Rbm14 (NM_019869) Mouse Tagged ORF Clone

CAT#: MR217412

Rbm14 (Myc-DDK-tagged) - Mouse RNA binding motif protein 14 (Rbm14)

Product Images

Circular map for MR217412

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rbm14
• Synonyms: 1300007E16Rik; p16; p16K; PSP2; Sytip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217412 representing NM_019869
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTTTTGTGGGCAATGTCGATGGGGCGGATACGACACCGGAGGAGTTGGCAGCTCTCTTCGCGC
  CCTATGGCACGGTCATGAGCTGCGCCGTCATGAAACAGTTTGCCTTCGTGCACATGCGCGAGAACGCTGG
  CGCGGTGCGCGCCATCGAGGCCCTGCATGGCCACGAGCTGCGTCCAGGTCGCGCGCTCGTGGTGGAGATG
  TCGCGCCCGAGGCCCCTGAACACTTGGAAGATTTTCGTGGGCAATGTATCGGCTGCATGTACAAGTCAGG
  AATTGCGCAGCCTCTTCGAGCGCCGTGGACGCGTCATCGAGTGTGACGTGGTAAAAGACTACGCGTTTGT
  TCACATGGAGAAGGAAGCAGATGCCAAAGCCGCCATCGCGCAGCTCAACGGCAAAGAAGTGAAGGGCAAG
  CGCATCAACGTGGAACTCTCAACCAAGGGGCAGAAGAAGGGGCCTGCCCTGGCTATCCAGTCTGGGGACA
  AGACCAAGAAACCAGGGGCTGGGGACACAGCATTCCCTGGAACTGGTGGCTTCTCTGCCACCTTCGACTA
  CCAGCAGGCTTTTGGCAACAGCACTGGTGGCTTTGATGGGCAAGCCCGTCAGCCCACACCACCATTCTTT
  GGTCGCGACCGCAGCCCCCTGCGCCGTTCACCTCCTCGAGCCTCTTATGTGGCGCCTCTGACGGCCCAGC
  CAGCCACCTACCGGGCCCAACCCTCAGTGTCTCTGGGAGCTGCATATAGGGCCCAGCCTTCCGCCTCTTT
  GGGTGTTGGCTATCGGACTCAGCCCATGGCAGCTCAGGCAGCTTCTTACCGTGCTCAGCCCTCTGTCTCC
  CTTGGGGCCCCATACAGGGGTCAGCTGGCTAGCCCTAGTTCCCAGTCTGCTGCAGCTTCCTCGCTTGGCC
  CATATGGTGGAGTCCAGCCCTCAGCCTCAGCCCTTTCCACTTATGGGGGTCAGGCAGCTGCAGCTTCTTC
  GCTTAATTCCTATGGGGCTCAGGGCTCCTCCCTTGCTTCCTATGGTAACCAGCCATCCTCTTATGGCGCC
  CAGGCTGCCTCTTCGTATGGGGTTCGTGCGGCTGCTTCTTCCTATAACACACAAGGAGCAGCTTCTTCCC
  TAGGTTCCTATGGGGCTCAAGCAGCCTCCTATGGTGCCCAGTCTGCAGCCTCCTCACTAGCTTATGGAGC
  CCAGGCAGCGTCTTACAGTGCCCAGCCCTCAGCCTCTTACAGTGCCCAGTCTGCCCCATATGCTGCACAA
  CAGGCTGCTTCTTATTCTTCCCAGCCTGCTGCTTATGTGGCACAACCAGCCACAGCTGCTGCCTATGCTA
  GCCAGCCAGCTGCCTATGCTGCACAAGCCACTACCCCAATGGCTGGTTCCTATGGGGCTCAACCAGTTGT
  TCAGACCCAGCTGAATAGCTATGGGGCTCAAGCATCTATTGGCCTGTCAGGCTCATATGGAGCTCAGTCT
  GCTGCTGCGGCCACTGGCTCCTATGGGGCGGCAGCTGCTTACGGGGCTCAGCCTTCTGCCACCCTGGCAG
  CTCCTTACCGCACTCAGTCATCAGCCTCATTGGCTGCTTCCTATGCTGCCCAGCAGCATCCCCAGGCTGC
  TGCCTCCTACCGTGGCCAGCCGGGCAGTGCCTACGATGGGACAGGCCAGCCATCAGCAGCCTACCTGTCT
  ATGTCCCAGGGGGCCGTTGCCAACGCCAACAGCACCCCGCCGCCCTATGAGCGTACCCGCCTCTCCCCAC
  CCCGGGCCAGCTACGACGATCCCTACAAAAAGGCTGTCGCCATGTCAAAAAGGTATGGTTCCGACCGGCG
  TTTAGCCGAGCTCTCTGATTACCGCCGTTTATCAGAGTCGCAGCTTTCGTTCCGCCGCTCGCCGACAAAG
  TCCTCGCTGGATTACCGTCGCCTGCCCGATGCCCATTCCGATTACGCACGCTATTCGGGCTCCTATAATG
  ATTACCTGCGGGCAGCTCAGATGCACTCTGGCTACCAGCGCCGCATG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217412 representing NM_019869
  Red=Cloning site Green=Tags(s)
  
  MKIFVGNVDGADTTPEELAALFAPYGTVMSCAVMKQFAFVHMRENAGAVRAIEALHGHELRPGRALVVEM
  SRPRPLNTWKIFVGNVSAACTSQELRSLFERRGRVIECDVVKDYAFVHMEKEADAKAAIAQLNGKEVKGK
  RINVELSTKGQKKGPALAIQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFF
  GRDRSPLRRSPPRASYVAPLTAQPATYRAQPSVSLGAAYRAQPSASLGVGYRTQPMAAQAASYRAQPSVS
  LGAPYRGQLASPSSQSAAASSLGPYGGVQPSASALSTYGGQAAAASSLNSYGAQGSSLASYGNQPSSYGA
  QAASSYGVRAAASSYNTQGAASSLGSYGAQAASYGAQSAASSLAYGAQAASYSAQPSASYSAQSAPYAAQ
  QAASYSSQPAAYVAQPATAAAYASQPAAYAAQATTPMAGSYGAQPVVQTQLNSYGAQASIGLSGSYGAQS
  AAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGSAYDGTGQPSAAYLS
  MSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYGSDRRLAELSDYRRLSESQLSFRRSPTK
  SSLDYRRLPDAHSDYARYSGSYNDYLRAAQMHSGYQRRM
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019869
• ORF Size: 2007 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019869.3
• RefSeq Size: 2809 bp
• RefSeq ORF: 2010 bp
• Locus ID: 56275
• UniProt ID: Q8C2Q3
• Cytogenetics: 19 A
• MW: 69.9 kDa
• Gene Summary: May function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1 (By similarity). Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity (PubMed:25385835). Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ (By similarity). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.[UniProtKB/Swiss-Prot Function]