Plekha5 (NM_144920) Mouse Tagged ORF Clone

CAT#: MR213825

Plekha5 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family A member 5 (Plekha5)

Product Images

Circular map for MR213825

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plekha5
• Synonyms: 2810431N21Rik; AI428202; AK129423; AMH-Cre; Ayu21-9; Gt(pU21)9Imeg; Pepp2; Tg(AMH-cre)1Flor
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR213825 representing NM_144920
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGACCTGAACCTGGAGTGGATCTGCTCGCTGCCGCGCTCCTGGACGTACGGGATCACTAGGG
  GCGGCCGCGTCTTCTTCATCAACGAGGAGGCCAAGAGCACCACCTGGCTACACCCGGTCACCGGCGAGGC
  CGTGGTCACCGGGCACCGGCGGCAGAGCACAGATTTGCCCACTGGCTGGGAAGAAGCATATACGTTCGAA
  GGTGCAAGATACTATATAAACCACAATGAAAGGAAAGTGACCTGCAAGCATCCGGTTACAGGACAGCCAT
  CCCAAGACAACTGTATCTTTGTTGTGAATGATCAGACTGTTGCAACCATGACGTCCGAAGACAAGAAGGA
  ACGACCCATAAGTATGATAAATGAAGCTTCCAATTACAACATGGCTTCAGATTATGCAGTGCATCCCATG
  AGCCCTGTGGGCAGGACCTCGCGGGCCTCCAAAAAGGTTCATAATTTTGGGAAAAGGTCCAATTCAATTA
  AAAGGAACCCTAATGCACCTGTGGTCCGGCGTGGCTGGCTGTATAAGCAGGACAGCACCGGCATGAAGCT
  GTGGAAGAAGCGCTGGTTTGTGCTCTCTGACCTTTGCCTTTTCTATTACCGAGATGAGAAGGAGGAGGGT
  ATTTTGGGAAGTATCCTGTTGCCTAGTTTTCAGATAGCCATGCTCACTGCTGAGGACCACATCAATCGCA
  AATATGCTTTTAAGGCAGCCCACCCAAACATGCGGACCTATTATTTCTGCACCGATACAGGAAAGGAGAT
  GGAGCTGTGGATGAAGGCCATGTTAGATGCTGCTCTGGTGCAGACAGAACCTGTGAAAAGAATTACCTTT
  AATTTCCGAGTGGACAAGATTACAACTGACAGTGCATCAACCAAAGAAACCAATAACATTCCCAACCACA
  GAGTTCTCATTAGACCCGAGGTCCAAAACCATCAGAAGAATAAGGAAATCAGCAAAATTGAAGAAAAAAG
  GGCCTTAGAAGCTGAAAGATACGGATTTCAGAAGGACGGGCAAGATAGACCATTAACAAAAATTAACAGT
  GTGAAGTTGAACTCCCTGCTATCAGAATATGAAAGCGGGCCAGACTGCCCACCTCAGAATGTACACTACA
  GACCCATCAATGTGAATAGTTCTGACGGCAAAGCGGTGAATGTCAGCTTGGCAGATGTCCGGGGTGGGAG
  TCACCCAAATGCAGGGCCCCTAGCCACAGAGGCCGACCGAGTCATTCAGAGAACGAATTCAATGCAACAG
  CTAGAGCAGTGGATTAAAGTCCAGAAAGGACGGGGTCTTGAAGAAGAGCCCAGAGGAGTAATTTCTTATC
  AAACATTGCCAAGAAATATGCCAAGCCACAGAGCCCAGATCCTGGCCCGCTGCCCTGAAGGTTACCGGAC
  ACTCCCGAGAAACAGCAAGACCAGACCTGAGAGTATCTGCAGCGTAACCTCTTCTGGTCATGAGAAGACA
  GGGCCGGGAGCAGAAGAGAAGCGGCGATCCATGAGAGATGACACCATGTGGCAGCTGTACGAGTGGCAGC
  AGCGCCAGTTTTACCACAAGCAGAGCACCCTCCCTCGGCACGGCTGCCTGAGCAGTCCCAAAGCCATGGT
  GCAGGTGTCTGACCAGACGATGCACTCCATCCCCACGTCACCTTCCCATGGGTCAGCAGCTGCTTACCAG
  GGCTTCTCCCCTCAGCGGACGTACAGGTCAGAAGTGACCTCGCCCATCCAGAGAGGAGATGTGACGATAG
  ACCGCAGGCACAGGCCTCATCACCCTAAGCATGTCTATGTAGCTGACAGAAGGTCAATGCCAGCTGGCTT
  AACTTTACAGGCTGTTAGTCCCCAGAGCCTCCAAGGCAGAACGCCAGAGGAGCTCACACTGCTGCTAATA
  AAGCTGAGACGGCAGCAGGCAGAGCTGAGTAGTGTCCGGGAGCACACCTTAGCACAGCTCATGCAGCTGA
  AACTTGAGGCCCACAGCCCAAAGAATGAGATTCTTTCACATCATCTCCAAAGGAACACCATATATTTGGA
  TCATCAGATGAAAGAAAATGAACCTATTATCACCATGGTTCACACAATGATTGAGAACTCGGCGCTAAGA
  CCACAACTGTACCAACAATTCTTAAGACAGAAGAACAAGATAAGTCTCTATTGTCTGTCACAAGATGAAT
  GTAGAGGCACATTATACAAATACAGACCTGAAGAAGCAGGTATTGATGCCAAATTAAGCAGATTGTGTGA
  ACAGGATAAAGTGGTCCGAGCGCTGGAGGAGAAGCTGCAGCAGCTGCACAAGGAGAAATACACGCTTGAG
  CAAGCCTTGCTGTCAGCCAGCCAAGAGATAGAGATGAATGCTGATAACCCAGCTGCCATTCAAACTGTGG
  TGTTGCAGAGGGATGACCTGCAAAACGGACTGCTGAGCACGTGTCGGGAACTCTCCCGAGCCACTGCTGA
  GCTGGAACGAGCCTGGAGAGAATATGACAAGTTGGAATATGATGTGACTGTTACCAGGGACCAGATGCAG
  GGTCAGCTCGACCGCCTTGGAGAAGTGCAGAGTGAGTCGGCAGGAATTCAACGGGCACAGATTCAAAAGG
  AGCTCTGGAGAATTCAAGATGTCATGGAGGGGTTGAGCAAACACAAACAGCAAAGAGGCTCTTCAGAAAC
  AGGTCTGGCAGGATCAAAGCCTTTCTCATCAGTTAAGTACAAGAGCGAGGAAGAGGAAGTAGTCCCACCT
  CGGCCTCCACTTCCTCGGTCTTATGACTTTACAGAGCAGCCTCCCATAATCCCCCCTCTGCCCAGTGATA
  GCAGCTCCTTGCTCTGTTATAGCAGGGGCCCCGTGCATCTGCCTGAAGACAAGAAGATTCATCAAGTTCA
  AGGATATCCAAGAAATGGATCTCACTGTGGTCCAGATTATAGACTCTACAAGAGTGAGCCAGAGTTAACC
  ACAGTGGCAGAAGTCGATGAATCTAACGGAGAAGAAAAGTCAGAGCCAGTTTCTGAGACAGAAGCTCCAG
  TCGTTAAAGGTTCCCATTTTCCAGTCGGAGTTCCTCTGAGAACAAAGTCACCCACACCCGAGTCCTCCAC
  GATAGCTTCCTATGTGACCTTGAGGAAGACTAAGAAGATGGTGGAGCTAAGAACGGAAAGACCAAGAAGT
  GCCGTGGAGCAGCTGTGTCTGGCTGAGAGTGCTCGACCTAGAATGACTGTGGAAGAGCAGCTAGAGAGGA
  TCAGGAGACACCAGCAGGCCTGCCTGAGGGAGAAGAAGAAGGGGCTGAGTGTCCTCGGTGCTTCCGACCC
  GTCAGACGTGAGGGACAGTCCACTCAGGCTTACTCAGACTCTAAGGAGGGATGATAACGTTAAGGAGCTA
  GACACCGTCCACAGAGAAAATGATGTAAAGCCAGACTACGAGACTCCAGCAGCACAGTGTGCTCACCTAG
  AAGACGCTGAACCCCAGAATGCAGACATCGGCAGAAAGCTAAAAAGATCTGAAATGCTTTACACACCTGA
  ACCAAATGGAATGGCTTCTGAGGAAGTGACGGAGAAGGAAAGACAGAAAGAGCAGGTGCATGCCGATGGG
  TCATGCAGCCCTCAGGAAGAGACGGCCATGACAGAGCATCAGATGGAAGGCCCCCCTGAGGAAGCAGAGA
  GTCTTCATGAGGAGGAAGAAACTCTTGCTTCCTGTGAGCCAGCTCCTGAGATTCCTAGGGAGAATCAAAC
  CACAGTGAGAAGTCTGTCCCCGTCTCCTGACTCCTCCACGGCAGCAGATCCGCCCACTCCTCCACAGCTC
  AGGGAAGGATCGCATTTCATGTGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR213825 representing NM_144920
  Red=Cloning site Green=Tags(s)
  
  MAADLNLEWICSLPRSWTYGITRGGRVFFINEEAKSTTWLHPVTGEAVVTGHRRQSTDLPTGWEEAYTFE
  GARYYINHNERKVTCKHPVTGQPSQDNCIFVVNDQTVATMTSEDKKERPISMINEASNYNMASDYAVHPM
  SPVGRTSRASKKVHNFGKRSNSIKRNPNAPVVRRGWLYKQDSTGMKLWKKRWFVLSDLCLFYYRDEKEEG
  ILGSILLPSFQIAMLTAEDHINRKYAFKAAHPNMRTYYFCTDTGKEMELWMKAMLDAALVQTEPVKRITF
  NFRVDKITTDSASTKETNNIPNHRVLIRPEVQNHQKNKEISKIEEKRALEAERYGFQKDGQDRPLTKINS
  VKLNSLLSEYESGPDCPPQNVHYRPINVNSSDGKAVNVSLADVRGGSHPNAGPLATEADRVIQRTNSMQQ
  LEQWIKVQKGRGLEEEPRGVISYQTLPRNMPSHRAQILARCPEGYRTLPRNSKTRPESICSVTSSGHEKT
  GPGAEEKRRSMRDDTMWQLYEWQQRQFYHKQSTLPRHGCLSSPKAMVQVSDQTMHSIPTSPSHGSAAAYQ
  GFSPQRTYRSEVTSPIQRGDVTIDRRHRPHHPKHVYVADRRSMPAGLTLQAVSPQSLQGRTPEELTLLLI
  KLRRQQAELSSVREHTLAQLMQLKLEAHSPKNEILSHHLQRNTIYLDHQMKENEPIITMVHTMIENSALR
  PQLYQQFLRQKNKISLYCLSQDECRGTLYKYRPEEAGIDAKLSRLCEQDKVVRALEEKLQQLHKEKYTLE
  QALLSASQEIEMNADNPAAIQTVVLQRDDLQNGLLSTCRELSRATAELERAWREYDKLEYDVTVTRDQMQ
  GQLDRLGEVQSESAGIQRAQIQKELWRIQDVMEGLSKHKQQRGSSETGLAGSKPFSSVKYKSEEEEVVPP
  RPPLPRSYDFTEQPPIIPPLPSDSSSLLCYSRGPVHLPEDKKIHQVQGYPRNGSHCGPDYRLYKSEPELT
  TVAEVDESNGEEKSEPVSETEAPVVKGSHFPVGVPLRTKSPTPESSTIASYVTLRKTKKMVELRTERPRS
  AVEQLCLAESARPRMTVEEQLERIRRHQQACLREKKKGLSVLGASDPSDVRDSPLRLTQTLRRDDNVKEL
  DTVHRENDVKPDYETPAAQCAHLEDAEPQNADIGRKLKRSEMLYTPEPNGMASEEVTEKERQKEQVHADG
  SCSPQEETAMTEHQMEGPPEEAESLHEEEETLASCEPAPEIPRENQTTVRSLSPSPDSSTAADPPTPPQL
  REGSHFMCV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144920
• ORF Size: 3807 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144920.3, NP_659169.3
• RefSeq Size: 5133 bp
• RefSeq ORF: 3810 bp
• Locus ID: 109135
• Cytogenetics: 6 G2
• MW: 144.4 kDa