Helz (NM_198298) Mouse Tagged ORF Clone

CAT#: MR212103

Helz (Myc-DDK-tagged) - Mouse helicase with zinc finger domain (Helz)

Product Images

Circular map for MR212103

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Helz
• Synonyms: 3110078M01Rik; 9430093I07Rik; 9630002H22Rik; AI851979; DRHC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212103 representing NM_198298
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACAGAAGAGCTGAAAGGTCATGTGAACAAGCGTGCGCGTCACTGCAGAGGCAGGACTATGACA
  TGGCCCTCCAGCACTGCACCGACGCCCTGCTCTCTCTTGGCCAGTACTCCATGGCAGACTTCACAGGGCC
  GTGCCCGGTGGAGGTTGAGCGCATCAAAATTGAGAGTCTTCTTTACAGAATTGCCTCATTCTTGCAATTG
  AAAAACTACGGGCAAGCTGATGAAGATTGTAGGCATGTGCTGGGGGAAGGACTGGCCAAGGGAGAGCGTG
  CCTTCCGGGCAGTGCTGTGCTGCATGCAGCTGAAAGGGAAGCTCCAGCTTGTGTCCTCCATCCTTGCCAA
  GTCACTGTCAGGGGAGTCTCTGAATGGGATGGTAACGAAAGATTTGACAAGACTAAAGACACTTCTCACA
  GAAACAGAGACAGCAACGAGTAATGTCCTGTCTGGATGTCACGTAGAAGACTTAGATGAAGGGTCCTGTA
  ATGGTTGGCATTTCCGCCCACCGCCCAGGGGCATCACCAGCAGTGAGGAGTACACCTTGTGTAAGAGGTT
  TTTAGAGCAAGGGATCTGTAGGTACGGTGCCCAGTGTACTTCAGCACATTCCCAGGAAGAGCTAGCAGAG
  TGGCAGAAAAGATACGCCTCCCGACTGATCAAACTGAAGCAGCAAAGTGAGAATAAACAGCTCTCAGGCA
  GCTACATGGAGACCTTGATAGAGAAGTGGATGAGTTCACTGTCTCCTGAGAAAGTGCTTAGTGAATGCAT
  AGAGGGAGTTCAGGTGGAGCACAGCCCTGACCTGTCGGTCACCGTCAACACCAAAAAATCCCACCAGACG
  TGGACCTTTGCTCTGACCTGTAAGCCTGCAAGAATGCTGTATCGGGTAGCCTTGCTTTATGACGCTCATC
  GTCCTCATTTCAGTATCATTGCCATATCTGCCGGAGATAGTACTACCCAGGTATCACAAGAAGTCCCCGA
  GAACTGTCAAGAATGGATAGGAGGAAAGATGGCCCAAAATGGATTAGATCATTACGTGTATAAAGTCGGG
  ATAGCATTTAACACAGAAATATTTGGAACTTTTCGCCAAACCATAGTTTTCGACTTTGGATTGGAGCCAG
  TACTCATGCAGAGGGTGATGATTGACGCAGCTTCTACAGAAGATCTTGAATACCTGATGCATGCAAAGCG
  GCAGCTGGTAACCACAGCCAAGCGCTGGGACTCTTCTTCTAAGACTATTGTAGATTTTGAACCTAATGAA
  ACTACTGATTTGGAGAAGAGCCTTTTGATCAGATACCAAATTCCTCTGTCTGCTGACCAGCTGTTTACAC
  AGTCCGTCTTAGACAAATCATTGACCAAGACTAACTATCAGGCACGGCTGCACGACCTGCTTTATATTGA
  GGAGATAGCCCAGTATAAAGAAGTCAGCAGGTTCAACCTTAAAGTGCAATTGCAGATTTTAGCAAGCTTC
  ATGCTCACTGGAGTTTCTGGGGGTGCAAAGTATGCTCAGAATGGACAACTTTTTGGTCGTTTCAAGCTTA
  CTGAAACCCTCTCGGAAGACACTTTGGCTGGACGGCTGGTGATGACCAGGGTCAATGCTGTTTATTTATT
  ACCAGTCCCTAAAGAGAAGCTCGTTCAGTCCCAGGGAACCAAAGAGAAGGTTTATGAAGCTACTATTGAA
  GAAAAAACAAAAGACTATGTATTTTTAAGGATATCCAGGGAATGCTGTGAAGAGCTTAGTCTGCGGCCTG
  ACTGTGACATCCAGGTTGAGCTTCAGTTTCAGTTGAACCGATTACCCCTCTGTGAAATGCATTATGCACT
  AGACAGGATTAAGGACAATGCTGTTTTGTTTCCAGACATCAGTATGACTCCTACCATACCTTGGAGTCCC
  AACAGACAGTGGGACGAGCAGTTGGATCCTCGGCTAAATGCAAAGCAGAAAGAAGCTGTTCTGGCCATCA
  CCACTCCACTCTCCATCCAGCTGCCGCCTGTCCTCATCATTGGACCCTACGGGACGGGCAAAACCTTCAC
  TCTGGCTCAGGCGGCCAAGCACATACTGCAGCAGCAGGAGACCAGCAGGATTCTCATTTGCACCCATTCT
  AATAGTGCTGCTGACCTCTACATAAAGGATTATTTACATCCATATGTAGAAGCAGGCAATCCCCAGGCAA
  GACCTCTCAGGGTGTATTTCCGGAATCGCTGGGTGAAGACTGTCCACCCAGTCGTGCATCAGTACTGCTT
  GATCTCGAGCACACAGTCCACCTTTCAGATGCCCCAGAAGGAAGATATTCTTAAACATCGAGTGGTGGTG
  GTCACACTGAGCACTTCCCAGTACCTCTGCCAGCTGGACCTGGAACCTGGGTTCTTCACGCACGTTCTCT
  TAGATGAAGCTGCCCAGGCCATGGAGTGTGAGACCATTATGCCTCTAGCCTTAGCTACTAAAAACACACG
  GATTGTTTTGGCTGGTGATCACATGCAGCTCAGCCCTTTTGTTTACAGCGAGTTTGCCAGGGAGAGAAAC
  CTTCACGTTTCGTTACTTGACCGACTCTACGAGCATTACCCTGCTGAGTTTCCATGCAGGATCCTCCTCT
  GTGAGAACTATCGTTCCCATGAAGCTATCATCAACTACACCTCTGAGCTTTTCTATGAGGGGAAGCTGAT
  GGCCAGTGGGAAGCAGCCGGCGCACAAGGACTTCTATCCACTCACGTTCTTTACGGCACGGGGGGAGGAC
  GTCCAAGAGAAGAACAGCACGGCATTCTACAACAATGCAGAGGTATTTGAAGTGGTGGAGCGTGTAGAAG
  AGCTAAGACGGAAGTGGCCAGTGGCATGGGGAAAGTTAGATGACGGCAGCATCGGTGTGGTGACGCCATA
  CGCTGACCAGGTGTTTAGAATCCGAGCTGAGCTTCGGAAGAAGAGGCTCTCGGATGTGAATGTGGAAAGG
  GTGCTCAATGTCCAAGGAAAGCAGTTCAGAGTTCTGTTTCTTAGCACAGTGCGTACACGCCACACTTGCA
  AGCACAAGCAGACGCCAATTAAAAAGAAAGAGCAACTCCTGGAAGACTCCACCGAGGACTTAGATTATGG
  TTTTCTATCCAACTACAAACTTCTCAATACGGCCATCACCAGAGCACAGTCCTTGGTTGCTGTGGTGGGT
  GACCCTGTTGCTCTGTGCTCTATTGGAAGGTGTAGAAAGTTTTGGGAGCGGTTTATTGCCCTGTGTCATG
  AAAACCACAGCTTGCACGGGATCACGTTCGAACAGATCAAGGCACAGTTAGAGGCTTTAGAACTCAAGAA
  GACTTACGTGTTGAATCCACTGGCTCCTGAGTTTATCCCCCGGGCTTTACGACTGCAGCATTCAGGAAAC
  AGTAGCAGACAGCAGCAGTCACCACCCAAGGTGAAAAGCCTCTATCATCCCCAGAGTGATCATTTCCAGA
  GTGATGGAATTGTTCAGCCCAATCCTTCCGTACTTATTGGCAATCCTATTAGAGCATATACTCCTCCGCC
  CCCTCTTGGACCGCACCCAAACTTGGGAAAATCTCCAAGCCCCGTTCAAAGAATAGATCCTCACACTGGG
  ACTAGTATTCTTTACGTACCTGCTGTCTATGGAGGAAATGTAGTTATGTCGGTGCCTTTACCTGTGCCAT
  GGACAGGGTACCAGGGTAGGTTTGCAGTTGACCCTCGGATTATCACACACCAGGCAGCAATGGCCTACAA
  TATGAACCTATTACACACACACGGACGTGGGTCACCGATCCCTTATGGTCTTGGCCATCACCCACCTGTC
  AGCCTAGGCCAGCCACAGAGTCAGCACGCAGAGAAGGATCAGCAGGAGCAAAACCGAAATGGTAAAACTG
  ATACAAATAACCCTGGACCTGAAATTAATAAGATCCGAACACCAGAGAAGAAACCTACAGAGCCCAAGCA
  GGTTGATTTGGAATCAAATCCACAGAACAGAAGTCCTGAATCCCGTCCTGGTGTTGTTTATTCCAACACT
  AAATTTCCTCGAAAAGATCATCTCAACCCAAGACACATAAATAATCTCCCTCTTCCTGCACCACACGCCC
  AGTATGCAATCCCTAGCCGCCATTTTCACCCGCTGCCTCAGCTGCCCAGGCCGCCGTTTCCCGCTTCCCA
  GCCGCACACCTTGTTAAATCAGCAACAGAATAATTTGCCTGAACAACCAAATCAGATGGCACCTCAGCCA
  AATCAGGTGGCACCCCAGCCAAATCAGATGACACCCCAGCCAAATCAGGTGGCACCCCAGCCAAATCAGG
  TGGTCCAGCAGCAGAGCCAGGCACCTCCACAGGCCCCACAGCCAGCACCTCAACTCTCTCCTGCATTTCA
  GGCGGGGCCCACCAATGCATTTTTCAATAATGCAGTTGCTCACCGACCACAGTCTCCTGCTGCAGAGGCT
  GTGGGTCCTGAGCAGCCGCCCCCTCCCGGGCTGCCCGATGGCCACAGTCCACTGAGAGCCATCACACAGC
  CTGGCCCCATCCTGGCATCACCTTTGAACAACTTTGTTGATGAGAGCTCTCCAGGATTGCCTATAGAAGA
  AGCTTTGGATGGAGTGCATGGAAGCGTAGCTCTGGAGACGCTGAGGCAGCAGCAGGCGCGGCTACAGCAG
  TGGAGTGAGCACCATGCCTACCTGAGCCAGGGCGGCATCCCCTATTCACACCATCACCATCCTCACCTCC
  CGCATCTTCCCCACACACCCATTGGCCTCCACCAGCCACCAGTGAGGGCAGAGTGGAAAGTCGCCGGCAG
  GGCTGACGATGAAACAGAGACAACATTCTCAAGGTTTCAAGACTTACTCAGAGAGCTGTCCCATCGGGAT
  CAAGGTGACACTGGAGAACTAGCTGAAATGCCACCACCTCAGTCAAGACTTCTGCAGTACAGACAAGTTC
  AGCCTAGAAGTCCGCCAGCGGTCCCGTCTCCCCCTTCCTCCACAGACCACAGTAGCCAGTTTGCTAACTT
  TAATGACAGCAGCAGAGACATTGAAGTAGCCAACAGCCCAGCATTCCCGCAGCGCCTCCCGCCTCAGCTA
  TTTGGCTCCCCTTTCTCGTTGCCATCTGAACACCTTGCCCCTCCTCCTCTGAAATACCTGGCTCCGGAAG
  GAGCATGGAATTTTGCTAACTTGCAGCAGAATCACTTAATAGGGCCGGGCTTTCCCTATGGCCTACCTCC
  ATTGCCTCCCAGGCCACCACAGAACCCTTTTATACACATCCAGAACCATCAACACGCTGCTGGTCAAGAG
  CCATTTCACCCACTGTCATCTCGAACAGTGTCTGCTTCTTCGCTCCCCAGCCTAGAAGAGTATGAGCCCA
  GAGGACCTGGTCGGCCCTTGTACCAAAGAAGAATCTCATCTAGCTCAGCCCAGCCTTGTGTGGAAGAGGC
  AAGCGCTCCCCAAGACAGCCTGGCTCAGGGCAAAGAATCGCAGGGCCACAGCAACCCACCTGCCTTCAAC
  TTCCCGGCCCCCGAGTCCTGGGCCAACACCACCTCATCTGCCCCCTACCAGAACATTCCGTGCAATGGAT
  CCAGCAGGACGAGTCAGCCCAGAGAGTTGATAGCTCCACCCAAGACTGTCAAACCCCCTGAGGATCAGCT
  GAAGCCCGAGAGCGGGGAGGTGTCCAGTTCCTTCAACTACTCGATGCTGCAGCACCTTGGCCAGTTCCCA
  CCCCTCATGCCCAACAAGCAGATCGCAGAGTCCGCCAACTGCAGCAGCCAGCAGAGCCCGGCAGGGAGCA
  AGCCCGCCATGTCCTACGCCAGTGCGCTGCGGGCCCCGCCCAAGCCCAGGCCGCCACCCGAGCAGGCCAA
  GAAGGGCAGCGACCCGCTGTCCCTGCTCCAGGAGCTCAGTCTGGGCAGCAGCCCGGGCAGCAATGGCTTC
  TACTCCTACTTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212103 representing NM_198298
  Red=Cloning site Green=Tags(s)
  
  MEDRRAERSCEQACASLQRQDYDMALQHCTDALLSLGQYSMADFTGPCPVEVERIKIESLLYRIASFLQL
  KNYGQADEDCRHVLGEGLAKGERAFRAVLCCMQLKGKLQLVSSILAKSLSGESLNGMVTKDLTRLKTLLT
  ETETATSNVLSGCHVEDLDEGSCNGWHFRPPPRGITSSEEYTLCKRFLEQGICRYGAQCTSAHSQEELAE
  WQKRYASRLIKLKQQSENKQLSGSYMETLIEKWMSSLSPEKVLSECIEGVQVEHSPDLSVTVNTKKSHQT
  WTFALTCKPARMLYRVALLYDAHRPHFSIIAISAGDSTTQVSQEVPENCQEWIGGKMAQNGLDHYVYKVG
  IAFNTEIFGTFRQTIVFDFGLEPVLMQRVMIDAASTEDLEYLMHAKRQLVTTAKRWDSSSKTIVDFEPNE
  TTDLEKSLLIRYQIPLSADQLFTQSVLDKSLTKTNYQARLHDLLYIEEIAQYKEVSRFNLKVQLQILASF
  MLTGVSGGAKYAQNGQLFGRFKLTETLSEDTLAGRLVMTRVNAVYLLPVPKEKLVQSQGTKEKVYEATIE
  EKTKDYVFLRISRECCEELSLRPDCDIQVELQFQLNRLPLCEMHYALDRIKDNAVLFPDISMTPTIPWSP
  NRQWDEQLDPRLNAKQKEAVLAITTPLSIQLPPVLIIGPYGTGKTFTLAQAAKHILQQQETSRILICTHS
  NSAADLYIKDYLHPYVEAGNPQARPLRVYFRNRWVKTVHPVVHQYCLISSTQSTFQMPQKEDILKHRVVV
  VTLSTSQYLCQLDLEPGFFTHVLLDEAAQAMECETIMPLALATKNTRIVLAGDHMQLSPFVYSEFARERN
  LHVSLLDRLYEHYPAEFPCRILLCENYRSHEAIINYTSELFYEGKLMASGKQPAHKDFYPLTFFTARGED
  VQEKNSTAFYNNAEVFEVVERVEELRRKWPVAWGKLDDGSIGVVTPYADQVFRIRAELRKKRLSDVNVER
  VLNVQGKQFRVLFLSTVRTRHTCKHKQTPIKKKEQLLEDSTEDLDYGFLSNYKLLNTAITRAQSLVAVVG
  DPVALCSIGRCRKFWERFIALCHENHSLHGITFEQIKAQLEALELKKTYVLNPLAPEFIPRALRLQHSGN
  SSRQQQSPPKVKSLYHPQSDHFQSDGIVQPNPSVLIGNPIRAYTPPPPLGPHPNLGKSPSPVQRIDPHTG
  TSILYVPAVYGGNVVMSVPLPVPWTGYQGRFAVDPRIITHQAAMAYNMNLLHTHGRGSPIPYGLGHHPPV
  SLGQPQSQHAEKDQQEQNRNGKTDTNNPGPEINKIRTPEKKPTEPKQVDLESNPQNRSPESRPGVVYSNT
  KFPRKDHLNPRHINNLPLPAPHAQYAIPSRHFHPLPQLPRPPFPASQPHTLLNQQQNNLPEQPNQMAPQP
  NQVAPQPNQMTPQPNQVAPQPNQVVQQQSQAPPQAPQPAPQLSPAFQAGPTNAFFNNAVAHRPQSPAAEA
  VGPEQPPPPGLPDGHSPLRAITQPGPILASPLNNFVDESSPGLPIEEALDGVHGSVALETLRQQQARLQQ
  WSEHHAYLSQGGIPYSHHHHPHLPHLPHTPIGLHQPPVRAEWKVAGRADDETETTFSRFQDLLRELSHRD
  QGDTGELAEMPPPQSRLLQYRQVQPRSPPAVPSPPSSTDHSSQFANFNDSSRDIEVANSPAFPQRLPPQL
  FGSPFSLPSEHLAPPPLKYLAPEGAWNFANLQQNHLIGPGFPYGLPPLPPRPPQNPFIHIQNHQHAAGQE
  PFHPLSSRTVSASSLPSLEEYEPRGPGRPLYQRRISSSSAQPCVEEASAPQDSLAQGKESQGHSNPPAFN
  FPAPESWANTTSSAPYQNIPCNGSSRTSQPRELIAPPKTVKPPEDQLKPESGEVSSSFNYSMLQHLGQFP
  PLMPNKQIAESANCSSQQSPAGSKPAMSYASALRAPPKPRPPPEQAKKGSDPLSLLQELSLGSSPGSNGF
  YSYFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198298
• ORF Size: 5895 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198298.2
• RefSeq Size: 6240 bp
• RefSeq ORF: 5898 bp
• Locus ID: 78455
• UniProt ID: Q6DFV5
• Cytogenetics: 11 E1
• MW: 220 kDa
• Gene Summary: May act as a helicase that plays a role in RNA metabolism in multiple tissues and organs within the developing embryo.[UniProtKB/Swiss-Prot Function]