Map7 (NM_008635) Mouse Tagged ORF Clone
CAT#: MR217450
- TrueORF®
Map7 (Myc-DDK-tagged) - Mouse microtubule-associated protein 7 (Mtap7), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_008635" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Map7 |
Synonyms | E-MAP-115; MAP-7; mshi; mste; Mtap7; R75000; ste |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217450 representing NM_008635
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCAGGGAGCTGGCGGCGACGGCCACAGGGGCGGCGACGGCGCTACGCACAGCGACCCAGCGT CTGATGGCTATAAAGTACAAGAGAAGAGAACTGCCCCCAGCCGCCCCACTTCTACAGTTTCAGGACAAAC TAGCAACCACTCAGGAAACAAACCAGATCCCCCACCTGTGCTACGGGTTGATGACCGGCAGCGGCTAGCC AGAGAACGCCGAGAGGAACGAGAAAAACAGCTAGCTGCACGAGAAACCGTCTGGCTAGAGAGAGAAGAAC GAGCCCGGCAGCACTATGAGAGACATCTGGAGGCGAGGAAGAAGAAGCTGGAGGACCAACGGCTGAAGGA GGAGCGGAGGAGGGCTGCGGTGGAGGAGAAGCGGAGGCAGAGGCTGGAGGAGGACAAGGAGCGCCATGAA GCTGTTGTCCGACGGACGATGGAAAGGAGCCAGAAGCCAAGGCAGAAGTCTAACCGCTGGTCCTGGGGAA GCCCTCTCCATGGGAGCTCGAGCATCCACAGTGGAGATCCAGACAGGCGCTCAGTTTCCACCATGAATCT TTCGAAACATGTTGATCCTGTCATTAGCAAGCGGCTCTCCTCCTCGTCTGCAACTTTGCTAAACTCTCCA GATAGAGCTCGCCGCCTGCAGCTCAGCCCCTGGGAGAGCAGCGTTGTTAGCAGACTTCTGACGCCCACAC ATTCGTTCCTGGCCAGAAGCAAAAGCACGGCCGCCTTGTCTGGAGACACAGCATCTTGCAGCCCCATCAT CATGCCCTTCAAAGCTGCACACTCTAGAAACCCAGTGGACCGACCAAAACTCTTTGTAACACCGCCTGAG GGCTCTGCACGAAGGAGGACCATTCATGGACTAGCGAGCCATAAAAGAGAGCGAGAAAGAGAACACGTTC CCTTCCACGTTTCCCCGGGCGCCCGCAGGACTCTGTCTCCATCTAATCTCAAAGCGAGGTCACCGGCTCC AGCCCGCCTTTGGCTCCCATCCAAGTCCATGCCTCATCTGCCTGGTACTCCCCGGCCTGCATCCTCCTTG CCTCCCGGCTCAGTCAGAGCTGCTTCCGCTCAGGCCCCCTCCTCCTCTCCTGGCAACATCCGGCCTGTCA AGAGAGAAGTGAAAGTGGAGCCTGAGAAGAAAGACCCTTTACCCGCAGTAAAGAGCAGGGTGCCATTAGT GAAGGTAGAGGAGGTCACAGTCGAAGAGGGGACACCCGTGAAGCCACCTGAGCCTGCTGCTCCAGCCTCG GCCCCCATTGCAACCCCAGCCCCTGCTCCAGCTACGGACCCGGCCCCAGTCCCTGCACCATCATCCACTG TGACTGTCGGTGTAGTTCCTAAGACTTCTGCAGGCACCACCGACCCAGAGGAGGCTACGAGGTTGCTGGC TGAGAAGAGGCGTCTAGCCAGAGAGCAGAGGGAGAAGGAGGAACGGGAGAGGAAGGAAAAGGAAGAGCTG GAGAGACAAAAGATAGAGGAATTGGCCCGTAGGGTGGCTGAAGAGCGAAGTCGCAGGGAAGAAGAAGCCC GCAGGCTGGAAGAGGAGCAGGCTCGAGAGAAAGAGGAGCTGGCGCTGCGTCTGGCTGAGGAGGAGCGGGA GCGGTGGGAGAGGGAGGAGGTGGAGCGCGTGCAGAAGCAGAAAGAAGAAGAAGCCCGAGCCCGGGAGGAG GCAGAGAGGGCTCGGCAGGAACGAGAGAAGCATTTCCAGAAAGAAGAGCAGGAACGGCTGGAGAGGAAGA AGCGACTTGAAGAGATTATGAGAAGAACCAGGAGGACAGAGACCGCTGATAAGAAAACCACTGAGCAAAG AAATGGTGACATAGCCAAAGGAGTTCTCACTGGAGAGCCAGAAGTACCTGCACTGCCGTGTATGGCCTCT TCAGGAAACGGAGAGTCTGCAGAGAGCCCACATGGAGTCGCTTTACAGCAATCAGAAGTGACCACAGAGA GTTCTCCAGATTTGGAAAAACAGCCAAATGAAAACGGAATGTCCATACAAAATGAAAATTTTGAAGAAGT TATAAACTTACCTGTTGGATCAAAAGCGTCCAGATTAGATGTCACCAATGAGAACCCAGAAATTCCTTTG AAACCAATTTTGGCCTTTAATGATGAAGGGACACTTGGGCCCCTACCTCAGGTGGATGGTGTGCAGACAC AACAGACCGCAGAAGTTATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217450 representing NM_008635
Red=Cloning site Green=Tags(s) MAEQGAGGDGHRGGDGATHSDPASDGYKVQEKRTAPSRPTSTVSGQTSNHSGNKPDPPPVLRVDDRQRLA RERREEREKQLAARETVWLEREERARQHYERHLEARKKKLEDQRLKEERRRAAVEEKRRQRLEEDKERHE AVVRRTMERSQKPRQKSNRWSWGSPLHGSSSIHSGDPDRRSVSTMNLSKHVDPVISKRLSSSSATLLNSP DRARRLQLSPWESSVVSRLLTPTHSFLARSKSTAALSGDTASCSPIIMPFKAAHSRNPVDRPKLFVTPPE GSARRRTIHGLASHKREREREHVPFHVSPGARRTLSPSNLKARSPAPARLWLPSKSMPHLPGTPRPASSL PPGSVRAASAQAPSSSPGNIRPVKREVKVEPEKKDPLPAVKSRVPLVKVEEVTVEEGTPVKPPEPAAPAS APIATPAPAPATDPAPVPAPSSTVTVGVVPKTSAGTTDPEEATRLLAEKRRLAREQREKEERERKEKEEL ERQKIEELARRVAEERSRREEEARRLEEEQAREKEELALRLAEEERERWEREEVERVQKQKEEEARAREE AERARQEREKHFQKEEQERLERKKRLEEIMRRTRRTETADKKTTEQRNGDIAKGVLTGEPEVPALPCMAS SGNGESAESPHGVALQQSEVTTESSPDLEKQPNENGMSIQNENFEEVINLPVGSKASRLDVTNENPEIPL KPILAFNDEGTLGPLPQVDGVQTQQTAEVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_008635 |
ORF Size | 2190 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008635.2, NP_032661.2 |
RefSeq Size | 3891 bp |
RefSeq ORF | 2193 bp |
Locus ID | 17761 |
UniProt ID | O88735 |
Cytogenetics | 10 9.75 cM |
MW | 82.4 kDa |
Gene Summary | Microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. Associates with microtubules in a dynamic manner. May play a role in the formation of intercellular contacts. Colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221159 | Map7 (untagged) - Mouse microtubule-associated protein 7 (Mtap7), transcript variant 1, (10ug) |
USD 736.00 |
|
MG217450 | Map7 (tGFP-tagged) - Mouse microtubule-associated protein 7 (Mtap7), (10ug) |
USD 935.00 |
|
MR217450L3 | Lenti ORF clone of Map7 (Myc-DDK-tagged) - Mouse microtubule-associated protein 7 (Mtap7), transcript variant 1 |
USD 1,035.00 |
|
MR217450L4 | Lenti ORF clone of Map7 (mGFP-tagged) - Mouse microtubule-associated protein 7 (Mtap7), transcript variant 1 |
USD 1,035.00 |
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