Emsy (NM_172280) Mouse Tagged ORF Clone
CAT#: MR211868
- TrueORF®
2210018M11Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2210018M11 gene (2210018M11Rik)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_172280" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Emsy |
Synonyms | 2210018M11Rik; 2310016L09Rik; AU019521; AU019557 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211868 representing NM_172280
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGTTGTGTGGCCAACCCTTTTGGATCTCAGCAGGGATGAATGCAAACGGATTCTTCGAAAATTGG AACTGGAGGCATATGCTGGAGTTATCAGTGCACTTCGGGCACAAGGGGACCTCACCAAGGAAAAGAAAGA TCTTCTTGGAGAGCTCTCAAAAGTCCTTAGCATCTCAACAGAGCGCCACCGTGCTGAAGTTCGGAGAGCA GTAAACGATGAACGGTTAACAACAATTGCACATAAAATGAATTTATCCTTATATTTGGGTGAAAGACCAA GTTACAGTATGTCTGGACCTAATAGTTCTTCAGAGTGGTCCATCGAAGGACGTAGATTGGTACCTCTGAT GCCCAGGCTGGTTCCCCAGACTGCCTTCACTGTAACAGCGAATGCTGTGGCCAATGCAGCTGTCCAGCAC AATGCGTCCCTTCCTGTGCCTGCAGAGACAGCAAGCAAAGACGGCGTTAGCTGCTCAGATGAAGATGAAA AACCCAGAAAACGCAGGCGAACAAACTCCTCTAGCTCCTCCCCTGTTGTCCTCAAGGAAGTTCCAAAGGC TGTTGTTCCGGTCTCAAAGACGATCACTGTGCCTGTGAGTGGAAGTCCCAAGATGAGCAACATTATGCAG AGCATTGCCAACTCCTTACCACCCCATATGTCACCTGTGAAAATAACGTTTACCAAACCATCAACACAGA CAACAAACACAACAACACAGAAGGTTATTATAGTGACCACATCACCAAGTTCAACCTTTGTGCCCAACAT TCTCTCCAAATCTCATAACTATGCAGCAGTCACTAAGCTTGTACCAACATCAGTCATTGCCTCCACGACT CAGAAGCCACCTGTTGTTATAACTGCGTCCCAGGCCTCTCTGGTCACCAGTAGCAGCAATGGCAACAGCA GTTCTACGTCATCACCTATTTCTAGTACAGTTGCAGTAACAACGGTGGTATCTTCCACACCGTCCGTGGT CATGTCAACAGTAGCACAAGGTGTATCCACATCAGCAATCAAAGTGGCATCGACAAGACTTCCTTCCCCC AAAAGCTTAGTGAGTGGCCCTACCCAGATTCTTGCACAGTTTCCTAAACAGCATCAGCAGTCTCCTAAGC AGCAGTTACAGCAAGTACAACAACAGACACAGCAGCCAGTGGCCCAGCCTTCTTCAGTGTCTCAGCAGCA GCAGCCTCAGCAGTCAGCATTGCCGCCTGGTATCAAACCTACCATCCAGATCAAACAGGAGTCAGGTGTT AAAATCATCACACAGCAGGTTCAACCAAGTAAAATCTTACCCAAACCAGTGACTGCAACTCTACCCACCA GTAGCAATTCCCCAATTATGGTGGTTAGCAGTAATGGTGCAATTATGACAACTAAACTGGTAACCACTCC TACCGGTACTCAGGCAACCTATACCCGGCCAACAGTGAGCCCATCCTTAGGTCGTGTGGCTACAACCCCT GGAGCTGCAACCTATGTGAAAACTACCAGTGGCAGCATCATCACTGTAGTACCCAAATCATTAGCTACCT TGGGAGGCAAGATAATTAGCAGTAATATTGTTTCTGGAACGACTACCAAAATCACTACAATCCCAATGAC TTCCAAGCCCAATGTGATTGTTGTACAAAAGACTACAGGAAAAGGAACGACCATTCAAGGCCTCCCTGGC AAAAACGTTGTCACAACCTTGCTAAATGCTGGAGGAGAAAAGACTCTTCAGACAGTGCCAGCTGGAGCCA AGCCAGCTATAATTACTGCTACAAGACCCATCACCAAAATGATTGTAACACAGCCCAAAGGAATAGGTTC CGCAGTACAGCCAGCAGCTAAAATCATTCCAACAAAAATTGTGTATGGACAACAAGGGAAAACACAGGTT CTAATTAAACCCAAACCAGTGACATTTCAAGCTACAGTTGTCAGTGAACAAACAAGGCAGCTGGTAACAG AAACATTACAGCAAGCATCCAGGGTAGCAGATGCCAGTAATTCATCTGCTCAGGAAGGAAAAGAGGAGCC ACAGGGTTACACAGACAGTAGTTCTTCTTCCACAGAGTCCTCCCAGAGTTCTCAAGATTCCCAGCCTGTA GTTCATGTAATTGCTTCCCGGCGTCAGGATTGGTCAGAACATGAGATTGCAATGGAGACTAGCCCCACCA TAATATATCAGGATGTATCCAGTGAATCACAATCAGCCACTTCAACAATCAAAGCTCTGTTAGAACTCCA ACAGACAACAGTAAAGGAAAAATTGGAATCTAAACCAAGACAACCCACTATTGACTTGAGTCAAATGGCA GTACCTATTCAGATGACCCAGGAAAAGAGACATTCTCCTGAGAGTCCATCCATTGCTGTGGTAGAGTCAG AACTAGTTGCTGAATACATCACCACGGTCAGCCATCGCTCCCAGCCCCAACAGCCTTCCCAGCCCCAGCG GACCCTGCTTCAACATGTGGCTCAGTCACAGACTGCGACGCAGACTTCGGTGGTAGTAAAGTCCATCCCA GCGTCTTCCCCTGGAGCAATCACCCACATCATGCAGCAGGCATTAAGCAGTCACACCGCTTTTACGAAAC ACAGTGAGGAACTTGGAACTGAGGAGGGTGAGGTTGAAGAGATGGACACATTGGATCCTCAGACAGGTCT GTTTTACCGCTCTGCCCTGACACAATCGCAGTCAACCAAACAGCAGAAACTTAGCCAGCCCCAGCTGGAA CAGACTCAGCTGCAAGTGAAAACTCTGCAGTGCTTCCAGACCAAACAGAAGCAGACCATCCACCTGCAGG CAGACCAACTCCAGCACAAACTCACACAGATGCCCCAGCTCTCTATCAGGCATCAGAAACTGAATCCTCT CCAGCAGGAACAAGCACAGCCCAAGCCAGATGCACAGCACACACAGCATACTGTGGTGGCCAAAGACAGG CAGCTTCCTACCTTAATGGCACAGCCCCCGCAAACTGTAGTACAGGTGCTTGCCGTGAAAACCACACAGC AGCTTCCTAAACTGCAGCAAGCTCCGAACCAACCAAAAATCTACGTGCAACCCCAAACTCCTCAGAGCCA GATGGCACTCCCCTCTTCAGAGAAACAGCCTGCAAGCCAGGTGGAGCAGCCAATTATAACTCAAGGATCC TCTGTTACAAAGATAACATTTGAGGGGCGCCAGCCTCCCACAGTTACAAAGATAACTGGTGGCAGTTCTG TTCCTAAGCTGACATCACCAGTTACAAGCATATCTCCCATTCAGGCCTCTGAGAAGACAGCAGTGTCAGA CATTTTGCAAATGTCTTTGATGGAAGCTCAGATTGATACAAATGTAGAACATATGGTAGTGGATCCCCCA AAGAAGGCTCTAGCCACCAATGTGCTCACTGGTGAGGCAGGAGCTCTACCCTCCACCCATGTGGTGGTGG CAGGGATGACGAAATGTAGAGAGTCCTGTTCAAGTCCATCTGCTGTTGGCCCTCCCCTAACGACCAGAAA AATCGAAGCAGCAGGAGTGCCTACGACAGGCCAGTTCATGCGTATTCAGAATGTAGGCCAAAAGAAAGCT GAAGAGAGCCCAACAGAAATTATCATCCAGGCCATTCCCCAGTATGCTATTCCTTGTCACTCCAGCTCCA ATGTGGTGGTGGAGCCCAGTGGGCTTCTAGAGTTGAACAACTTCACCAGTCAGCAGCTAGATGACGACGA GACAGCCATGGAGCAGGACATAGACAGCAGCACGGAGGACGGGACTGAGCCCAGCCCCTCTCAGAGCGCT GTAGAACGGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211868 representing NM_172280
Red=Cloning site Green=Tags(s) MPVVWPTLLDLSRDECKRILRKLELEAYAGVISALRAQGDLTKEKKDLLGELSKVLSISTERHRAEVRRA VNDERLTTIAHKMNLSLYLGERPSYSMSGPNSSSEWSIEGRRLVPLMPRLVPQTAFTVTANAVANAAVQH NASLPVPAETASKDGVSCSDEDEKPRKRRRTNSSSSSPVVLKEVPKAVVPVSKTITVPVSGSPKMSNIMQ SIANSLPPHMSPVKITFTKPSTQTTNTTTQKVIIVTTSPSSTFVPNILSKSHNYAAVTKLVPTSVIASTT QKPPVVITASQASLVTSSSNGNSSSTSSPISSTVAVTTVVSSTPSVVMSTVAQGVSTSAIKVASTRLPSP KSLVSGPTQILAQFPKQHQQSPKQQLQQVQQQTQQPVAQPSSVSQQQQPQQSALPPGIKPTIQIKQESGV KIITQQVQPSKILPKPVTATLPTSSNSPIMVVSSNGAIMTTKLVTTPTGTQATYTRPTVSPSLGRVATTP GAATYVKTTSGSIITVVPKSLATLGGKIISSNIVSGTTTKITTIPMTSKPNVIVVQKTTGKGTTIQGLPG KNVVTTLLNAGGEKTLQTVPAGAKPAIITATRPITKMIVTQPKGIGSAVQPAAKIIPTKIVYGQQGKTQV LIKPKPVTFQATVVSEQTRQLVTETLQQASRVADASNSSAQEGKEEPQGYTDSSSSSTESSQSSQDSQPV VHVIASRRQDWSEHEIAMETSPTIIYQDVSSESQSATSTIKALLELQQTTVKEKLESKPRQPTIDLSQMA VPIQMTQEKRHSPESPSIAVVESELVAEYITTVSHRSQPQQPSQPQRTLLQHVAQSQTATQTSVVVKSIP ASSPGAITHIMQQALSSHTAFTKHSEELGTEEGEVEEMDTLDPQTGLFYRSALTQSQSTKQQKLSQPQLE QTQLQVKTLQCFQTKQKQTIHLQADQLQHKLTQMPQLSIRHQKLNPLQQEQAQPKPDAQHTQHTVVAKDR QLPTLMAQPPQTVVQVLAVKTTQQLPKLQQAPNQPKIYVQPQTPQSQMALPSSEKQPASQVEQPIITQGS SVTKITFEGRQPPTVTKITGGSSVPKLTSPVTSISPIQASEKTAVSDILQMSLMEAQIDTNVEHMVVDPP KKALATNVLTGEAGALPSTHVVVAGMTKCRESCSSPSAVGPPLTTRKIEAAGVPTTGQFMRIQNVGQKKA EESPTEIIIQAIPQYAIPCHSSSNVVVEPSGLLELNNFTSQQLDDDETAMEQDIDSSTEDGTEPSPSQSA VERS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_172280 |
ORF Size | 3792 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172280.2, NP_758484.2 |
RefSeq Size | 4031 bp |
RefSeq ORF | 3795 bp |
Locus ID | 233545 |
UniProt ID | Q8BMB0 |
Cytogenetics | 7 E1 |
MW | 135.7 kDa |
Gene Summary | Regulator which is able to repress transcription, possibly via its interaction with a multiprotein chromatin remodeling complex that modifies the chromatin. Its interaction with BRCA2 suggests that it may play a central role in the DNA repair function of BRCA2 (By similarity). As part of a H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224104 | 2210018M11Rik (untagged) - Mouse RIKEN cDNA 2210018M11 gene (2210018M11Rik), (10ug) |
USD 1,211.00 |
|
MG211868 | 2210018M11Rik (tGFP-tagged) - Mouse RIKEN cDNA 2210018M11 gene (2210018M11Rik) |
USD 1,300.00 |
|
MR211868L3 | Lenti ORF clone of 2210018M11Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2210018M11 gene (2210018M11Rik) |
USD 1,400.00 |
|
MR211868L4 | Lenti ORF clone of 2210018M11Rik (mGFP-tagged) - Mouse RIKEN cDNA 2210018M11 gene (2210018M11Rik) |
USD 1,400.00 |
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