Tmem165 (NM_011626) Mouse Tagged ORF Clone

CAT#: MR216105

Tmem165 (Myc-DDK-tagged) - Mouse transmembrane protein 165 (Tmem165)

Product Images

Circular map for MR216105

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem165
• Synonyms: AV026557; pFT27; Tpardl; Tparl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216105 representing NM_011626
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCGGCCGCGGCCCGGGGGAGCGGCCGGGCGCCGACGCGAAGGCTGCTCGTGCTCTTGCTGCTTCAGT
  TGCTCTGGGCCCCGGCCGGGGTCCGCGCCGGCCCGGAGGAAGACCTGAGCCATCGGAACCAGGAGCCGCC
  GGCGCCCGCCCAGCAGCTGCAGCCGCAGCCCGCGGCGGTGCAGGGCCTCGAGCCGGCCCGGGCCGAGAAA
  GGATTGACACCAGTCGCCCCAGTTCATACCAACAAAGAAGATGCAGCTGCCCAGACGAATCTGGGATTTA
  TCCATGCGTTTGTGGCTGCCATATCAGTCATCATAGTGTCCGAACTCGGCGACAAGACGTTTTTCATAGC
  TGCCATCATGGCGATGCGCTATAACCGGCTGACTGTGCTGGCCGGGGCCATGCTGGCCTTGGCCTTGATG
  ACATGCTTGTCGGTTTTGTTTGGCTATGCCACAACAGTCATCCCCAGGGTGTATACATACTATGTTTCAA
  CTGCACTCTTCGCCATTTTTGGCATTAGAATGCTTCGGGAAGGTTTGAAGATGAGCCCAGATGAGGGTCA
  GGAGGAGCTAGAAGAAGTCCAAGCAGAGTTAAAGAAGAAGGATGAAGAATTCCAACGAACCAAACTCTTA
  AATGGGCCAGATGTTGAAACTGGTACAAGCACAGCAATACCTCAGAAAAAGTGGTTACATTTTATTTCAC
  CCATTTTTGTTCAAGCGCTCACATTAACGTTCTTGGCAGAATGGGGCGATCGCTCTCAACTCACTACCAT
  TGTCCTGGCAGCTAGAGAGGACCCTTATGGTGTAGCAGTGGGTGGCACAGTGGGACACTGCTTATGTACT
  GGATTGGCAGTAATTGGAGGAAGGATGATAGCGCAAAAGATCTCTGTCCGAACTGTGACAATCATCGGAG
  GCATCGTATTTTTGGCGTTTGCATTTTCTGCCCTGTTTATAAGTCCAGAGTCTGGTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216105 representing NM_011626
  Red=Cloning site Green=Tags(s)
  
  MAAAARGSGRAPTRRLLVLLLLQLLWAPAGVRAGPEEDLSHRNQEPPAPAQQLQPQPAAVQGLEPARAEK
  GLTPVAPVHTNKEDAAAQTNLGFIHAFVAAISVIIVSELGDKTFFIAAIMAMRYNRLTVLAGAMLALALM
  TCLSVLFGYATTVIPRVYTYYVSTALFAIFGIRMLREGLKMSPDEGQEELEEVQAELKKKDEEFQRTKLL
  NGPDVETGTSTAIPQKKWLHFISPIFVQALTLTFLAEWGDRSQLTTIVLAAREDPYGVAVGGTVGHCLCT
  GLAVIGGRMIAQKISVRTVTIIGGIVFLAFAFSALFISPESGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011626
• ORF Size: 969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011626.2, NP_035756.2
• RefSeq Size: 1896 bp
• RefSeq ORF: 972 bp
• Locus ID: 21982
• UniProt ID: P52875
• Cytogenetics: 5 40.56 cM
• MW: 35.2 kDa
• Gene Summary: May function as a calcium/proton transporter involved in calcium and in lysosomal pH homeostasis. Therefore, it may play an indirect role in protein glycosylation (By similarity).[UniProtKB/Swiss-Prot Function]