Med7 (NM_001104530) Mouse Tagged ORF Clone
CAT#: MR215853
- TrueORF®
Med7 (Myc-DDK-tagged) - Mouse mediator complex subunit 7 (Med7), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001104530" in other vectors (3)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Med7 |
Synonyms | 1110063B05Rik; Crsp9; Crsp33 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215853 representing NM_001104530
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGAACCACAGCAAGTGAGTGCACTTCCACCGCCTCCAATGCAGTACATCAAGGAATATACAGATG AAAATAGTCAGGAAGGCTTGGCTCCAGGCCGACCTCCTCCAATTAAAGACAGCTACATGATGTTTGGCAA CCAGAAGCGATGTGATGATCTTATCATTCGCCCTTTAGAAAGTCAGGGCATTGAACGGCTTCATCCTATG CAATTTGATCATAAGAAAGAGCTGAGAAAACTCAATATGTCTATACTGATTAATTTCTTAGACCTTTTAG ACATCTTAATAAGAAGTCCTGGGAGTATAAAACGAGAAGAAAAGCTAGAAGATCTTAAGCTACTTTTTGT ACATGTACATCATCTTATAAATGAATATCGACCCCACCAAGCAAGAGAGACTTTGAGAGTCATGATGGAG GTCCAGAAACGCCAGCGCCTTGAAACAGCTGAGAGGTTTCAGAAACATCTGGAACGAGTCATTGAAATGA TTCAGAATTGCTTGGCTTCTTTGCCCGATGATTTGCCCCATTCAGAAGCAGGGATGAGAGTTAAAGCTGA GCCGATGGATACTGATGATAACAGCAATTGTCCTGGACAGAATGAACAACAAAGAGAAAGTTCAGGTCAC AGAAGGGACCAGATTATAGAAAAAGATGCTGCCTTATGTGTGCTAATTGATGAAATGAATGAAAGGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215853 representing NM_001104530
Red=Cloning site Green=Tags(s) MGEPQQVSALPPPPMQYIKEYTDENSQEGLAPGRPPPIKDSYMMFGNQKRCDDLIIRPLESQGIERLHPM QFDHKKELRKLNMSILINFLDLLDILIRSPGSIKREEKLEDLKLLFVHVHHLINEYRPHQARETLRVMME VQKRQRLETAERFQKHLERVIEMIQNCLASLPDDLPHSEAGMRVKAEPMDTDDNSNCPGQNEQQRESSGH RRDQIIEKDAALCVLIDEMNERP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001104530 |
ORF Size | 699 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001104530.1, NP_001098000.1 |
RefSeq Size | 2392 bp |
RefSeq ORF | 702 bp |
Locus ID | 66213 |
UniProt ID | Q9CZB6 |
Cytogenetics | 11 B1.1 |
MW | 27.7 kDa |
Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG215853 | Med7 (tGFP-tagged) - Mouse mediator complex subunit 7 (Med7) transcript variant 2, (10ug) |
USD 530.00 |
|
MR215853L3 | Lenti ORF clone of Med7 (Myc-DDK-tagged) - Mouse mediator complex subunit 7 (Med7), transcript variant 2 |
USD 630.00 |
|
MR215853L4 | Lenti ORF clone of Med7 (mGFP-tagged) - Mouse mediator complex subunit 7 (Med7), transcript variant 2 |
USD 630.00 |
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