Ddb1 (NM_015735) Mouse Tagged ORF Clone
CAT#: MR211698
- TrueORF®
Ddb1 (Myc-DDK-tagged) - Mouse damage specific DNA binding protein 1 (Ddb1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_015735" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ddb1 |
Synonyms | 127kDa; AA408517; p127-Ddb1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211698 representing NM_015735
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTACAACTACGTCGTAACGGCGCAGAAGCCTACTGCAGTGAACGGCTGTGTGACCGGACACTTTA CTTCAGCGGAAGATCTGAACCTGTTGATTGCTAAAAACACAAGATTAGAGATCTATGTGGTCACTGCGGA GGGTCTTCGGCCAGTCAAAGAGGTGGGAATGTACGGGAAGATTGCAGTCATGGAGCTCTTCAGGCCCAAG GGGGAGAGCAAAGACCTGCTGTTCATCTTGACTGCTAAATACAATGCCTGTATCTTGGAGTATAAGCAGA GTGGCGAAAGCATTGACATCATTACAAGAGCCCATGGCAATGTCCAGGACCGCATTGGTCGCCCCTCGGA GACTGGCATCATTGGCATCATTGACCCTGAGTGCCGTATGATTGGCCTTCGACTCTACGATGGCCTTTTC AAGGTCATTCCACTAGACCGAGACAATAAAGAGCTCAAGGCCTTTAACATCCGCCTAGAGGAACTGCACG TCATTGATGTCAAGTTCCTGTATGGTTGTCAAGCACCTACCATTTGCTTTGTCTACCAGGATCCTCAGGG GCGGCATGTGAAAACCTATGAGGTGTCTCTTCGAGAGAAGGAGTTCAACAAGGGCCCTTGGAAACAGGAG AATGTAGAAGCCGAAGCTTCAATGGTGATAGCAGTCCCAGAGCCTTTTGGAGGTGCCATCATCATTGGAC AGGAATCAATCACCTATCATAACGGTGATAAATACCTGGCAATTGCTCCTCCTATCATTAAGCAAAGCAC TATTGTGTGCCATAACCGGGTGGATCCTAATGGCTCGCGGTACCTTCTGGGAGATATGGAGGGACGGCTT TTCATGCTGCTCTTAGAGAAGGAGGAGCAGATGGATGGCACTGTCACCCTCAAGGACCTTCGAGTGGAAC TCCTCGGAGAGACCTCCATTGCTGAGTGCCTAACTTATCTTGATAATGGTGTTGTATTTGTCGGGTCTCG CCTAGGTGACTCCCAGCTCGTAAAGCTCAATGTCGACAGCAACGAACAAGGCTCCTATGTAGTGGCCATG GAAACCTTTACCAATTTAGGACCCATTGTGGATATGTGTGTGGTGGACCTGGAGAGGCAGGGACAGGGTC AGCTGGTCACTTGCTCTGGAGCTTTCAAGGAAGGTTCCTTGCGGATCATCCGGAACGGCATTGGAATCCA TGAGCATGCCAGCATTGACTTACCAGGCATCAAAGGTTTATGGCCTCTGCGCTCTGACCCCGGTCGGGAG ACGGATGACACTTTGGTGCTCTCTTTTGTGGGCCAGACAAGAGTTCTCATGCTAAACGGAGAGGAAGTGG AAGAGACAGAACTGATGGGCTTTGTGGACGATCAGCAGACTTTCTTTTGTGGCAACGTGGCTCATCAGCA ACTTATCCAGATCACCTCAGCATCTGTGAGGTTGGTGTCTCAAGAGCCCAAAGCTTTGGTCAGCGAGTGG AAAGAGCCTCAGGGCAAGAACATCAGTGTGGCCTCCTGTAACAGCAGCCAGGTGGTTGTTGCCGTGGGAA GGGCACTATATTACCTTCAGATCCACCCTCAGGAGCTCCGGCAAATCAGCCACACAGAGATGGAACATGA AGTGGCATGCTTGGATATCACCCCATTAGGGGACAGCAATGGCCTCTCCCCACTTTGTGCCATTGGACTT TGGACAGACATCTCAGCTCGTATCCTGAAGCTCCCATCCTTTGAACTACTACACAAGGAGATGCTGGGTG GAGAGATCATTCCTCGATCCATCCTGATGACCACTTTTGAGAGTAGCCACTACCTCCTTTGTGCCTTGGG AGATGGGGCTCTTTTCTACTTTGGGCTCAACATCGAGACAGGTTTACTGAGTGACCGTAAAAAGGTGACT TTGGGCACCCAGCCCACGGTGTTGAGGACATTCCGTTCTCTTTCTACCACCAACGTCTTTGCTTGCTCTG ACCGGCCCACTGTCATCTATAGCAGCAATCACAAGTTGGTCTTCTCCAATGTCAACCTCAAGGAGGTGAA CTATATGTGTCCTCTCAACTCAGATGGCTATCCTGACAGTCTGGCATTGGCCAATAACAGCACTCTCACC ATTGGCACCATCGATGAGATCCAGAAGCTCCATATTCGCACAGTTCCCCTCTATGAGTCTCCCAGGAAGA TCTGCTATCAGGAAGTGTCTCAGTGCTTTGGGGTCCTTTCCAGCCGCATTGAAGTCCAAGATAGCAGTGG AGGCACTACTGCTTTGAGGCCCAGTGCCAGCACCCAGGCCCTGTCCAGCAGCGTCAGCTCCAGCAAACTT TTCTCCAGCAGTACGGCTCCTCACGAGACCTCCTTTGGAGAAGAAGTGGAGGTGCACAACCTCCTCATCA TTGACCAGCACACCTTTGAAGTGCTTCACGCCCATCAGTTTCTACAAAACGAGTATGCCCTCAGCCTGGT CTCCTGCAAGTTGGGTAAAGACCCCAACACTTACTTCATTGTGGGCACAGCCATGGTATATCCTGAAGAG GCAGAGCCCAAGCAGGGTCGTATTGTGGTCTTCCAGTATTCAGATGGAAAACTACAGACTGTGGCTGAAA AGGAAGTAAAAGGAGCTGTGTACTCTATGGTGGAGTTTAATGGGAAGCTGCTTGCCAGCATCAATAGCAC GGTGCGGCTCTATGAGTGGACCACAGAAAAGGAGCTTCGCACTGAGTGTAATCACTACAACAACATCATG GCCCTGTACCTGAAGACCAAGGGCGACTTCATCCTGGTGGGCGACCTCATGCGCTCAGTGCTGCTGCTTG CCTACAAGCCTATGGAAGGAAACTTTGAAGAGATTGCCCGAGACTTTAATCCCAACTGGATGAGTGCAGT GGAGATCTTGGATGATGACAATTTTCTGGGAGCTGAAAATGCCTTTAACTTGTTTGTGTGTCAGAAGGAC AGTGCTGCCACCACTGATGAGGAGCGGCAGCACCTACAGGAGGTTGGTCTCTTCCACCTGGGCGAGTTTG TCAATGTGTTCTGCCATGGCTCCCTAGTTATGCAGAATCTGGGCGAGGCTTCTACCCCTACACAGGGCTC GGTGCTCTTTGGCACAGTCAACGGCATGATAGGGCTGGTGACCTCGCTGTCAGAGAGCTGGTATAACCTC CTGTTGGACATGCAGAACCGACTCAATAAGGTCATCAAAAGCGTGGGCAAGATTGAGCACTCCTTCTGGA GATCCTTTCACACTGAGCGAAAGACAGAACCAGCCACAGGCTTCATCGATGGTGATCTGATTGAAAGTTT CCTAGATATCAGCCGCCCTAAGATGCAGGAGGTTGTGGCAAACTTGCAGTATGATGATGGCAGTGGTATG AAGCGGGAGGCAACTGCAGATGACCTCATCAAAGTCGTGGAGGAGCTAACTCGGATCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211698 representing NM_015735
Red=Cloning site Green=Tags(s) MSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPK GESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLF KVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQE NVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRL FMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAM ETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPGRE TDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEW KEPQGKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGL WTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVT LGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLT IGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDSSGGTTALRPSASTQALSSSVSSSKL FSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEE AEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIM ALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKD SAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGEASTPTQGSVLFGTVNGMIGLVTSLSESWYNL LLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGM KREATADDLIKVVEELTRIH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015735 |
ORF Size | 3420 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015735.2 |
RefSeq Size | 4171 bp |
RefSeq ORF | 3423 bp |
Locus ID | 13194 |
UniProt ID | Q3U1J4 |
Cytogenetics | 19 6.66 cM |
MW | 127.3 kDa |
Gene Summary | Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2 (By similarity). The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1 (PubMed:26431207). DDB1-mediated CRY1 degradation promotes FOXO1 protein stability and FOXO1-mediated gluconeogenesis in the liver (PubMed:28790135).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223704 | Ddb1 (untagged) - Mouse damage specific DNA binding protein 1 (Ddb1), (10ug) |
USD 1,092.00 |
|
MG211698 | Ddb1 (tGFP-tagged) - Mouse damage specific DNA binding protein 1 (Ddb1) |
USD 1,192.00 |
|
MR211698L3 | Lenti ORF clone of Ddb1 (Myc-DDK-tagged) - Mouse damage specific DNA binding protein 1 (Ddb1) |
USD 1,292.00 |
|
MR211698L4 | Lenti ORF clone of Ddb1 (mGFP-tagged) - Mouse damage specific DNA binding protein 1 (Ddb1) |
USD 1,292.00 |
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