Amigo2 (NM_001164563) Mouse Tagged ORF Clone
CAT#: MR215778
- TrueORF®
Amigo2 (Myc-DDK-tagged) - Mouse adhesion molecule with Ig like domain 2 (Amigo2), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001164563" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Amigo2 |
Synonyms | AI415330; Ali1; AMIGO-2; AW208913 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215778 representing NM_001164563
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTTAAGGTTCCACACACTGCCCACCCTGCCTAGAGCTGTCAAACCGGGTTGCAGAGAGCTGCTGT GTCTGTTGGTGATCGCAGTGATGGTGAGCCCCAGCGCCTCAGGAATGTGCCCCACTGCTTGCATCTGTGC CACCGACATTGTCAGCTGCACCAACAAAAACCTATCTAAGGTGCCCGGGAACCTTTTCAGACTGATTAAA AGACTGGATCTGAGCTATAACAGAATCGGACTGTTGGATGCCGACTGGATCCCGGTGTCGTTTGTCAAGC TGAGCACCTTAATTCTTCGCCACAACAACATCACCAGCATCTCCACGGGCAGTTTCTCCACAACCCCAAA TTTAAAGTGTCTGGACTTATCATCCAATAGGCTGAAGTCGGTAAAGAGTGCCACATTCCAAGAGCTGAAG GCTCTGGAAGTACTGCTGCTGTACAACAACCACATTTCCTATCTGGACCCCGCAGCGTTCGGGGGGCTTT CCCACTTGCAGAAACTCTATCTGAGTGGGAACTTTCTCACACAGTTCCCTATGGATTTGTATACTGGGAG GTTCAAGCTGGCTGATCTGACATTTTTAGATGTTTCCTATAATCGAATCCCTTCCATACCGATGCACCAT ATAAACTTAGTGCCGGGGAGACAGCTGAGAGGCATCTACCTTCACGGGAACCCATTTGTATGTGACTGTT CTCTGTACTCGTTGCTGATCTTTTGGTACCGTAGGCACTTTAGCTCCGTGATGGATTTTAAGAATGACTA TACCTGTCGCCTGTGGTCTGACTCCAGGCACTCCCACCAGCTGCAGCTGCTCCAGGAGAGCTTTCTGAAC TGTTCTTACAGCGTTATCAACGGCTCCTTCCACGCACTTGGCTTTATCCACGAGGCTCAGGTTGGGGAGA GGGCGATCGTCCACTGTGACAGCAAGACTGGCAATGGAAATACTGATTTCATCTGGGTCGGTCCCGATAA CAGGCTGCTGGAGCCAGATAAAGACATGGGGAACTTTCGTGTGTTTTACAACGGAAGTCTGGTCATAGAG AACCCTGGCTTTGAGGACGCCGGGGTATATTCTTGTATCGCAATGAACAGGCAGCGGCTGTTAAACGAGA CGGTGGATATCATGATCAACGTGAGCAATTTCACCATAAACAGATCCCACGCCCACGAGGCGTTTAACAC GGCCTTTACCACCCTGGCTGCCTGCGTGGCCAGTATAGTTCTAGTGCTACTGTATCTGTACCTGACGCCG TGCCCATGCAAATGCAAAGCCAAGAGACAGAAAAACACGCTGAGCCAAAGCAGTGCCCACTCGTCCATTC TCAGTCCTGGCCCCACTGGCGATGCCTCTGCTGACGATCGGAAGGCAGGTAAAAGAGTCGTGTTTCTGGA GCCCCTGAAGGACACGGCGGCCGGACAGAATGGCAAAGTCAAGCTTTTCCCCAGTGAGACCGTTATAGCC GAGGGCATCTTAAAGTCCACCAGGGCAAAGTCTGACTCAGACTCAGTCAATTCCGTGTTCTCAGACACAC CCTTTGTGGCATCCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215778 representing NM_001164563
Red=Cloning site Green=Tags(s) MSLRFHTLPTLPRAVKPGCRELLCLLVIAVMVSPSASGMCPTACICATDIVSCTNKNLSKVPGNLFRLIK RLDLSYNRIGLLDADWIPVSFVKLSTLILRHNNITSISTGSFSTTPNLKCLDLSSNRLKSVKSATFQELK ALEVLLLYNNHISYLDPAAFGGLSHLQKLYLSGNFLTQFPMDLYTGRFKLADLTFLDVSYNRIPSIPMHH INLVPGRQLRGIYLHGNPFVCDCSLYSLLIFWYRRHFSSVMDFKNDYTCRLWSDSRHSHQLQLLQESFLN CSYSVINGSFHALGFIHEAQVGERAIVHCDSKTGNGNTDFIWVGPDNRLLEPDKDMGNFRVFYNGSLVIE NPGFEDAGVYSCIAMNRQRLLNETVDIMINVSNFTINRSHAHEAFNTAFTTLAACVASIVLVLLYLYLTP CPCKCKAKRQKNTLSQSSAHSSILSPGPTGDASADDRKAGKRVVFLEPLKDTAAGQNGKVKLFPSETVIA EGILKSTRAKSDSDSVNSVFSDTPFVAST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001164563 |
ORF Size | 1557 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001164563.1, NP_001158035.1 |
RefSeq Size | 2871 bp |
RefSeq ORF | 1560 bp |
Locus ID | 105827 |
UniProt ID | Q80ZD9 |
Cytogenetics | 15 F1 |
MW | 57.6 kDa |
Gene Summary | Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217342 | Amigo2 (untagged) - Mouse adhesion molecule with Ig like domain 2 (Amigo2), transcript variant 2, (10ug) |
USD 532.00 |
|
MG215778 | Amigo2 (tGFP-tagged) - Mouse adhesion molecule with Ig like domain 2 (Amigo2) transcript variant 2, (10ug) |
USD 683.00 |
|
MR215778L3 | Lenti ORF clone of Amigo2 (Myc-DDK-tagged) - Mouse adhesion molecule with Ig like domain 2 (Amigo2), transcript variant 2 |
USD 783.00 |
|
MR215778L4 | Lenti ORF clone of Amigo2 (mGFP-tagged) - Mouse adhesion molecule with Ig like domain 2 (Amigo2), transcript variant 2 |
USD 783.00 |
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