGGCACACTGTGCCCTACTGCTCAACCTGAAGCTCTCCCACGG TGACTACTACCGGAAGCGACCAACCGTGGCACCAGGCATGATGAAAGAGTTCATGAAACAGGTGCAGACC AGCGGCGTGCTCATGGTCTTCTCCCAGGCCTGGCTCGAGGAACTGTACCATCAGGTGCTGGACAGAAACA TGCTGGGAGAAGCTGGCTACTGGGGCAGCCCTGAGGACAACAGCCTTCCCCTCATCACCATGCTCACCGA TATCGATGGTCTAGAGAGTAGCGCAATCGGTGGCCAGTTGATGGCGTCAGCTTCAGTGGAGTCACCTTTC ACCCAGAGCAGGAGACTTGATGACTCCACGGTAGCAGGTGTTGCATTTGCCCGTTATATTTTGGTTGGCT GCTGGAAGAACCTGATTGATACTCTGTCAACCCCCTTGACTGGTCGGATGGCAGGAAGCTCCAAGGGGTT GGCCTTCATCCTTGGAGCTGAGGGTATCAAAGAGCAGAACCAGAAAGAGCGAGATGCCATCTGCATGAGC CTTGATGGGCTAAGGAAAGCTGCGCGACTGAGCTGTGCACTCGGGGTCGCTGCTAACTGTGCCTCAGCTC TTGCCCAAATGGCAGCTGCATCCTGTGTCCAAGAAGAGAAAGAAGAAAGGCAATCCCAAGAGCCCAGTGA TGCCCTAGCACAAGTGAAACTAAAAGTGGAGCAGAAACTGGAGCAGATGGGGAAGGTGCAGGGGGTGTGG CTGCACACAGCCCACGTCTTGTGCATGGATGCCATTCTCAGCGTAGGCCTGGAGATGGGAAGCCACAACC CGGACTGCTGGCCACACGTGTTCAGGGTGTGTGAGTATGTGGGTACACTGGAGCACACCCACTTCAGCGA TGGTATCTCCCAGCCCCCTCTGACCATCCATCAGCCACAGAAGACATCTGGGAGCTCTGGCCTCCTTGGG GAAATTGAGTTTAAGAGCTCATCCCAGGAGCAGAGCCTGGAGCAGGGGCCTTCCCTGAACACAGCCCCTG TGGTCCAGCCACACTCCATCCAGGAGCTTGTTCGGGAATGTAGCCGTGGCCGGACCTCGGACTTCCGGGG AGGTAGTCTGAGTGGGAACAGTGCTGCCAAGGTGGTGCTCAGCCTTTCCACCCAGGCCGACAGGCTCTTT GATGACGCGACCGACAAGTTGAACTTAACAGCCTTGGGAGGGTTCCTCTACCAACTCAAAAAAGCATCTC AGTCCCAGCTTTTCCATTCGGTGACAGACACAGTCGATTACTCTCTGACAATGCCAGGTGAAGTCAAGTC TACCCAGGATCAAAAAAGCGCTCTCCACCTGTTCCGCCTGGGGGATGCCATGCTGAGGATTGTGAGGAGC AAAGCCCGGCCCCTGCTCCACGTGATGCGCTGCTGGAGCCTCGTGGCCCCGCACCTGGTGGAGGCTGCCT GCCATAAAGAACGCCATGTGTCTCAGAAGGCCGTTTCCTTCATCCATGATATCTTGACAGAGGTTCTCAC TGACTGGAGTGAGCCACCTCACTTCCACTTTAACGAAGCACTCTTCCGGCCCTTTGAGCGCATCATGCAG CTAGAGCTCTGTGATGAAGATGTCCAAGACCAGGTGGTCACCTCCATTGGTGAGCTGGTTGAGGTGTGCT CTGCACAGATCCAGTCGGGATGGCGACCCCTGTTCAGTGCTCTGGAGACCGTGCGCAGTGGGAACAAGTC AGAGGTGAAGGAGTACCTGGTTGGGGACTACTCTATGGGAAAAGGCCAGGCTCCTGTGTTCGATGTGTTT GAAGCTTTTCTCAATACCGACAACATCCAGGTCTTTGCTAACGCAGCTACTAGTTACATCATGTGCCTTA TGAAATTTGTCAAGGGGCTGGGGGAGGTAGACTGTAAAGAGATTGGAGACTGTGTTCCTGGAGCTGGAGC TACATCTACAGACCTGTGCCTGCCTGCCTTGGATTACCTCAGACGTTGTTCTCAGTTATTGGCCAAGATC TACAAAATGCCCTTAAAGCCAATATTCCTTAGTGGGCGACTTGCCAGTTTGCCACGAAGGCTTCAGGAGC AGTCGGCAAGCAGTGAAGATGGAATTGAATCAGTCCTGTCAGATTTTGATGATGACACTGGTCTGATAGA AGTCTGGATCATCCTGTTGGAGCAGCTAACAGCAGCTGTGTCCAACTGTCCAAGGCAACACCAACCACCC ACCTTAGATTTACTCTTTGAGCTGTTGAGAGATGTTACCAAAACACCTGGCCCTGGGTTTGGAATCTATG CAGTTGTTCACCTTCTCCTTCCTGTGATGTCTCTGTGGCTCCTCCGTAGCCACAAGGACCATTCCTACTG GGATGTGGCATCTGCTAACTTCAAGCACGCCATTGGTCTGTCCTGCGAGCTGGTGGTGGAACACATTCAA AGCTTTCTACACTCAGACATCAGGTATGAGAGTATGATCAACACCATGCTGAAGGATCTCTTTGAGCTAC TGGTAGTATGCGTGGCCAAGCCCACAGAAACCATCTCCAGGGTGGGCTGTTCCTGCATTAGGTACGTCCT CGTGACAGCAGGTCCCGTGTTCACGGAGGAGATGTGGAGACTTGCATGCTGTGCCCTTCAGGACGCCTTC TCAGCCACCCTCAAGCCAGTGAAGGACCTGCTAGGCTGCTTCCACGGTGGTACAGAGGGCTTCAGTGGGG AAGGCTGCCAGGTGCGCGTAGCAGCACCCTCTTCCTCCCCGAGTGCTGAGGCTGAGTACTGGCGCATCCG GGCCATGGCCCAGCAGGTGTTTATGTTGGACACCCAGTGCTCACCAAAGACTCCAAACAACTTCGATCAC GCTCAGTCCTGTCAGCTCATCATTGAACTGCCTCATGATGAGAAACCAAATGGACATGCCAAGAAAAGTG TCTCTTTCAGGGAGATTGTGGTTAGCTTGCTCTCGCACCAAGTTCTGCTCCAGAACCTGTATGACATTCT GCTGGAAGAGTTTGTCAAAGGCCCCTCTCCGGGAGAAGAGAAGACAGTCCAAGTACCAGACACCAAGCTG GCCGGCTTCCTCAGATACATTTCCATGCAGAACCTGGCTGTGATATTTGACCTGCTGCTGGACTCCTATA GGACTGCCCGGGAATTCGACACCAGCCCAGGGCTGAAATGCCTGCTGAAGAAAGTGTCTGGCATTGGAGG AGCAGCCAACCTCTACCGCCAGTCGGCCATGAGCTTTAACATTTACTTTCACGCCCTGGTTTGCGCGGTT CTCACCAACCAAGAAACTATCACAGCCGAGCAAGTAAAGAAGGTGCTATTCGAAGAGGAGGAGAGGAGCT CGGATTCCTCCCAACAGTGCTCGTCGGAGGATGAAGACATCTTTGAGGAGACTGCGCAGGTGAGCCCCCC ACGAGGCAAGGAGAAAAGGCAATGGCGGGCACGCCTGCCGTCGCTCAGTGTGCAGCCAGTAAGCAATGCA GACTGGGTGTGGCTGGTCAAGAGGCTGCACAAGCTGTGCATGGAGCTCTGTAACCACTACATCCAGATGC ACCTGGACCTGGAGAGCAGCTTGGAGGAGCCACTTACCTTCAAGAGCGACCCGTTCTTCATTCTCCCCTC CTTCCAGTCCGAATCGTCCACCCCATCAACAGGTGGCTTCTCCGGGAAAAACACCCCCTCGGAAGATGAC CGCAGGGAGCACCTGAGTGAGCCTCAGAGTCTGAGGGTCGGCAGTGGGGACATGCTGATGCTGCCTCCCA GCCCCAAGACAGAGAAGAAGGATCCTGGCCGCAAGAAAGAGTGGTGGGAGAGTGCAGGGAACAAGATTTG CACAATGGCCGCTGACAAGACCATCTCAAAGCTGATGACTGAGTACAAGAAGAGAAGGCAGCCACATAAC CTGCCCCCCTTCCCCAAGGAAGTCAAAGTAGATAAGAAAGGGGAGCCCTTGGGGCCCCGGGGTCCTGACT CCCCACTGCTTCAGCGGCCACAGCATCTCATAGACCAAGGGCAGATGCGCCATTCCTTCAGCGCAGGCCC AGAGCTGCTGAGGCAGGAGAAGAGACCTCGGTCAGGCTCCACAGGAAGCTCCCTTAGTGTCTCTGTACGG GATGCAGAAGCGCAGATCCAGGCGTGGACCAACATGGTGCTAACTGTTCTCAATCAGATTCAGATCCTTC CAGACCAGACCTTCACTGCTCTCCAGCCAGCTGTGTTCCCATGCATCAGCCAGCTGACCTGTCACGTGAC TGACATCCGAGTCCGTCAAGCTGTGAGGGAATGGCTGGGAAGAGTGGGCCGTGTCTATGACATCATCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217542 representing NM_001033258
Red=Cloning site Green=Tags(s) MEEILRKLQRDASGSKYKAIKESCTWALETLGGLDTVVKIPPHLLREKCLLPLQLALESKNVKLAQHALA GMQKLLSEERFVSMETDSDEKQLLNQILNAVKVTPSLNEDLQVEVMKVLLCITYTPTFDMNGSAVLKIAE VCIETYTCSCHQRSINTAVRATLSQMLGDLTLQLRQRQENTIIENPDAPQEFRSQGLTVEALCDDVISVL AVLCEKLQASINDSQQLQLLYLECILSVLSSSSSSMHLHRGFTDLIWKSLCPALVVILGNPIHDKTITSA HSTSTSTSMESDSASLGVSDHGRGSGCSCTAPTLSGPVARTIYYLAAELVRLVGSVDSMKPVLQSLYHRV LLYPPPQHRVEAIKIMKEILGSPQRLYDLAGPSSIESEPRKRSISKRKSHLDLLKLIMDGMTEACIKGGI EACYAAVSCVCTLLGALDELSQGKGLNDTQVQQLLLRLEELRDGAESSRDSMEINEADFRWQRRVLSSEH TPWESGNERSPDISISVTTDTGQTTLEGELGQTTPEDHKNGLKSPAIQEGKGTMGKVSEPEAIDQPDVVQ RSHTVPYPDITNFLSVDCRTRSYGSRYSESNFSVDDQDLSRTEFDSCDQYSMAAEKDSGRSDVSDIGSDN CSLADEEQTPRDYIGHRSLRTAALSLKLLKNQEADQHSARLFIQSLEGLLPRLLALSSVEEVDSALQNFA STFCSGMMHSPGFDGGSSLSFQMLMNADSLYTAAHCALLLNLKLSHGDYYRKRPTVAPGMMKEFMKQVQT SGVLMVFSQAWLEELYHQVLDRNMLGEAGYWGSPEDNSLPLITMLTDIDGLESSAIGGQLMASASVESPF TQSRRLDDSTVAGVAFARYILVGCWKNLIDTLSTPLTGRMAGSSKGLAFILGAEGIKEQNQKERDAICMS LDGLRKAARLSCALGVAANCASALAQMAAASCVQEEKEERQSQEPSDALAQVKLKVEQKLEQMGKVQGVW LHTAHVLCMDAILSVGLEMGSHNPDCWPHVFRVCEYVGTLEHTHFSDGISQPPLTIHQPQKTSGSSGLLG EIEFKSSSQEQSLEQGPSLNTAPVVQPHSIQELVRECSRGRTSDFRGGSLSGNSAAKVVLSLSTQADRLF DDATDKLNLTALGGFLYQLKKASQSQLFHSVTDTVDYSLTMPGEVKSTQDQKSALHLFRLGDAMLRIVRS KARPLLHVMRCWSLVAPHLVEAACHKERHVSQKAVSFIHDILTEVLTDWSEPPHFHFNEALFRPFERIMQ LELCDEDVQDQVVTSIGELVEVCSAQIQSGWRPLFSALETVRSGNKSEVKEYLVGDYSMGKGQAPVFDVF EAFLNTDNIQVFANAATSYIMCLMKFVKGLGEVDCKEIGDCVPGAGATSTDLCLPALDYLRRCSQLLAKI YKMPLKPIFLSGRLASLPRRLQEQSASSEDGIESVLSDFDDDTGLIEVWIILLEQLTAAVSNCPRQHQPP TLDLLFELLRDVTKTPGPGFGIYAVVHLLLPVMSLWLLRSHKDHSYWDVASANFKHAIGLSCELVVEHIQ SFLHSDIRYESMINTMLKDLFELLVVCVAKPTETISRVGCSCIRYVLVTAGPVFTEEMWRLACCALQDAF SATLKPVKDLLGCFHGGTEGFSGEGCQVRVAAPSSSPSAEAEYWRIRAMAQQVFMLDTQCSPKTPNNFDH AQSCQLIIELPHDEKPNGHAKKSVSFREIVVSLLSHQVLLQNLYDILLEEFVKGPSPGEEKTVQVPDTKL AGFLRYISMQNLAVIFDLLLDSYRTAREFDTSPGLKCLLKKVSGIGGAANLYRQSAMSFNIYFHALVCAV LTNQETITAEQVKKVLFEEEERSSDSSQQCSSEDEDIFEETAQVSPPRGKEKRQWRARLPSLSVQPVSNA DWVWLVKRLHKLCMELCNHYIQMHLDLESSLEEPLTFKSDPFFILPSFQSESSTPSTGGFSGKNTPSEDD RREHLSEPQSLRVGSGDMLMLPPSPKTEKKDPGRKKEWWESAGNKICTMAADKTISKLMTEYKKRRQPHN LPPFPKEVKVDKKGEPLGPRGPDSPLLQRPQHLIDQGQMRHSFSAGPELLRQEKRPRSGSTGSSLSVSVR DAEAQIQAWTNMVLTVLNQIQILPDQTFTALQPAVFPCISQLTCHVTDIRVRQAVREWLGRVGRVYDIIT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033258 |
ORF Size | 6510 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033258.4, NP_001028430.1 |
RefSeq Size | 8216 bp |
RefSeq ORF | 6513 bp |
Locus ID | 215821 |
UniProt ID | Q3UGY8 |
Cytogenetics | 10 7.79 cM |
MW | 240.1 kDa |
Gene Summary | Participates in the regulation of systemic glucose homeostasis, where it negatively regulates insulin granule biogenesis in pancreatic islet beta cells (PubMed:24711543). Also regulates glucagon granule production in pancreatic alpha cells (PubMed:25737957). Inhibits nuclear translocation of the transcriptional coregulator PHB2 and may enhance estrogen receptor alpha (ESR1) transcriptional activity in breast cancer cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC225208 | D10Bwg1379e (untagged) - Mouse DNA segment, Chr 10, Brigham & Women's Genetics 1379 expressed (D10Bwg1379e), (10ug) |
USD 1,971.00 |
|
MG217542 | D10Bwg1379e (tGFP-tagged) - Mouse DNA segment Chr 10 Brigham & Women's Genetics 1379 expressed (D10Bwg1379e), (10ug) |
USD 1,991.00 |
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