Stpg2 (NM_198659) Mouse Tagged ORF Clone

CAT#: MR213559

Stpg2 (Myc-DDK-tagged) - Mouse RIKEN cDNA B930007M17 gene (B930007M17Rik)

Product Images

Circular map for MR213559

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stpg2
• Synonyms: B930007M17Rik; Gm1017
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR213559 representing NM_198659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGACCGTGCTCCGCGCTGGCTCGACTGTGCCAACCGCGGCAGCACCGAGGAGCACGTGGGGCCTG
  GGACCTACCAGGTACCTTTTCCTAAGCAGCAGGCCACAGGTTGCTATGCACCATTTCTTTCTTTGAGTTC
  CAAAACAAGTGCTTGTGTTGTCTCCTCTGATGCTGGCCAAGCTGTTCCAGGTCCAGCACACTACAACGTA
  TCACAAGCACAGTATAATATAAGAGGAGGCCGCAGTCTGCAAAACCGAGAGAAGCGATTTAAGAAACTTA
  TTTCGGATGGTCCAGGACCTGGGAGCTACAATTGGCCTTATCTTGGGACATTATGTATCACAACCAGACA
  GAAAACTCCTAGAACTCCTGCAGTTTCCAGAAACATTGATATTCCTTCAATCCCCTCTTCCGGAAAGTCA
  CATGGGTATCATCTCAATGACGATGACACTATTATGAGACGCACCCCACCTCCTAGTGACAACACAATAG
  GTCCAGCGTACTACAATCCTCAGTTTGATTATCCTAAAGCATCTTTGAAATATAAAGGCGTAAATTTTGG
  CAATGCTACAGGAAGACAAGAATTTCTCAAGTATTCGGGTCCTGGTCCTGGACAGTATGATATCATCCAG
  AAAAGGAAACTACATTGTGAAAATATTAACATCAAGAGAGAACAAGAGCATAATTATTATACATATGTCC
  CACGGCTATATGAAGCGATAATATTGCAGGAGGAAAAAAAGGGTGTACCAGGACCTGGAAAGTACAACAT
  TAAAAGTGAATTTGACATGATAAAATCCATGTCAGCACTTGTGAATTCTCCATCTTTTATTTTCTTTTCT
  GAAACAGAGAGATTTGAACCTATAAAATCATGCACCCCAGCTCCTGGCACATACAATGAAATACGAACTG
  CTTTCAAGTGCCCAAAGAAAAGATTTGGACTGTCTTTGCCATTTAACCAGAGTGCTGCTCGATTCACAGA
  AGATTCCAAGGCACAGAAGCTACCAGGTCCTGGCTTTTATGATATCTCAACCAACATTGTAAAAGCCCAG
  GTTAAAAAACCCTGCTTGAAGAAACAACCGAAAACTGGATTTGGTTCTTCTGTGCCTCGAACTTTGTTCA
  CAGCTCAGAAAAAAGCTTTCCGTGGTCCTGGGCCTAGTGATTATCAGGTTCGAGGAACTCATGATGAATT
  ACCTAACTTGAACAAATCTGCTGCTTTCTTGTCGAGAGCAGAAAAAACTCCACCAGTACGAAAAATGCGT
  CTTCCAGCTCCTGGCCGCTATGATGTTCAGAAATCGTATGACATGTCCCAAGTTAAACATAAGTACATGC
  CACCTCGTACTTCGGTGGCTAAAAAAAGGCATTCCTCTTTCCTGAGCGCAGCTCCTCGGTGCCTGGGAAA
  AATCGCTGATGGGCCAGGACCTGCAACATACAGTCCTGTTTTAATGAAGTCTGGTGCCATAATCTCATTC
  GTTAAAGGACCAAAGCGTTTTCAAGAGTTTCATGGCGAGTTTTCTCCAGGCCCTACGACATATGAGCTCA
  GTCCATTTCTAAGACATTCTCTTCTGAAGAGAACATACAATGTCACTCTGCCGTGTTCATCCTCTCCTAA
  TAGAGAAAATACTGGTTGTCCATCACAAAAAGCCACACAAAAATTTCAAAGAGAGAAATTACAGTATTTT
  AAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR213559 representing NM_198659
  Red=Cloning site Green=Tags(s)
  
  MYDRAPRWLDCANRGSTEEHVGPGTYQVPFPKQQATGCYAPFLSLSSKTSACVVSSDAGQAVPGPAHYNV
  SQAQYNIRGGRSLQNREKRFKKLISDGPGPGSYNWPYLGTLCITTRQKTPRTPAVSRNIDIPSIPSSGKS
  HGYHLNDDDTIMRRTPPPSDNTIGPAYYNPQFDYPKASLKYKGVNFGNATGRQEFLKYSGPGPGQYDIIQ
  KRKLHCENINIKREQEHNYYTYVPRLYEAIILQEEKKGVPGPGKYNIKSEFDMIKSMSALVNSPSFIFFS
  ETERFEPIKSCTPAPGTYNEIRTAFKCPKKRFGLSLPFNQSAARFTEDSKAQKLPGPGFYDISTNIVKAQ
  VKKPCLKKQPKTGFGSSVPRTLFTAQKKAFRGPGPSDYQVRGTHDELPNLNKSAAFLSRAEKTPPVRKMR
  LPAPGRYDVQKSYDMSQVKHKYMPPRTSVAKKRHSSFLSAAPRCLGKIADGPGPATYSPVLMKSGAIISF
  VKGPKRFQEFHGEFSPGPTTYELSPFLRHSLLKRTYNVTLPCSSSPNRENTGCPSQKATQKFQREKLQYF
  N
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198659
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198659.2, NP_941061.1
• RefSeq Size: 2818 bp
• RefSeq ORF: 1686 bp
• Locus ID: 381476
• UniProt ID: Q8C8J0
• Cytogenetics: 3 H1
• MW: 62.7 kDa