Gse1 (NM_001145896) Mouse Tagged ORF Clone

CAT#: MR215292

Gse1 (Myc-DDK-tagged) - Mouse genetic suppressor element 1 (Gse1), transcript variant 2

Product Images

Circular map for MR215292

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gse1
• Synonyms: 2210013I18Rik; mKIAA0182
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215292 representing NM_001145896
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGGCATGAGCCATGAGCCCAAGTCCCCTTCCATAGGGATGCTTTCCACGGCGACCAGGACCACCG
  CCACCGTCAACCCCCTCACCCCCTCGCCACTCAATGGCGCCCTGGTGCCCACCGGTAGCCCGGCCACCAG
  CAGTACGCTGTCGGCCCAGGCCGCTCCATCCTCCAGCTTTGCTGCCGCACTGCGCAAGCTCGCCAAACAG
  GCAGAGGAGCCCAGAGGGTCCTCACTGAGCAGCGAGTCATCTCCAGTGTCCTCTCCAGCCACCAACCATA
  GCTCTCCAGCTAGCACGCCCAAACGAGTGCCCATGGGCCCCATCATCGTCCCCCCCGGGGGTCACAGTGT
  CCCCAGCACTCCCCCAGTGGTCACTATTGCCCCCACTAAAACCGTCAATGGAGTCTGGAGAAGCGAGAGC
  CGCCAGGACTCTGGCTCACGAGGCAGCAGCAGTGGTCGGGAGCGCCTATTGGTGGAGCCACCTCTCGCCC
  AGGAAAAGGCTGCGGGCCCCGCCATCCCCTCCCACCTACTCAGCACCCCGTACCCCTTTGGCCTTTCCCC
  CGGCTCAGTTGTACAGGACTCCCGCTTCCAACCACTCAACCTCCAGCGGCCTGTGCACCATGTGGTGCCC
  CCCAGCACGGTGACTGAGGACTACTTGAGAAGCTTCCGGCCCTACCACACTGCTGAAGACTTACGCATGT
  CCTCCTTGCCTCCGCTTGGCCTGGACCCAGCCACCGCCGCAGCCTACTATCACCCCAGCTACCTGGCCCC
  ACACCCATTCCCCCACCCGGCCTTCAGGATGGATGACTCCTATTGCCTGTCAGCCTTAAGGTCTCCGTTC
  TACCCCATCCCCACCCCTGGCTCCCTGCCTCCACTGCATCCGTCGGCGATGCACCTGCATCTCTCCGGCG
  TACGGTATCCTCCTGAGCTCTCCCACTCGTCCCTGGCGGCGCTACACTCGGAGCGGATGTCCAGCCTCAG
  TGCAGAGAGGTTGCAAATGGACGAGGAACTGCGGCGGGAGAGAGAGCGAGAGCGGGAACGGGAACGGGAG
  GCCGACCGGGAGCGGGAGAAGGAGCGGGAGCGGGAGCAGCGGGAGAAGGAGCGGGAGAAGGAGCTGGAGC
  GCGAGCGGGAGAAGGAACGGGAGCGCGAGCTGGAGCGGCAGCGGGAGCAGCGGGCGAGGGAGAAGGAGCT
  GCTGGCTGCCAAGGCCTTAGAGCCCACCACCTTCCTGCCTGTGGCCGAGCTGCACGGACTCCGAGGTCAC
  AGCACGGAGGAGCGGCCCAAGCCCTCGGAGCAGCTGACCCCAACCCGAGCAGAGAAGCTGAAGGACGTGG
  GCTTGCAGGCGCCCAAGCCCGTGCAGCACCCTCTGCACCCAGTGCCTGCCCCACACCACACGGTGCCCAG
  CCTCATCTCCAGCCACGGCATCTTCTCTCTGCCTGGAAGCAGCGCCACCACCGCCCTGCTGATCCAGCGC
  ACCAACGAGGAGGAGAAGTGGCTGGCACGGCAGCGCCGCCTGCGGCAGGAGAAGGAAGACCGCCAGTCCC
  AGGTGTCCGAGTTCCGGCAGCAGGTCCTGGAGCAGCACCTAGACCTGGGCCGGCCCCTGGTGCCCACAGA
  GGCGGAGCACAGGCCCGAGAGCACCAGGCCAGGAACAAACCGGCATGAGCAGGGCAGCCGTGAACCCCCA
  CAGCACTTTGGTGGCCCTCCACCCCTCATCTCACCCAAGCCCCAGCAGCACACCGTACCCACAGCCCTCT
  GGAACCCAGTGTCTCTGATGGACAATGCTCTGGAGACGCGGCGGGCCGAGAGCCACTCTCTGCATAGCCA
  CCCGACTGCTTTTGAGCCCAGCCGCCAGGCCGCCGTGCCACTGGTGAAGGTGGAGCGAGTTTATTGCTCA
  GAGAAGGCAGAGGAGCCCCGGAAGCGTGAGGCCACACCACTGGACAAATACCAGCCACCACCACCACCAC
  CACCACCGCGGGAGGCAGGAAGTCTGGAGCCTCAGACCTTTCCCCACGGACCTGGGCCTTTCCTTACTGA
  ACTTGAAAAGTCAACACAGACCATCTTGGGCCAGCAGCGGCCCTCTCTTTCCCAGGCAACCTCCTTCGGG
  GAGCTCAGTGGGCCTCTGAAACCAGGCTCTCCGTACTGCCACCCCACAGCGAGGGGCCCCGATCCAGCCT
  ACATCTACGATGAGTTTCTTCAGCAGCGACGGAAGCTGGTCAGCAAACTGGACCTGGAGGAACGCAGGCG
  GCGCGAGGCCCAGGAGAAAGGGTACTACTACGACCTCGATGACTCGTACGACGAGAGTGACGAGGAGGAG
  GTCAGGGCACACCTCCGCTGTGTGGCTGAGCAGCCGCCCCTCAAACTGGACACATCCTCGGAGAAGCTAG
  AGTTCTTGCAACTTTTTGGCTTGACCACCCAACAGCAGAAGGAGGAACTGGTGGCGCAGAAGCGCCGGAA
  GCGGAGGCGGATGCTCAGAGAGAGAAGCCCGTCGCCACCTGCCGTTCAGTGCAAGCGACAGACGCCTTCC
  CCCAGGCTGGCTCTGTCCACCCGCTACAGCCCCGACGAGATGAACAACAGCCCCAACTTTGAGGAGAAGA
  GGAAGTTCCTGACCTTCTTCAACTTGACGCACATCAGTGCGGAGAAGAGGAAAGACAAAGAGAGGCTTGT
  TGAAATGCTCCGAGCCATGAAGCAGAGAGCACTGTCCGCAGCAGACTCAGTGACAAACTCTTCGAGGGAC
  AGTCCTCCCGTCTCCCTGAGTGAACCAGCCACACAGCCAGCTCCTCTAGAGACGGATCAGCCTGTCGGGG
  TCCCTGCCTCCTTGTCAGATGTCCCAAAGACCACGGAGACCGGGAGACTGGAACAGCTCCGGCCCCAGGA
  GCTCTTGAGAGTCCAGGAGCCGGCTCCTCCCAGTGGTGAGAAGGCCAGGCTGAGCGAGGCCCCTGGTGGC
  AAGAAGAGCCTGAGCATGCTCCATTACCTCCGGGGCGCCGCGCCCAAGGACATTCCTGTGCCGTTGTCTC
  ACAGCATCAACGGGAAGAGCAAGCCCTGGGAGCCTTTCGTGGCCGAAGAGTTTGCACATCAATTCCACGA
  GTCCGTGCTGCAGTCCACACAGAAGGCTCTGCAGAAGCATAAAGGGAACTCTGCTTTGCTATCTGCAGAG
  CAGAGCCACAAAGTCGACACGGCAATCCACTACAACATTCCCGAGCTGCAGTCCTCCAGCAGGGTCCCCT
  TGCCCCAGCACAATGGACAACAGGAGCCTCCAATGGGGAGGAAGGGCCCTCCTATGCAGGAGGCGGACCA
  GGACTCTGAGGAGGACTCTGAGGAGGACAGCGAGGAGGAAGCTGAGGAAGCCCCGAGGCGCCAGTGGCAA
  GGGATTGAGGCAATCTTTGAAGCTTACCAGGAACACATAGAAGAGCAAAACCTGGAGCGGCAGGTGCTGC
  AGACGCAGTGCCGGCGGTTGGAGGCGCAGAACTACAGCCTCAGCCTGACTGCGGAGCAGCTGTCTCACAG
  CATGGCGGAGCTTCGGAGTCAGAAACAGAAGATGGTCTCTGAGCGGGAGCGGCTCCAGGCGGAGCTGGAC
  CACTTGCGGAAGTGCCTTGCCTTGCCCACCATGCACTGGCCTAGGGGCTACTTTAAGGGATATCCGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215292 representing NM_001145896
  Red=Cloning site Green=Tags(s)
  
  MKGMSHEPKSPSIGMLSTATRTTATVNPLTPSPLNGALVPTGSPATSSTLSAQAAPSSSFAAALRKLAKQ
  AEEPRGSSLSSESSPVSSPATNHSSPASTPKRVPMGPIIVPPGGHSVPSTPPVVTIAPTKTVNGVWRSES
  RQDSGSRGSSSGRERLLVEPPLAQEKAAGPAIPSHLLSTPYPFGLSPGSVVQDSRFQPLNLQRPVHHVVP
  PSTVTEDYLRSFRPYHTAEDLRMSSLPPLGLDPATAAAYYHPSYLAPHPFPHPAFRMDDSYCLSALRSPF
  YPIPTPGSLPPLHPSAMHLHLSGVRYPPELSHSSLAALHSERMSSLSAERLQMDEELRRERERERERERE
  ADREREKEREREQREKEREKELEREREKERERELERQREQRAREKELLAAKALEPTTFLPVAELHGLRGH
  STEERPKPSEQLTPTRAEKLKDVGLQAPKPVQHPLHPVPAPHHTVPSLISSHGIFSLPGSSATTALLIQR
  TNEEEKWLARQRRLRQEKEDRQSQVSEFRQQVLEQHLDLGRPLVPTEAEHRPESTRPGTNRHEQGSREPP
  QHFGGPPPLISPKPQQHTVPTALWNPVSLMDNALETRRAESHSLHSHPTAFEPSRQAAVPLVKVERVYCS
  EKAEEPRKREATPLDKYQPPPPPPPPREAGSLEPQTFPHGPGPFLTELEKSTQTILGQQRPSLSQATSFG
  ELSGPLKPGSPYCHPTARGPDPAYIYDEFLQQRRKLVSKLDLEERRRREAQEKGYYYDLDDSYDESDEEE
  VRAHLRCVAEQPPLKLDTSSEKLEFLQLFGLTTQQQKEELVAQKRRKRRRMLRERSPSPPAVQCKRQTPS
  PRLALSTRYSPDEMNNSPNFEEKRKFLTFFNLTHISAEKRKDKERLVEMLRAMKQRALSAADSVTNSSRD
  SPPVSLSEPATQPAPLETDQPVGVPASLSDVPKTTETGRLEQLRPQELLRVQEPAPPSGEKARLSEAPGG
  KKSLSMLHYLRGAAPKDIPVPLSHSINGKSKPWEPFVAEEFAHQFHESVLQSTQKALQKHKGNSALLSAE
  QSHKVDTAIHYNIPELQSSSRVPLPQHNGQQEPPMGRKGPPMQEADQDSEEDSEEDSEEEAEEAPRRQWQ
  GIEAIFEAYQEHIEEQNLERQVLQTQCRRLEAQNYSLSLTAEQLSHSMAELRSQKQKMVSERERLQAELD
  HLRKCLALPTMHWPRGYFKGYPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145896
• ORF Size: 3639 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145896.1, NP_001139368.1
• RefSeq Size: 6584 bp
• RefSeq ORF: 3642 bp
• Locus ID: 382034
• UniProt ID: Q3U3C9
• Cytogenetics: 8 E1
• MW: 136.7 kDa