Ly6g5c (NM_148947) Mouse Tagged ORF Clone

CAT#: MR216285

Ly6g5c (Myc-DDK-tagged) - Mouse lymphocyte antigen 6 complex, locus G5C (Ly6g5c)

Product Images

Circular map for MR216285

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ly6g5c
• Synonyms: G5c; NG33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216285 representing NM_148947
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTTATGGCAGGCCCTGCAGCCAGCTGGTCCCTGAGGCCCCTGGGACTCCATGGCGTCCCCCAAG
  CCTTGTGTGCTGTCCTCTTAACAGTGCTGGTCATGAAGACCTTGGTTCTCGGTGATACCAAGCTCGAGGA
  CCTTCACCCTCAGTCCCTCCCACTAAACAAGTACCTGAATTGCTACCGATGTCTGCTGGAGACCGAAGAG
  CTGGGGTGCCTCCTGGGGTCTGACACCTGCCTGACACCTCTGGGCAGCAGCTGTGTCACCCTGCACATAA
  AGAACAGCAGCGGTTTTAATGTCATGGTGAGCGACTGCTACAGCAAGGAGCAGATGGTCCATTGTTCATA
  TACCCGTGCTTCCCCGGTGTTTGGCTTTTGGATATTCTATCAGTGTTGCTTCCTGGATTTCTGCAACAAT
  CCGGACAACAGAAAGAATAGCATGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216285 representing NM_148947
  Red=Cloning site Green=Tags(s)
  
  MLFMAGPAASWSLRPLGLHGVPQALCAVLLTVLVMKTLVLGDTKLEDLHPQSLPLNKYLNCYRCLLETEE
  LGCLLGSDTCLTPLGSSCVTLHIKNSSGFNVMVSDCYSKEQMVHCSYTRASPVFGFWIFYQCCFLDFCNN
  PDNRKNSMH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148947
• ORF Size: 447 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148947.2
• RefSeq Size: 450 bp
• RefSeq ORF: 450 bp
• Locus ID: 114652
• UniProt ID: Q8K1T5
• Cytogenetics: 17 B1
• MW: 17 kDa
• Gene Summary: May have a role in hematopoietic cell differentiation.[UniProtKB/Swiss-Prot Function]