Hgfac (NM_019447) Mouse Tagged ORF Clone

CAT#: MR216475

Hgfac (Myc-DDK-tagged) - Mouse hepatocyte growth factor activator (Hgfac)

Product Images

Circular map for MR216475

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hgfac
• Synonyms: HGF; HGFA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216475 representing NM_019447
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGACAGGCCTGGATCTCTAGCCTCTGTCCCTTACCCAGGCCATGTCCCTTCCTGCTGCTCCTGC
  TGCTGCTGGTGGTGCCTCGGGGGGCCCAGCCCCAGGCTGGCAGGAACCACACAGAGCCCCCAGGACCTAA
  CGTTACAGCCACCCCCGTGACCCCCACGATCCCTGTGATCTCTGGGAATGTCAGCACCTCAACAGAAAGT
  GCTCCAGCAGCAGAGACTGAGGGACCCCAAAGTGAGAGGTACCCTCCTCCCTCCAGCAGCAGCCCCCCTG
  GGGGCCAAGTGCTCACCGAGTCTGGGCAGCCGTGCAGGTTCCCTTTCCGCTACGGTGGCCGCATGCTGCA
  CTCCTGTACCTCTGAGGGAAGTGCCTACAGGAAGTGGTGCGCTACAACACACAACTATGACCGAGACCGG
  GCCTGGGGCTACTGTGCAGAGGTGACCCTGCCTGTGGAAGGTCCAGCTATCCTTGACCCTTGTGCCTCTG
  GGCCCTGCCTCAATGGGGGCACATGTTCCAGTACACATGACCATGGGTCCTACCACTGCTCTTGCCCGCT
  GGCCTTCACAGGCAAGGACTGTGGCACAGAGAAATGCTTTGATGAAACACGCTATGAGTATTTTGAGGTG
  GGCGACCACTGGGCCCGTGTGAGCGAGGGACATGTGGAGCAATGTGGCTGTATGGAGGGCCAGGCCCGGT
  GTGAAGACACCCACCACACAGCTTGTCTGAGCAGCCCATGTCTGAACGGAGGCACCTGCCACCTGATTGT
  GGGCACAGGGACCAGCGTCTGCACCTGCCCGTTGGGCTATGCTGGGCGGTTCTGTAACATTGTTCCCACA
  GAGCACTGCTTCCTGGGAAATGGTACAGAGTACCGAGGCGTGGCCAGCACCGCTGCCTCGGGCCTGAGCT
  GCCTGGCCTGGAATTCTGACCTGCTCTACCAGGAGCTGCACGTGGACTCAGTGGCTGCTGCTGTCCTGCT
  TGGCCTGGGCCCTCACGCTTACTGCCGGAACCCAGACAAGGATGAGAGGCCTTGGTGCTATGTGGTGAAG
  GACAACGCACTGTCGTGGGAGTATTGCCGCCTGACAGCCTGTGAATCCCTGGCCAGAGTCCACTCCCAAA
  CCCCGGAGATCCTAGCAGCCCTGCCCGAGTCAGCCCCGGCTGTGCGTCCTACCTGTGGCAAGAGGCACAA
  GAAGAGGACGTTCTTGAGACCACGCATCATCGGGGGCTCATCATCTTTGCCTGGCTCACACCCCTGGCTG
  GCTGCCATCTACATTGGGAATAGCTTCTGTGCCGGGAGCCTTGTCCATACCTGCTGGGTAGTGTCTGCAG
  CCCACTGCTTCGCCAACAGCCCCCCCAGGGACAGCATCACAGTGGTACTGGGTCAGCACTTCTTCAACCG
  CACCACGGATGTGACACAGACATTTGGCATTGAGAAGTATGTGCCCTACACCCTGTACTCGGTGTTCAAC
  CCCAACAACCATGACCTTGTCTTGATCCGGCTAAAGAAGAAGGGAGAGCGCTGTGCTGTCCGCTCCCAGT
  TTGTTCAACCCATCTGCCTGCCTGAGGCAGGCAGCTCCTTCCCTACTGGACACAAGTGTCAGATTGCAGG
  CTGGGGCCACATGGATGAAAATGTGAGCAGCTACTCCAACTCCCTGCTGGAGGCACTGGTCCCTCTTGTT
  GCTGACCACAAGTGTAGCAGCCCAGAGGTATATGGTGCTGACATCAGCCCTAACATGCTCTGTGCCGGCT
  ACTTCGACTGCAAGTCCGATGCCTGCCAGGGGGACTCAGGTGGGCCCTTGGTCTGTGAGAAGAATGGTGT
  GGCTTACCTGTATGGCATCATCAGCTGGGGTGATGGCTGTGGGCGCCTCAACAAGCCAGGAGTCTACACC
  CGTGTGGCCAATTATGTGGACTGGATCAACGACCGTATTCGACCGCCCAAGCGACCCGTGGCTACGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216475 representing NM_019447
  Red=Cloning site Green=Tags(s)
  
  MGRQAWISSLCPLPRPCPFLLLLLLLVVPRGAQPQAGRNHTEPPGPNVTATPVTPTIPVISGNVSTSTES
  APAAETEGPQSERYPPPSSSSPPGGQVLTESGQPCRFPFRYGGRMLHSCTSEGSAYRKWCATTHNYDRDR
  AWGYCAEVTLPVEGPAILDPCASGPCLNGGTCSSTHDHGSYHCSCPLAFTGKDCGTEKCFDETRYEYFEV
  GDHWARVSEGHVEQCGCMEGQARCEDTHHTACLSSPCLNGGTCHLIVGTGTSVCTCPLGYAGRFCNIVPT
  EHCFLGNGTEYRGVASTAASGLSCLAWNSDLLYQELHVDSVAAAVLLGLGPHAYCRNPDKDERPWCYVVK
  DNALSWEYCRLTACESLARVHSQTPEILAALPESAPAVRPTCGKRHKKRTFLRPRIIGGSSSLPGSHPWL
  AAIYIGNSFCAGSLVHTCWVVSAAHCFANSPPRDSITVVLGQHFFNRTTDVTQTFGIEKYVPYTLYSVFN
  PNNHDLVLIRLKKKGERCAVRSQFVQPICLPEAGSSFPTGHKCQIAGWGHMDENVSSYSNSLLEALVPLV
  ADHKCSSPEVYGADISPNMLCAGYFDCKSDACQGDSGGPLVCEKNGVAYLYGIISWGDGCGRLNKPGVYT
  RVANYVDWINDRIRPPKRPVATS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019447
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019447.3, NP_062320.2
• RefSeq Size: 2103 bp
• RefSeq ORF: 1962 bp
• Locus ID: 54426
• UniProt ID: Q9R098
• Cytogenetics: 5 B2
• MW: 71 kDa
• Gene Summary: This gene encodes a serine protease enzyme that proteolytically activates hepatocyte growth factor (HGF) and plays a vital role in the regulation of HGF activity in the regeneration and repair of various tissues. The encoded protein is an inactive zymogen that is proteolytically activated to generate a heterodimeric enzyme consisting of a short chain and a long chain linked by a disulfide bridge. Mice lacking the encoded protein display an impairment in mucosal regeneration after injury. [provided by RefSeq, Jul 2015]