Zmynd8 (NM_027230) Mouse Tagged ORF Clone

CAT#: MR211857

Zmynd8 (Myc-DDK-tagged) - Mouse zinc finger, MYND-type containing 8 (Zmynd8)

Product Images

Circular map for MR211857

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zmynd8
• Synonyms: 1110013E22Rik; 2010005I16Rik; 3632413B07Rik; AI316811; AL024039; Prkcbp1; RACK7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211857 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCACAGAGCTTGGCTGAAGAGGAAATAAAAACCGAGCAGGAGGTGGTGGAGGGAATGGATATCT
  CTACTCGCTCCAAAGAACCTAAGGAAGATCCTGTCTCTACAGAGAAAACGGCCCCGAAACGGAGGTTCCC
  CAGCCCTCCACATTCCTCCAATGGCCATTCGCCCCAAGACTCATCCACGAGCCCCATTAAAAAGAAAAAG
  AAACCCGGCTTACTCAACAGTAGCAATAAGGAACAGTCAGAGCTAAGACATGGTCCGTTTTACTATATGA
  AGCAGCCACTCACCACAGACCCTGTTGATGTTGTACCGCAGGACGGACGGAATGACTTCTATTGCTGGGT
  TTGTCACCGGGAAGGACAAGTCCTTTGCTGTGAGCTCTGTCCCCGGGTTTATCACGCTAAGTGTCTGAGA
  CTGACATCGGAGCCAGAGGGGGACTGGTTTTGTCCTGAATGTGAGAAGATTACAGTAGCAGAATGCATCG
  AGACGCAGAGCAAAGCCATGACCATGCTGACCATTGAACAACTGTCCTACCTGCTCAAGTTTGCCATTCA
  GAAAATGAAGCAGCCAGGGACGGATGCATTCCAGAAGCCTGTTCCATTGGAGCAACACCCTGACTATGCA
  GAATATATTTTCCACCCCATGGACCTTTGTACATTGGAAAAGAATGCAAAAAAGAAGATGTACGGCTGCA
  CAGAAGCCTTCCTGGCCGATGCCAAGTGGATCCTGCACAACTGCATTATTTTTAATGGGGGAAATCACAA
  GTTGACGCAAATAGCAAAAGTCGTCATCAAAATCTGTGAGCACGAGATGAATGAAATCGAAGTCTGTCCA
  GAATGTTATCTTGCAGCTTGCCAAAAACGAGACAACTGGTTCTGTGAGCCCTGTAGCAATCCGCACCCTT
  TGGTCTGGGCAAAACTGAAAGGATTTCCATTCTGGCCAGCGAAAGCTCTGAGGGACAAAGACGGGCAGGT
  TGACGCCCGTTTCTTTGGACAACATGACAGAGCCTGGGTTCCAGTCAATAATTGCTACCTCATGTCTAAA
  GAAATCCCCTTTTCTGTGAAAAAGACTAAAAGTATCTTCAACAGCGCCATGCAAGAGATGGAAGTTTACG
  TGGAGAACATACGGAGGAAGTTTGGGGTTTTTAATTACTCCCCGTTCAGGACGCCCTACACGCCCAACAA
  CCAGTACCAAATGCTGCTGGATCCCAGCAACCCCAGCGCGGGCACAGCCAAGACAGACAAACAGGAGAAG
  GTGAAGCTTAATTTTGACATGACAGCGTCCCCCAAGATCCTTCTGAGCAAGCCCCTTCTGAGCGGGGGTG
  CCGGCCGCAGGATCTCCCTGTCCGACATGCCTCGCTCCCCCACCAGTACGAACTCTTCCGTGCACACGGG
  CTCCGATGTGGAGCAGGACCCCGAGAAGAAGGCCCCGTCCAGCCACTTCAGCGCAAGCGAGGAGTCCATG
  GACTTCCTTGATAAGAGCACAGCTTCTCCAGCCTCCACCAAGACGGGGCAAGCCGGGAGCTTGTCTGGCA
  GCCCAAAGCCTTTCTCTCCGCAAGCGCCGACACCCATCATGACAAAACCCGACAAGACTTCCACCTCCAC
  CACCGGGAGCATCCTGAACCTGAACTTGGATCGAAGCAAGGCCGAGATGGACCTGAAGGAGCTGAGCGAG
  TCGGTCCAGCAGCAGTCAGCCCCCGTCCCTCTCATCTCTCCCAAGCGGCAGATTCGAAGCCGGTTCCAGC
  TCAACCTGGACAAGACCATAGAGAGTTGCAAAGCACAGCTAGGCATAAATGAGATCTCAGAGGATGTTTA
  TACAGCCGTGGAGCACAGCGATTCCGAGGACTCCGAAAAGTCGGAGAGCAGCGACAGCGAGTACGTCAGC
  GATGAGGAACAGAAGCCCAAGAATGAGCCCGAGGACCCCGAGGACAAAGAGGGGAGTCGGGTGGACAAAG
  AGGCCCCTGCCATCAAAAGGAAGCCCAAACCCACAAACCAGGTAGAGGTCAAAGAGGAAGCGAAGAGCAA
  CTCTCCTGTCAGCGAGAAGCCGGACCCCACACCCGCCAAGGACAAGGCCAGCCCAGAGCCTGAGAAGGAC
  TTTGTAGAGAAAGCAAAGCCATCACCTCATCCCACAAAGGACAAACTGAAAGGAAAGGATGAAACGGATT
  CTCCCACAGTGCACTTGGGCTTGGATTCGGACTCGGAGAGCGAACTTGTCATAGACTTAGGAGAGGATCC
  TTCTGGGAGGGAGGGTCGAAAAAACAAGAAAGATCCCAAGGTGCCGTCGCCTAAGCAAGACGCTATAGGT
  AAACCGCCACCGTCGTCCACTTCGGCGGGCAACCAGTCTCCCCCAGAGACACCGGTACTCACCCGCTCAG
  CCACCCAAGCACCCGCGGCTGGGGTCACCGTGGCCGCCGCCACCACCAGCACGATGTCTACCGTCACAGT
  CACGGCACCGGCCACCGCCGTCACGGGAAGCCCGGTGAAGAAGCAGAGGCCGCTCTTACCGAAGGAGACT
  GTCCCAGCTGTGCAGCGGGTCGTGTGGAACGCATCAAGTAAGTTTCAAACGTCCTCCCAAAAGTGGCACA
  TGCAGAAGATACAGCGCCAGCAGCAGCAGCAGCAACAACAACAGCAGAGCCAACAGCAGAGCCAGCAGCA
  GCAGCCTCAGTCTTCCCAGGGGACGAGATATCAGACCAGACAGGCTGTGAAAGCTGTCCAGCAGAAGGAG
  GTCACCCAGAGCCCATCCACGTCCACCATCACGCTGGTGACCAGCACACAGCCGGCAGCCCTGGTCAGCA
  GTTCGGGCTCAGCAAGCACCCTGGCGTCTGCAATCAATGCCGACCTTCCCATTGCCACCGCCTCGGCCGA
  CGTGGCCGCAGACATTGCCAAGTACACCAGCAAAATGATGGATGCCATAAAGGGGACGATGACAGAAATC
  TACAATGACCTCTCCAAGAACACCACTGGGAGCACAATAGCTGAGATTCGAAGGCTGAGGATTGAGATTG
  AGAAACTGCAGTGGCTGCACCAGCAGGAGCTCGCTGAGATGAAGCACAACCTGGAGTTGACCATGGCCGA
  GATGCGGCAGAGCCTGGAACAGGAGCGGGATCGGCTCATTGCCGAGGTGAAAAAGCAACTGGAGCTGGAG
  AAGCAGCAGGCGGTGGACGAGACCAAGAAGAAGCAGTGGTGTGCCAACTGCAAGAAGGAGGCCATTTTCT
  ACTGCTGCTGGAACACCAGCTACTGTGACTACCCCTGTCAGCAGGCCCACTGGCCCGAGCACATGAAGTC
  CTGTACCCAGTCGGCGACTGCCCCTCAGCAGGAAGCAGATGCCGAGGCAAGCACAGAAACAGGAAATAAG
  TCATCGCAGGGCAACTCCTCCAACACACAGTCAGCACCTTCAGAACCGGCCAGCGCCCCCAAAGAGAAAG
  AGGCGCCAGCGGAGAAGAGCAAGGACAGTAGTAACTCGACCCTGGATCTTTCCGGCTCCAGAGAGACGCC
  CTCCTCTATGCTCTTAGGCTCCAATCAAAGCTCTGTTAGCAAGAGGTGTGACAAGCAGCCTGCCTATACC
  CCAACCACTACAGACCACCAGCCGCACCCCAACTACCCAGCCCAGAAGTACCATTCCCGGAGCAGCAAGG
  CAGGTTTGTGGAGCAGCAGCGAGGAGAAGCGAGCGTCATCCCGCTCTGAGCACAGTGGAGGGACCAGCAC
  GAAGAACCTCATGCCCAAAGAGTCCCGGGAGTCTCGGCTAGATGCCTTCTGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211857 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHPQSLAEEEIKTEQEVVEGMDISTRSKEPKEDPVSTEKTAPKRRFPSPPHSSNGHSPQDSSTSPIKKKK
  KPGLLNSSNKEQSELRHGPFYYMKQPLTTDPVDVVPQDGRNDFYCWVCHREGQVLCCELCPRVYHAKCLR
  LTSEPEGDWFCPECEKITVAECIETQSKAMTMLTIEQLSYLLKFAIQKMKQPGTDAFQKPVPLEQHPDYA
  EYIFHPMDLCTLEKNAKKKMYGCTEAFLADAKWILHNCIIFNGGNHKLTQIAKVVIKICEHEMNEIEVCP
  ECYLAACQKRDNWFCEPCSNPHPLVWAKLKGFPFWPAKALRDKDGQVDARFFGQHDRAWVPVNNCYLMSK
  EIPFSVKKTKSIFNSAMQEMEVYVENIRRKFGVFNYSPFRTPYTPNNQYQMLLDPSNPSAGTAKTDKQEK
  VKLNFDMTASPKILLSKPLLSGGAGRRISLSDMPRSPTSTNSSVHTGSDVEQDPEKKAPSSHFSASEESM
  DFLDKSTASPASTKTGQAGSLSGSPKPFSPQAPTPIMTKPDKTSTSTTGSILNLNLDRSKAEMDLKELSE
  SVQQQSAPVPLISPKRQIRSRFQLNLDKTIESCKAQLGINEISEDVYTAVEHSDSEDSEKSESSDSEYVS
  DEEQKPKNEPEDPEDKEGSRVDKEAPAIKRKPKPTNQVEVKEEAKSNSPVSEKPDPTPAKDKASPEPEKD
  FVEKAKPSPHPTKDKLKGKDETDSPTVHLGLDSDSESELVIDLGEDPSGREGRKNKKDPKVPSPKQDAIG
  KPPPSSTSAGNQSPPETPVLTRSATQAPAAGVTVAAATTSTMSTVTVTAPATAVTGSPVKKQRPLLPKET
  VPAVQRVVWNASSKFQTSSQKWHMQKIQRQQQQQQQQQQSQQQSQQQQPQSSQGTRYQTRQAVKAVQQKE
  VTQSPSTSTITLVTSTQPAALVSSSGSASTLASAINADLPIATASADVAADIAKYTSKMMDAIKGTMTEI
  YNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELAEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELE
  KQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEASTETGNK
  SSQGNSSNTQSAPSEPASAPKEKEAPAEKSKDSSNSTLDLSGSRETPSSMLLGSNQSSVSKRCDKQPAYT
  PTTTDHQPHPNYPAQKYHSRSSKAGLWSSSEEKRASSRSEHSGGTSTKNLMPKESRESRLDAFWD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027230
• ORF Size: 3768 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027230.2
• RefSeq Size: 5423 bp
• RefSeq ORF: 3768 bp
• Locus ID: 228880
• Cytogenetics: 2 H3
• MW: 139.2 kDa