Ifne (NM_177348) Mouse Tagged ORF Clone
CAT#: MR216277
- TrueORF®
Ifne (Myc-DDK-tagged) - Mouse interferon epsilon (Ifne)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_177348" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ifne |
Synonyms | Ifn-tau-1; Ifne1; Ifnt1; Infe1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216277 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCACAGACAGCTCCCTGAAACGGTGTTGCTGCTCTTGGTTTCTTCCACTATCTTCTCCCTAGAAC CGAAACGGATTCCCTTCCAATTGTGGATGAACAGAGAAAGCCTACAACTACTGAAACCTTTGCCAAGCTC GTCAGTCCAGCAGTGTCTAGCACACAGGAAGAATTTCCTGCTTCCTCAGCAGCCTGTGAGTCCTCACCAG TACCAAGAGGGACAGGTGCTGGCTGTTGTGCACGAGATCCTTCAGCAGATCTTCACGCTCCTCCAGACAC ATGGGACTATGGGCATTTGGGAGGAAAACCATATAGAAAAAGTCTTAGCTGCGCTTCACCGGCAGCTGGA ATACGTGGAGTCACTGGGTGGACTGAACGCAGCGCAGAAGAGTGGGGGCTCGAGTGCGCAGAACCTTAGG TTACAGATTAAAGCATACTTCAGGAGGATCCACGATTACTTGGAAAACCAAAGGTACAGCAGCTGTGCCT GGATCATTGTCCAGACAGAAATCCACCGCTGTATGTTCTTTGTGTTCAGGTTCACAACATGGCTGAGCAG ACAAGACCCAGACCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216277 protein sequence
Red=Cloning site Green=Tags(s) MVHRQLPETVLLLLVSSTIFSLEPKRIPFQLWMNRESLQLLKPLPSSSVQQCLAHRKNFLLPQQPVSPHQ YQEGQVLAVVHEILQQIFTLLQTHGTMGIWEENHIEKVLAALHRQLEYVESLGGLNAAQKSGGSSAQNLR LQIKAYFRRIHDYLENQRYSSCAWIIVQTEIHRCMFFVFRFTTWLSRQDPDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_177348 |
ORF Size | 579 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177348.2, NP_796322.1 |
RefSeq Size | 664 bp |
RefSeq ORF | 579 bp |
Locus ID | 230405 |
UniProt ID | Q80ZF2 |
Cytogenetics | 4 42.04 cM |
MW | 22.4 kDa |
Gene Summary | Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral, including HSV-2, and bacterial, including Chlamydia muridarum, genital infections.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212882 | Ifne (untagged) - Mouse interferon epsilon (Ifne), (10ug) |
USD 300.00 |
|
MG216277 | Ifne (tGFP-tagged) - Mouse interferon epsilon (Ifne), (10ug) |
USD 500.00 |
|
MR216277L3 | Lenti ORF clone of Ifne (Myc-DDK-tagged) - Mouse interferon epsilon (Ifne) |
USD 600.00 |
|
MR216277L4 | Lenti ORF clone of Ifne (mGFP-tagged) - Mouse interferon epsilon (Ifne) |
USD 600.00 |
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