Zfp287 (NM_133208) Mouse Tagged ORF Clone

CAT#: MR216499

Zfp287 (Myc-DDK-tagged) - Mouse zinc finger protein 287 (Zfp287)

Product Images

Circular map for MR216499

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp287
• Synonyms: B230333C16Rik; mszf16; mszf74; SKAT-2; Skat2; Znf287
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216499 representing NM_133208
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAATTCTTCACTTTCCCAAGTCCTTCTAATGTGGAAGCCAGGCAAGATCCAGAAGGGACCCTGCA
  GTGCTGAGCAGCAGACACTCACCTCAAGACTCTTGCGTGACACTGAAACCTGTCGACGGAATTTTAGAAA
  TTTCCCATACCCGGATGTGGCAGGCCCTCGGAAAGCGTTGTGTCAGCTCCGAGAGCTCTGCCTGAAGTGG
  CTGAGACCTGAGGTTCATTCCAAGGAACAAATTCTGGAGTTGCTGGTGCTGGAGCAATTTCTGAGCATCC
  TGCCTGGGGAGGTTAGGACTTGGGTAAATTCTCAGTACCCAGAGAGCAGCGAAGAAGTGGTGGCTTTGGT
  GGAGGATTTGACTCAGATCCTAGAAGAAGAAGAAGCTCCTCAGAGTTCTGCCCTCCCCCAAGACACCCCA
  GAGGATGACCCCAACCATGACCCCAACCCTGCTTCCCAGGCAGGGTGGCTCAGTGACGTGGTGACCAAAG
  ACTTGGTGACATTCAATGACGTGGCTGTGGACATCACCCAAGAAGACTGGGAACTGATGCCCCCTGTTCA
  GAAGGAATTGTATAAGACTGTGACTTTACAGAACTATTGGAACATGGTTTCTCTAGGACTGACTGTGTAC
  AGGCCAACTGTGATTCCCGTCTTAGAAGAGCCGTGGATGGTGATAAAAGAAATTGTAGAAGGCCCTAATC
  CAGAATGGGAACCTAAAGCTCAGGCACAGTGCCCAGCAAAGCATCTCCCTGAACTCAAGCAGGACGGAAC
  CCAAACCGTAAAACTGGAAGATTCCTATGACGACGACAACGATGACAGCGTAGAGAGTCCGCCAGTGTGT
  GCCTTCGGGATGATCCACATAGATGAGGAAGGCTTCAGTGTGAAGTCAGAGCTTTCACAAGAAGACCCTA
  CAGAAGAATACCTTAGCAAATGTGACATATATAGAGTGACTTTTGAAAAGCACACAAACCTAGGGGTCCA
  GTTTGATACCCAGTCGGATGATAAAACTGCTCTGTATAATGAAAGCAAGCCACCGTTCAGCAATGCCTCG
  TCTGGTGGTGCCGTGCGTGGGAAAATACTTCCTGGAGATAAGCCTTATTCCTGTAATGTCTGTGGGAAAC
  AGTTTAGAAAGTACCCTTCCCTCCTGGCGCACCGAGAGAACCACGCCAAAGAGAAAGCTTATGAGTGTGA
  AGAATGCGGCAAAGAATTTAAGCATCTCTCCTCCCTCATCGCACATCAGAGAATGCACACCGGAGAAAAA
  CCGTACGAATGCCACCAGTGTGGGAAAGCCTTCAGTCAGCGAGCACACCTGACTATACACCAGAGAATTC
  ACACTGGAGAGAAACCCTACAAATGTGAGGACTGTGGGAAAGACTTCAGTCAGCGCGCGCACCTCACCAT
  CCATCAAAGGACACACACTGGGGAGAAGCCGTATAAATGTCTGGAGTGCAGTAAAACCTTTAGCCATAGT
  TCATCACTGATTAATCATCAGAGAGTTCATACTGGAGAAAAACCTTATATATGCAACGAATGTGGGAAGA
  CTTTCAGTCAGAGCACGCACCTTCTCCAGCATCAGAAAATCCACACTGGGAAAAAGCCGTACAAGTGCAA
  CGAGTGTTGGAAAGTGTTCAGCCAGAGCACTTACCTCATCCGACACCAGAGAATCCATTCTGGAGAGAAG
  TGCTACAAATGCACTGCCTGTGGGAAGGCCTTTGCCCACTCCTCAACTCTCATTCAACATCAGACCACCC
  ACACCGGAGAGAAGTCCTATATATGCAACGTGTGTGGGAAAGCCTTCAGCCAAAGCGCCAACCTTACCCA
  GCATCATAGAACACACACTGGAGAGAAACCGTACAAATGTAGTGTGTGTGGAAAAGCCTTCAGCCAGAGT
  GTGCACCTCACCCAGCACCAGAGGATTCACAATGGAGAAAAGCCCTTTAAGTGTAATACCTGTGGGAAAG
  CTTACAGACAGGGTGCAAACCTGACTCAGCACCAACGGGTCCACACTGGAGAGAAGCCCTACAAATGTCA
  CCACTGCGGGAAAGCCTTTATCTACTCTTCATCCCTTAACCAACATCGAAGAACTCACACTGGAGAGCGA
  CCCTATAAGTGTAGTCACTGTAACAAAGATTTTAGCCAGAGAACATGCCTTATTCAACACCAGAGGATTC
  ACACAGGAGAAAAGCCCTACGGATGCCGTATATGTGGGAAAGCCTTCACCCAGAGTACCAATCTTATTCA
  GCATCAGCGGGTTCATACGGGTGCCAGACATCGCAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216499 representing NM_133208
  Red=Cloning site Green=Tags(s)
  
  MANSSLSQVLLMWKPGKIQKGPCSAEQQTLTSRLLRDTETCRRNFRNFPYPDVAGPRKALCQLRELCLKW
  LRPEVHSKEQILELLVLEQFLSILPGEVRTWVNSQYPESSEEVVALVEDLTQILEEEEAPQSSALPQDTP
  EDDPNHDPNPASQAGWLSDVVTKDLVTFNDVAVDITQEDWELMPPVQKELYKTVTLQNYWNMVSLGLTVY
  RPTVIPVLEEPWMVIKEIVEGPNPEWEPKAQAQCPAKHLPELKQDGTQTVKLEDSYDDDNDDSVESPPVC
  AFGMIHIDEEGFSVKSELSQEDPTEEYLSKCDIYRVTFEKHTNLGVQFDTQSDDKTALYNESKPPFSNAS
  SGGAVRGKILPGDKPYSCNVCGKQFRKYPSLLAHRENHAKEKAYECEECGKEFKHLSSLIAHQRMHTGEK
  PYECHQCGKAFSQRAHLTIHQRIHTGEKPYKCEDCGKDFSQRAHLTIHQRTHTGEKPYKCLECSKTFSHS
  SSLINHQRVHTGEKPYICNECGKTFSQSTHLLQHQKIHTGKKPYKCNECWKVFSQSTYLIRHQRIHSGEK
  CYKCTACGKAFAHSSTLIQHQTTHTGEKSYICNVCGKAFSQSANLTQHHRTHTGEKPYKCSVCGKAFSQS
  VHLTQHQRIHNGEKPFKCNTCGKAYRQGANLTQHQRVHTGEKPYKCHHCGKAFIYSSSLNQHRRTHTGER
  PYKCSHCNKDFSQRTCLIQHQRIHTGEKPYGCRICGKAFTQSTNLIQHQRVHTGARHRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133208
• ORF Size: 2277 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133208.3
• RefSeq Size: 5021 bp
• RefSeq ORF: 2280 bp
• Locus ID: 170740
• UniProt ID: Q9EQB9
• Cytogenetics: 11 B2
• MW: 86.6 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]