Rnf20 (NM_182999) Mouse Tagged ORF Clone

CAT#: MR216271

Rnf20 (Myc-DDK-tagged) - Mouse ring finger protein 20 (Rnf20), transcript variant 2

Product Images

Circular map for MR216271

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnf20
• Synonyms: 4833430L21Rik; AW540162; C79397; mKIAA4116
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216271 representing NM_182999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGAATTGGAAATAAAAGAGCAGCTGGAGAGCCGGGCACATCTATGCCTCCAGAGAAGAAGACAG
  CTGTTGAAGATTCAGGGACCACCGTGGAAACAATTAAGTTAGGAGGTGTCTCTTCAACGGAGGAATTAGA
  CATTCGAACACTGCAATCCAAGAATCGTAAGCTAGCAGAAATGCTAGATCAGCGGCAGGCCATTGAAGAT
  GAGCTTCGGGAACACATTGAAAAGCTGGAGCGACGCCAGGCCACTGATGATGCCTCACTGTTGATTGTGA
  ACCGGTACTGGAGCCAGTTTGATGAAAACATCCGCATCATCCTTAAACGTTATGATCTGGACCAGGGTTT
  GGGGGACCTACTCACAGAAAGGAAAGCCTTGGTTGTGCCAGAACCAGAACCTGACTCTGATAGCAATCAG
  GAACGCAAAGATGACCGTGAGAGAGGGGATGGGCAAGAGCCAGCTTTCTCTTTCCTTGCTACTTTGGCTA
  GCAGTTCCAGTGAAGAGATGGAATCTCAGCTTCAGGAGCGTGTAGAGTCCTCCCGCCGTGCTGTGTCCCA
  GATTGTGACTGTCTATGATAAATTGCAAGAAAAAGTGGATCTCTTATCCCGGAAACTGAACAGTGGAGAT
  AACCTGATAGTGGAGGAAGCAGTGCAGGAGTTGAACTCCTTCCTTGCACAAGAGAATGTGAGGCTTCAGG
  AGCTGACAGACCTCCTTCAGGAGAAGCATCACACCATGTCTCAGGAGTTCTGTAAGTTGCAGGGTAAAGT
  AGAGACAGCTGAGTCACGAGTGTCCGTCTTGGAGTCCATGATTGATGACCTGCAGTGGGATATTGACAAA
  ATTCGAAAAAGAGAACAGCGTCTCAACCGGCATTTGGCTGAAGTCCTAGAGCGGGTGAATTCAAAAGGCT
  ATAAAGTATATGGAGCGGGGAGCAGTCTCTATGGAGGCACAATCACCATCAATGCCCGGAAGTTTGAGGA
  AATGAATGCAGAGCTTGAGGAGAACAAAGAGTTGGCTCAGAACCGACATTGTGAGCTGGAGAAGCTTCGT
  CAAGACTTTGAAGAGGTCACTACACAGAATGAAAAGTTGAAGGTGGAATTGCGGAGTGCAGTGGAGGAAG
  TGGTTAAGGAAACCCCAGAGTATCGCTGCATGCAGTCACAGTTCTCTGTCCTGTATAACGAGAGTCTGCA
  GTTGAAAGCCCACTTGGATGAAGCTCGGACCCTGCTTCATGGCACCCGGGGTACTCACCAGCGCCAGGTT
  GAACTCATTGAGCGAGATGAGGTTAGTCTGCATAAGAAGCTGAGAACTGAAGTCATTCAGCTGGAGGATA
  CCTTGGCCCAGGTTCGCAAGGAGTATGAGATGTTGAGGATAGAGTTTGAGCAGACCCTTGCTGCCAATGA
  ACAAGCAGGCCCCATAAACCGGGAGATGCGGCATCTCATTAGCAGCCTCCAGAATCATAACCACCAGCTG
  AAGGGTGAAGTCCTAAGGTATAAACGGAAGCTGAGAGAGGCGCAGTCTGACCTGAACAAGACACGTCTGC
  GCAGTGGCAGTGCCCTCCTGCAGTCTCAGTCTAGTACTGAGGACCCCAAGGACGAGCCCACAGAGCTGAA
  GCAGGATTCTGAAGACCTAGCCACTCATTCCTCAGCGCTGAAGGCATCTCAGGAGGATGAAGTTAAGTCC
  AAACGGGATGAAGAAGAGAGAGAACGAGAAAGGAGGGAGAAAGAAAGAGAGCGAGAGCGAGAACGAGAAA
  AGGAAAAGGAGAGAGAACGAGAGAAACAGAAACTGAAAGAGTCAGAAAAGGAGAGAGATTCTGTTAAGGA
  TAAAGAGAAAGGGAAACATGATGATGGAAGGAAGAAGGAAGCAGAAATTATCAAGCAGCTGAAGATTGAA
  CTTAAGAAAGCACAAGAGAGCCAGAAGGAGATGAAACTACTGCTGGATATGTACCGCTCTGCCCCCAAGG
  AGCAGAGAGACAAAGTGCAGCTAATGGCAGCTGAGAAGAAGTCAAAGGCAGAGTTGGAGGATCTCAGACA
  AAGACTCAAGGATCTGGAAGATAAGGAGAAGAAAGAGAACAAGAAAATGGCTGATGAGGATGCCTTGAGG
  AAGATCCGGGCAGTGGAGGAGCAGATTGAATACCTGCAGAAGAAGCTGGCTATGGCCAAGCAGGAGGAAG
  AGGCTCTCCTGTCTGAGATGGATGTCACAGGACAGGCCTTTGAGGACATGCAGGAGCAGAACATCCGTCT
  GATGCAGCAGTTGCGGGAGAAGGACGATGCAAACTTCAAGCTCATGTCAGAGCGAATCAAGTCTAATCAG
  ATCCATAAGTTGCTTAAAGAGGAAAAGGAAGAGTTAGCTGATCAGGTTTTGACACTGAAGACTCAGGTAG
  ATGCCCAGTTACAGGTAGTAAGAAAACTGGAAGAGAAGGAGCACCTACTTCAGAGTAACATTGGCACAGG
  GGAGAAGGAGCTGGGTCTTAGGACTCAAGCCTTGGAGATGAATAAACGCAAGGCAATGGAGGCGGCCCAG
  CTTGCAGATGACCTCAAAGCACAGCTAGAACTGGCTCAGAAGAAGCTCCATGATTTCCAGGATGAAATCG
  TGGAGAACAGTGTCACCAAAGAAAAGGACTTGTTCAATTTCAAACGAGCACAGGAAGACATCTCTAGGCT
  TCGAAGAAAGCTAGAGACCACCAAGAAGCCAGACAATGTGCCCAAGTGTGATGAAATTCTGATGGAGGAG
  ATAAAGGACTACAAGGCACGGCTGACCTGCCCTTGCTGTAACATGCGTAAAAAGGATGCAGTACTTACCA
  AGTGTTTTCACGTTTTCTGCTTTGAGTGTGTGAAGACGCGCTATGACACTCGCCAGCGCAAGTGTCCCAA
  GTGTAATGCTGCTTTTGGTGCCAATGATTTCCATCGCATCTACATTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216271 representing NM_182999
  Red=Cloning site Green=Tags(s)
  
  MSGIGNKRAAGEPGTSMPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQSKNRKLAEMLDQRQAIED
  ELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLDQGLGDLLTERKALVVPEPEPDSDSNQ
  ERKDDRERGDGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVDLLSRKLNSGD
  NLIVEEAVQELNSFLAQENVRLQELTDLLQEKHHTMSQEFCKLQGKVETAESRVSVLESMIDDLQWDIDK
  IRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLR
  QDFEEVTTQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQV
  ELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQL
  KGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPTELKQDSEDLATHSSALKASQEDEVKS
  KRDEEERERERREKEREREREREKEKEREREKQKLKESEKERDSVKDKEKGKHDDGRKKEAEIIKQLKIE
  LKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALR
  KIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQ
  IHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQ
  LADDLKAQLELAQKKLHDFQDEIVENSVTKEKDLFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEE
  IKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182999
• ORF Size: 2919 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182999.2, NP_892044.1
• RefSeq Size: 4159 bp
• RefSeq ORF: 2922 bp
• Locus ID: 109331
• UniProt ID: Q5DTM8
• Cytogenetics: 4 B1
• MW: 113.5 kDa
• Gene Summary: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of PA2G4 leading to its proteasome-mediated degradation.[UniProtKB/Swiss-Prot Function]