Hgfac (NM_019447) Mouse Tagged ORF Clone
CAT#: MR216475
- TrueORF®
Hgfac (Myc-DDK-tagged) - Mouse hepatocyte growth factor activator (Hgfac)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_019447" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Hgfac |
Synonyms | HGF; HGFA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216475 representing NM_019447
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGACAGGCCTGGATCTCTAGCCTCTGTCCCTTACCCAGGCCATGTCCCTTCCTGCTGCTCCTGC TGCTGCTGGTGGTGCCTCGGGGGGCCCAGCCCCAGGCTGGCAGGAACCACACAGAGCCCCCAGGACCTAA CGTTACAGCCACCCCCGTGACCCCCACGATCCCTGTGATCTCTGGGAATGTCAGCACCTCAACAGAAAGT GCTCCAGCAGCAGAGACTGAGGGACCCCAAAGTGAGAGGTACCCTCCTCCCTCCAGCAGCAGCCCCCCTG GGGGCCAAGTGCTCACCGAGTCTGGGCAGCCGTGCAGGTTCCCTTTCCGCTACGGTGGCCGCATGCTGCA CTCCTGTACCTCTGAGGGAAGTGCCTACAGGAAGTGGTGCGCTACAACACACAACTATGACCGAGACCGG GCCTGGGGCTACTGTGCAGAGGTGACCCTGCCTGTGGAAGGTCCAGCTATCCTTGACCCTTGTGCCTCTG GGCCCTGCCTCAATGGGGGCACATGTTCCAGTACACATGACCATGGGTCCTACCACTGCTCTTGCCCGCT GGCCTTCACAGGCAAGGACTGTGGCACAGAGAAATGCTTTGATGAAACACGCTATGAGTATTTTGAGGTG GGCGACCACTGGGCCCGTGTGAGCGAGGGACATGTGGAGCAATGTGGCTGTATGGAGGGCCAGGCCCGGT GTGAAGACACCCACCACACAGCTTGTCTGAGCAGCCCATGTCTGAACGGAGGCACCTGCCACCTGATTGT GGGCACAGGGACCAGCGTCTGCACCTGCCCGTTGGGCTATGCTGGGCGGTTCTGTAACATTGTTCCCACA GAGCACTGCTTCCTGGGAAATGGTACAGAGTACCGAGGCGTGGCCAGCACCGCTGCCTCGGGCCTGAGCT GCCTGGCCTGGAATTCTGACCTGCTCTACCAGGAGCTGCACGTGGACTCAGTGGCTGCTGCTGTCCTGCT TGGCCTGGGCCCTCACGCTTACTGCCGGAACCCAGACAAGGATGAGAGGCCTTGGTGCTATGTGGTGAAG GACAACGCACTGTCGTGGGAGTATTGCCGCCTGACAGCCTGTGAATCCCTGGCCAGAGTCCACTCCCAAA CCCCGGAGATCCTAGCAGCCCTGCCCGAGTCAGCCCCGGCTGTGCGTCCTACCTGTGGCAAGAGGCACAA GAAGAGGACGTTCTTGAGACCACGCATCATCGGGGGCTCATCATCTTTGCCTGGCTCACACCCCTGGCTG GCTGCCATCTACATTGGGAATAGCTTCTGTGCCGGGAGCCTTGTCCATACCTGCTGGGTAGTGTCTGCAG CCCACTGCTTCGCCAACAGCCCCCCCAGGGACAGCATCACAGTGGTACTGGGTCAGCACTTCTTCAACCG CACCACGGATGTGACACAGACATTTGGCATTGAGAAGTATGTGCCCTACACCCTGTACTCGGTGTTCAAC CCCAACAACCATGACCTTGTCTTGATCCGGCTAAAGAAGAAGGGAGAGCGCTGTGCTGTCCGCTCCCAGT TTGTTCAACCCATCTGCCTGCCTGAGGCAGGCAGCTCCTTCCCTACTGGACACAAGTGTCAGATTGCAGG CTGGGGCCACATGGATGAAAATGTGAGCAGCTACTCCAACTCCCTGCTGGAGGCACTGGTCCCTCTTGTT GCTGACCACAAGTGTAGCAGCCCAGAGGTATATGGTGCTGACATCAGCCCTAACATGCTCTGTGCCGGCT ACTTCGACTGCAAGTCCGATGCCTGCCAGGGGGACTCAGGTGGGCCCTTGGTCTGTGAGAAGAATGGTGT GGCTTACCTGTATGGCATCATCAGCTGGGGTGATGGCTGTGGGCGCCTCAACAAGCCAGGAGTCTACACC CGTGTGGCCAATTATGTGGACTGGATCAACGACCGTATTCGACCGCCCAAGCGACCCGTGGCTACGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216475 representing NM_019447
Red=Cloning site Green=Tags(s) MGRQAWISSLCPLPRPCPFLLLLLLLVVPRGAQPQAGRNHTEPPGPNVTATPVTPTIPVISGNVSTSTES APAAETEGPQSERYPPPSSSSPPGGQVLTESGQPCRFPFRYGGRMLHSCTSEGSAYRKWCATTHNYDRDR AWGYCAEVTLPVEGPAILDPCASGPCLNGGTCSSTHDHGSYHCSCPLAFTGKDCGTEKCFDETRYEYFEV GDHWARVSEGHVEQCGCMEGQARCEDTHHTACLSSPCLNGGTCHLIVGTGTSVCTCPLGYAGRFCNIVPT EHCFLGNGTEYRGVASTAASGLSCLAWNSDLLYQELHVDSVAAAVLLGLGPHAYCRNPDKDERPWCYVVK DNALSWEYCRLTACESLARVHSQTPEILAALPESAPAVRPTCGKRHKKRTFLRPRIIGGSSSLPGSHPWL AAIYIGNSFCAGSLVHTCWVVSAAHCFANSPPRDSITVVLGQHFFNRTTDVTQTFGIEKYVPYTLYSVFN PNNHDLVLIRLKKKGERCAVRSQFVQPICLPEAGSSFPTGHKCQIAGWGHMDENVSSYSNSLLEALVPLV ADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLVCEKNGVAYLYGIISWGDGCGRLNKPGVYT RVANYVDWINDRIRPPKRPVATS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019447 |
ORF Size | 1959 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019447.3, NP_062320.2 |
RefSeq Size | 2103 bp |
RefSeq ORF | 1962 bp |
Locus ID | 54426 |
UniProt ID | Q9R098 |
Cytogenetics | 5 B2 |
MW | 71 kDa |
Gene Summary | This gene encodes a serine protease enzyme that proteolytically activates hepatocyte growth factor (HGF) and plays a vital role in the regulation of HGF activity in the regeneration and repair of various tissues. The encoded protein is an inactive zymogen that is proteolytically activated to generate a heterodimeric enzyme consisting of a short chain and a long chain linked by a disulfide bridge. Mice lacking the encoded protein display an impairment in mucosal regeneration after injury. [provided by RefSeq, Jul 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220090 | Hgfac (untagged) - Mouse hepatocyte growth factor activator (Hgfac), (10ug) |
USD 670.00 |
|
MG216475 | Hgfac (tGFP-tagged) - Mouse hepatocyte growth factor activator (Hgfac), (10ug) |
USD 808.00 |
|
MR216475L3 | Lenti ORF clone of Hgfac (Myc-DDK-tagged) - Mouse hepatocyte growth factor activator (Hgfac) |
USD 908.00 |
|
MR216475L4 | Lenti ORF clone of Hgfac (mGFP-tagged) - Mouse hepatocyte growth factor activator (Hgfac) |
USD 908.00 |
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