Gpr137c (NM_027518) Mouse Tagged ORF Clone

CAT#: MR213244

Gpr137c (Myc-DDK-tagged) - Mouse G protein-coupled receptor 137C (Gpr137c)

Product Images

Circular map for MR213244

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpr137c
• Synonyms: 6330416L11Rik; Gm908; TM7SF1L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR213244 representing NM_027518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTATCAGTGTCCGGGCCGGCGGCCGCCGCGGTCCCCACGGCCGGCCGCGAACCCTCGACGTCCG
  GCGGGGGCGCCGTCGCGGCCTCGGGCGCCGCGGTTCCCGGTTCGGTGCAGCTGGCGCTGAGCGTGCTGCA
  CGCGCTGCTCTACGCCGCGCTCTTCGCCTTTGCCTACCTGCAGCTGTGGCGGCTGCTCCTGTACCGCGAG
  CGGCGGCTCAGTTACCAGAGCCTCTGCCTCTTCCTGTGTCTCGCGTGGGCAGCGCTCAGGACCACGCTCT
  TCTCGGCCGCCTTCTCCCTCAGCGGCTCTCTGCCCCTGCTGCGGCCACCCTCTCGCCTGCACTTCTTCCC
  GCACTGGCTGCTCTACTGCTTCCCCTCCTGCCTACAGTTCTCCACGCTCTGTCTCCTCAACCTCTACCTG
  GCAGAGGTGATATGTAAAGTCAGATGTGCCACTGAACTTGACAAACACAAAATTCTACTGCATTTGGGCT
  TTATACTGGCAAGCCTCCTCTTCTTAGCAGTGAACTTGACCTGTGCAATGCTAGTCCACGGAGATGTCCC
  AGAAAATCAATTGAAATGGACTGTGTTTATTCGAGCACTAATTAACGATAGCCTGTTTATTCTCTGTGCC
  ATCTCTTTAGTTAGCTACATATGCAAAATTACAAAAATGTCATCAGCAAATGTCTACCTCGAATCAAAGG
  GTATGTCTCTGTGCCAGACTGTCATCGTGGGCTCTGTAGTCATTCTTCTCTACTCTTCCAGAGCTTGTTA
  TAATCTGGTAGTGATCACCATATCACAGGATACCTTAGAGAGTCCATTTAATTATGGCTGGGATAATCTT
  TCAGATAAGGCTCAGGGAGAAGACGGAAATGGAGGAGACTACATTGTCTTTGGAATGGTCCTCTTTCTGT
  GGGAGCATGTACCAGCATGGTCAGTGGTACTATTTTTCCGGGCACAGAGATTAAACCAGAATCTGGCACC
  TGCTGGCATGATAAGCAGCCACAGCTACAGCTCCAGGGCTTTCTTTTTCGACAATCCAAGACGGTATGAC
  AGTGACGACGACTTGCCAAGACTGGGGAACTCACGGGAAGGAAGTTTATCAAACTCGCAAACTTTGGGCT
  GGTACGGCACCATGACTGGGTGTGGCGGCGGCACCTACACAGTCACACCCCCACTGAATGGACCTGTGAC
  AGACACTGCTCCTTTGCTCTTCACGTGCAGTAATTTAGATATAAGGAATCACCATAGCTTATACGTAACT
  CCACAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR213244 representing NM_027518
  Red=Cloning site Green=Tags(s)
  
  MRVSVSGPAAAAVPTAGREPSTSGGGAVAASGAAVPGSVQLALSVLHALLYAALFAFAYLQLWRLLLYRE
  RRLSYQSLCLFLCLAWAALRTTLFSAAFSLSGSLPLLRPPSRLHFFPHWLLYCFPSCLQFSTLCLLNLYL
  AEVICKVRCATELDKHKILLHLGFILASLLFLAVNLTCAMLVHGDVPENQLKWTVFIRALINDSLFILCA
  ISLVSYICKITKMSSANVYLESKGMSLCQTVIVGSVVILLYSSRACYNLVVITISQDTLESPFNYGWDNL
  SDKAQGEDGNGGDYIVFGMVLFLWEHVPAWSVVLFFRAQRLNQNLAPAGMISSHSYSSRAFFFDNPRRYD
  SDDDLPRLGNSREGSLSNSQTLGWYGTMTGCGGGTYTVTPPLNGPVTDTAPLLFTCSNLDIRNHHSLYVT
  PQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027518
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027518.2, NP_081794.2
• RefSeq Size: 2444 bp
• RefSeq ORF: 1272 bp
• Locus ID: 70713
• UniProt ID: E9Q343
• Cytogenetics: 14 C1
• MW: 46.9 kDa
• Gene Summary: Lysosomal integral membrane protein that may regulate MTORC1 complex translocation to lysosomes.[UniProtKB/Swiss-Prot Function]