Dcun1d2 (NM_001024504) Mouse Tagged ORF Clone

CAT#: MR217177

Dcun1d2 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae) (Dcun1d2), transcript variant 1

Product Images

Circular map for MR217177

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dcun1d2
• Synonyms: AW495713; mFLJ10704
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217177 representing NM_001024504
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATAAACTTAAATCAGCTCAGAAGGACAAGGTCCGCCAGTTCATGGCATGCACTCAGGCTAGCGAGA
  GGACTGCCATCTACTGCTTGACACAGAATGAGTGGAAACTAGATGAGGCCACAGACAGTTTCTTCCAAAA
  CCCCGAGGCGTTTCACCGAGAGTCTATGAAGAGCAGTGTGGACCAGAAGAAGCTGGAGCAACTGTACAGC
  AGGTACAAAGACCCACAGGACGAAAACAAAATTGGAATCGATGGGATTCAGCAGTTCTGTGATGACCTGA
  GTCTGGACCCCGCCAGTATCAGCGTTTTGGTCATAGCGTGGAAGTTCAGGGCCGCCACTCAATGTGAATT
  TAGCAAAAAGGAATTTGTGGATGGCATGACGGAGCTGGGGTGTGATAGCACCGAGAGGCTGAAAGCTCTT
  CTCCCCAGACTGGAGCAAGAGCTTAAGGACCCAGCCAAGTTCAAGGACCTTTACCAGTTTACCTTCACCT
  TCGCTAAGAACCCTGGGCAGAAGGGCTTAGATTTAGAAATGGCTGTTGCATATTGGAAGTTAGTATTGTC
  TGGAAGGTTTAAGTTTTTAGATCTTTGGAACACATTTCTGCTGGAGCATCACAAAAGATCAATTCCAAGG
  GATACTTGGAACCTTCTCCTAGATTTTGGAAACATGATTGCAGATGATTTGTCTAACTACGATGAAGAAG
  GAGCATGGCCTGTCCTTATAGATGACTTTGTAGAATACGCACGGCCAGTGGTCACAGGCGGGAGACGCAG
  CCCTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217177 representing NM_001024504
  Red=Cloning site Green=Tags(s)
  
  MHKLKSAQKDKVRQFMACTQASERTAIYCLTQNEWKLDEATDSFFQNPEAFHRESMKSSVDQKKLEQLYS
  RYKDPQDENKIGIDGIQQFCDDLSLDPASISVLVIAWKFRAATQCEFSKKEFVDGMTELGCDSTERLKAL
  LPRLEQELKDPAKFKDLYQFTFTFAKNPGQKGLDLEMAVAYWKLVLSGRFKFLDLWNTFLLEHHKRSIPR
  DTWNLLLDFGNMIADDLSNYDEEGAWPVLIDDFVEYARPVVTGGRRSPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024504
• ORF Size: 777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024504.2, NP_001019675.2
• RefSeq Size: 3059 bp
• RefSeq ORF: 780 bp
• Locus ID: 102323
• UniProt ID: Q8BZJ7
• Cytogenetics: 8 A1.1
• MW: 30.5 kDa
• Gene Summary: Potently stimulates the neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes from the NEDD8-conjugating E2 enzyme UBC12. Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity (By similarity).[UniProtKB/Swiss-Prot Function]