Avpr1b (NM_011924) Mouse Tagged ORF Clone

CAT#: MR217375

Avpr1b (Myc-DDK-tagged) - Mouse arginine vasopressin receptor 1B (Avpr1b)

Product Images

Circular map for MR217375

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Avpr1b
• Synonyms: AVPR3; V3/V1b; VIBR; VPR3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217375 representing NM_011924
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCTGAGCCTTCTTGGACTGCTACCCCCTCCCCTGGGGGCACCCTGTTTGTCCCCAATACCACCA
  CACCCTGGCTGGGTAGGGATGAAGAGCTAGCCAAGGTGGAAATTGGTATCCTAGCTACTGTCCTGGTTCT
  GGCCACAGGAGGCAACCTGGCTGTACTACTGATTCTGGGCCTCCAGGGCCATAAGCGCTCCCGCATGCAT
  CTGTTCGTGCTGCACTTGGCCCTGACCGACCTGGGCGTGGCGCTTTTCCAGGTACTGCCTCAGCTGCTCT
  GGGACATCACCTATCGCTTCCAGGGCTCTGACCTCCTCTGCCGGGCTGTCAAATATCTACAGGTGCTCAG
  CATGTTTGCTTCCACTTACATGCTGCTGGCCATGACGCTGGACCGGTACCTGGCTGTCTGTCACCCTCTT
  CGAAGCCTCCAGCAGCCCAGCCAGTCCACCTACCCTCTTATTGCTGCTCCCTGGCTGCTGGCTGCCATCC
  TCAGCCTCCCTCAAGTGTTCATTTTTTCGTTGCGAGAGGTGATCCAGGGCTCAGGGGTGCTGGACTGCTG
  GGCAGATTTCTACTTTTCCTGGGGCCCGCGGGCCTACATCACCTGGACCACCATGGCCATCTTTGTGCTG
  CCCGTGGTGGTGCTCACAGCTTGCTATGGCCTCATCTGCCACGAGATCTACAAGAACCTGAAAGTCAAGA
  CACAGGCTGGCAGGGAGGAAAGAAGGGGCTGGCCCAAGTCCTCATCTTCTGCCGCTGCTGCAGCCACTAG
  GGGGCTGCCGTCCCGGGTCAGCAGCATCAGTACCATCTCCAGGGCAAAGATCCGAACCGTGAAGATGACC
  TTTGTCATTGTGCTGGCCTACATTGCTTGCTGGGCACCTTTCTTCAGTGTCCAGATGTGGTCTGTGTGGG
  ACGAGAATGCCCCTAATGAAGATTCTACCAATGTGGCTTTCACCATCTCGATGCTTCTGGGCAACCTCAG
  CAGCTGCTGCAACCCCTGGATCTATATGGGCTTCAACAGCCACCTGTTGCCACGTTCCCTGAGTCACCGC
  GCCTGCTGCAGGGGTTCCAAGCCCCGAGTGCACAGGCAGCTCTCCAACAGCAGCCTCGCTAGCCGCCGCA
  CAACACTGCTGACCCACACCTGTGGTCCATCTACTCTCCGTCTTAGCCTTAACCTCAGCCTCCATGCAAA
  GCCTAAGCCTGCGGGCTCGCTGAAGGATTTGGAGCAGGTGGATGGAGAAGCCACTATGGAGACCAGCATC
  TCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217375 representing NM_011924
  Red=Cloning site Green=Tags(s)
  
  MDSEPSWTATPSPGGTLFVPNTTTPWLGRDEELAKVEIGILATVLVLATGGNLAVLLILGLQGHKRSRMH
  LFVLHLALTDLGVALFQVLPQLLWDITYRFQGSDLLCRAVKYLQVLSMFASTYMLLAMTLDRYLAVCHPL
  RSLQQPSQSTYPLIAAPWLLAAILSLPQVFIFSLREVIQGSGVLDCWADFYFSWGPRAYITWTTMAIFVL
  PVVVLTACYGLICHEIYKNLKVKTQAGREERRGWPKSSSSAAAAATRGLPSRVSSISTISRAKIRTVKMT
  FVIVLAYIACWAPFFSVQMWSVWDENAPNEDSTNVAFTISMLLGNLSSCCNPWIYMGFNSHLLPRSLSHR
  ACCRGSKPRVHRQLSNSSLASRRTTLLTHTCGPSTLRLSLNLSLHAKPKPAGSLKDLEQVDGEATMETSI
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011924
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011924.2, NP_036054.1
• RefSeq Size: 4148 bp
• RefSeq ORF: 1266 bp
• Locus ID: 26361
• UniProt ID: Q9WU02
• Cytogenetics: 1 E4
• MW: 47 kDa
• Gene Summary: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system.[UniProtKB/Swiss-Prot Function]