Ckap4 (NM_175451) Mouse Tagged ORF Clone

CAT#: MR215622

Ckap4 (Myc-DDK-tagged) - Mouse cytoskeleton-associated protein 4 (Ckap4)

Product Images

Circular map for MR215622

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ckap4
• Synonyms: 5630400A09Rik; CLIMP-63; P63
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215622 representing NM_175451
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGGCCAAACAAAGGGGCTCCAAGGGCGGCCACGGCGCCGCGAGCCCCTCGGACAAGGGCGCCC
  ACCCGTCGGGCGGCGCGGATGACGTGGCGAAGAAGCCGCCGGCGGCGCCGCAGCAGCCGCAGCCTCCCGC
  GCCGCACCCGCCGCAGCACCCGCAGAACCAGGCGCACCGGGGCGGCCACCGCGGCCGGTCCTCAGCCGCC
  ACCGCCAACGCGTCCTCCGCGTCCTGCTCGCGCAGGCTTGGCCGGGTGCTCAACTTTCTCTTCTACCTCT
  CCCTGGTGGCGGCGGCCGCCTTCTCGGGCTGGTATGTCCATCACGTCCTGGAGGAGGTCCAGCAGGTCCG
  GCGTGGCCACCAGGACTTCTCCCGTCAGAGGGATGAGCTGGGCCAGGGCTTGCAGGGCGTCGAGCAGAAG
  GTCCAATCCCTGCAAGCCACATTTGGGACTTTCGAGTCCCTCCTGAGAAACTCCCAGCATAAACAGGATC
  TCACAGAGAAAGCCGTGAAGGAAGGGGAGAGCGAGCTCAACCGAATTAGCGAAGTTCTACAGAAGTTGCA
  GAATGAGATTCTCAAAGATCTGTCGGACGGGATCCACGTGGTGAAGGATGCCCGGGAGCGGGACTTCACG
  TCCCTGGAGAACACAGTCGAGGAGCGCCTGACGGAGCTTACCAAGTCTATCAATGACAACATCGCCATCT
  TCACTGATGTCCAGAAGAGGAGCCAGAAGGAGATCAATGAGGTCAAGATGAAGGTGGCCTCCCTGGAAGA
  ATCCAAGGGGGATCGCAGTCAGGACGTGAAAACTCTGAAGGACGCCGTGAAGGAGGTCCAGGCCTCCATG
  ATGTCGAGGGAGAGGGACATTGAGGCCCTGAAGAGTTCCCTTCAGACGATGGAGTCCGACGTCTACACAG
  AGGTGCGGGAACTGGTGAGCCTCAAGCAGGAGCAACAGGCGTTCAAGCAGGCGGCCGACTCAGAGCGCCT
  GGCTCTGCAGGCCCTCACGGAGAAGCTTCTGCGG[AT]CCGAGGAGTCCTCCTCTCGTCTCCCAGAGGAC
  ATCCGGAGACTGGAGGAAGAGCTACAGCAGCTGAAGGTCGGGGCCCATGGGTCAGAGGAAGGTGCCGTCT
  TCAAAGACTCCAAAGCCTTAGAGGAACTGCAGAGGCAGATTGAGGGCCTGGGTGCCAGGCTCCAGTATGT
  AGAGGACGGGGTCTACTCAATGCAGGTGGCTTCTGCACGCCACACCGAAAGCCTGGAGTCGCTCCTGTCC
  AAGAGCCAGGAGTACGAGCAACGCCTGGCTATGCTGCAGGAGCACGTGGGAAACCTGGGCTCCTCATCTG
  ACCTGGCCAGCACAGTCAGGAGCCTGGGGGAGACCCAGCTGGCGCTGTCCAGCGACTTGAAGGAGCTGAA
  GCAGAGCCTGGGTGAGCTGCCCGGTACCGTGGAGTCACTGCAGGAGCAAGTGCTCTCACTGCTCAGCCAG
  GACCAAGCCCAGGCCGAGGGCTTGCCTCCTCAGGACTTCCTGGACAGACTGTCCTCTCTAGACAACCTGA
  AATCCTCAGTGAGCCAAGTGGAGTCAGACTTGAAAATGCTCAGGACTGCCGTGGACAGTTTGGTGGCCTA
  TTCCGTCAAAATCGAAACGAATGAGAATAACTTAGAGTCAGCCAAGGGCTTGCTGGATGACCTGCGGAAC
  GACCTGGATAGGTTGTTTCTGAAAGTTGAGAAGATCCATGAAAAGATC
  
  CTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG
  ATAAGGTTTAA
  Protein Sequence

  >MR215622 representing NM_175451
  Red=Cloning site Green=Tags(s)
  
  MPSAKQRGSKGGHGAASPSDKGAHPSGGADDVAKKPPAAPQQPQPPAPHPPQHPQNQAHRGGHRGRSSAA
  TANASSASCSRRLGRVLNFLFYLSLVAAAAFSGWYVHHVLEEVQQVRRGHQDFSRQRDELGQGLQGVEQK
  VQSLQATFGTFESLLRNSQHKQDLTEKAVKEGESELNRISEVLQKLQNEILKDLSDGIHVVKDARERDFT
  SLENTVEERLTELTKSINDNIAIFTDVQKRSQKEINEVKMKVASLEESKGDRSQDVKTLKDAVKEVQASM
  MSRERDIEALKSSLQTMESDVYTEVRELVSLKQEQQAFKQAADSERLALQALTEKLLRXXEESSSRLPED
  IRRLEEELQQLKVGAHGSEEGAVFKDSKALEELQRQIEGLGARLQYVEDGVYSMQVASARHTESLESLLS
  KSQEYEQRLAMLQEHVGNLGSSSDLASTVRSLGETQLALSSDLKELKQSLGELPGTVESLQEQVLSLLSQ
  DQAQAEGLPPQDFLDRLSSLDNLKSSVSQVESDLKMLRTAVDSLVAYSVKIETNENNLESAKGLLDDLRN
  DLDRLFLKVEKIHEKI
  
  LEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175451
• ORF Size: 1728 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175451.1, NP_780660.1
• RefSeq Size: 2915 bp
• RefSeq ORF: 1728 bp
• Locus ID: 216197
• UniProt ID: Q8BMK4
• Cytogenetics: 10 C1
• MW: 64.1 kDa
• Gene Summary: High-affinity epithelial cell surface receptor for APF.[UniProtKB/Swiss-Prot Function]