Atp1a3 (NM_144921) Mouse Tagged ORF Clone

CAT#: MR211546

Atp1a3 (Myc-DDK-tagged) - Mouse ATPase, Na+/K+ transporting, alpha 3 polypeptide (Atp1a3)

Product Images

Circular map for MR211546

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp1a3
• Synonyms: Atpa-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211546 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACAAAAAAGATGACAAGAGCTCGCCCAAGAAGAGCAAGGCCAAAGAGCGCCGGGACCTGGATG
  ACCTCAAGAAGGAAGTGGCTATGACAGAGCACAAGATGTCAGTAGAAGAGGTCTGCCGGAAATACAATAC
  TGACTGCGTGCAGGGTCTGACACACAGTAAAGCCCAGGAGATCCTAGCCCGGGATGGGCCTAACGCCCTC
  ACACCACCGCCCACCACCCCAGAATGGGTCAAGTTCTGCCGGCAGCTGTTTGGTGGCTTCTCTATCCTGC
  TGTGGATCGGGGCAATCCTTTGCTTCCTGGCCTATGGCATCCAGGCAGGGACGGAGGATGACCCTTCCGG
  TGACAATCTGTACCTGGGCATAGTGCTGGCCGCAGTCGTGATCATCACCGGCTGCTTCTCCTACTACCAA
  GAAGCCAAGAGTTCTAAGATCATGGAGTCCTTCAAGAACATGGTCCCCCAGCAAGCCCTTGTGATCCGGG
  AAGGTGAAAAGATGCAGGTGAATGCGGAGGAGGTGGTGGTCGGGGACCTGGTGGAGATCAAGGGTGGTGA
  CCGGGTGCCAGCTGACCTGCGCATCATCTCGGCCCATGGCTGCAAGGTGGACAACTCCTCCCTGACTGGC
  GAATCTGAGCCTCAGACCCGCTCCCCGGACTGCACACACGACAACCCCCTGGAGACTCGGAACATCACCT
  TCTTTTCCACCAACTGCGTGGAAGGCACCGCTCGGGGTGTGGTGGTAGCCACAGGTGACCGCACCGTCAT
  GGGCCGCATTGCCACCCTGGCCTCGGGCTTGGAGGTGGGCAAGACGCCCATCGCCATTGAGATTGAGCAT
  TTCATCCAGCTCATTACGGGCGTGGCCGTGTTCCTGGGCGTGTCCTTCTTCATCCTCTCTCTCATTCTGG
  GTTACACCTGGCTCGAGGCAGTCATCTTCCTCATCGGCATCATTGTGGCCAATGTCCCAGAGGGGCTGCT
  GGCTACTGTCACGGTGTGTCTGACGCTGACCGCCAAGCGCATGGCTCGCAAGAACTGCCTGGTGAAGAAC
  CTGGAGGCGGTGGAGACGCTGGGCTCCACGTCCACCATCTGCTCGGACAAGACCGGCACTCTCACCCAGA
  ACCGCATGACCGTCGCCCACATGTGGTTTGACAACCAGATCCACGAGGCAGACACCACAGAGGATCAGTC
  AGGGACCTCTTTCGACAAGAGCTCACACACCTGGGTGGCCCTGTCCCACATCGCCGGGCTCTGCAACCGG
  GCCGTCTTCAAGGGCGGGCAGGACAACATCCCAGTACTCAAGAGGGACGTGGCCGGTGATGCCTCCGAGT
  CTGCCCTGCTTAAGTGCATTGAGCTGTCCTCGGGTTCCGTAAAGCTGATGCGAGAACGGAACAAGAAAGT
  GGCTGAGATTCCGTTCAACTCGACCAACAAATACCAGCTATCCATCCATGAGACTGAGGATCCCAATGAC
  AACCGGTACCTGCTAGTGATGAAGGGCGCCCCCGAACGCATTCTGGACCGCTGTGCCACCATCCTCCTGC
  AGGGCAAGGAGCAGCCTCTGGATGAGGAGATGAAGGAGGCCTTCCAGAATGCCTACCTGGAGCTTGGTGG
  CCTGGGCGAGCGTGTGCTGGGTTTCTGCCATTACTACCTGCCTGAGGAACAGTTCCCCAAGGGCTTTGCC
  TTTGACTGTGATGACGTGAACTTCACCACAGACAACCTTTGCTTTGTGGGTCTCATGTCCATGATTGACC
  CTCCCCGGGCAGCCGTCCCTGACGCTGTGGGCAAATGCCGAAGCGCAGGCATCAAGGTCATCATGGTCAC
  CGGCGATCACCCCATCACTGCCAAGGCCATTGCCAAGGGTGTGGGTATCATCTCTGAGGGTAACGAGACT
  GTCGAAGACATCGCTGCCCGGCTCAACATCCCTGTCAGCCAGGTGAACCCCAGGGATGCCAAAGCCTGTG
  TGATTCACGGCACCGACCTCAAGGACTTCACCTCGGAGCAGATTGACGAGATTCTGCAGAACCACACCGA
  GATCGTCTTTGCCCGAACCTCCCCTCAGCAGAAGCTCATCATCGTGGAGGGCTGTCAGAGACAGGGAGCA
  ATTGTGGCTGTGACTGGCGATGGTGTGAATGACTCCCCTGCTCTGAAGAAGGCTGACATCGGGGTGGCCA
  TGGGCATTGCTGGCTCTGATGTCTCTAAGCAGGCTGCCGACATGATTCTGCTGGATGACAACTTTGCTTC
  CATTGTCACTGGTGTGGAGGAAGGCCGCCTGATCTTTGACAACCTGAAGAAATCCATCGCCTACACTCTG
  ACTAGCAACATCCCTGAGATCACACCCTTCCTGCTCTTCATCATGGCTAACATCCCACTGCCTCTTGGCA
  CCATCACCATCCTCTGCATTGACCTGGGTACCGACATGGTCCCTGCAATCTCCCTGGCCTACGAGGCTGC
  CGAGAGCGACATCATGAAGAGGCAGCCCAGGAACCCACGCACAGACAAACTGGTCAACGAAAGGCTCATC
  AGCATGGCCTACGGGCAGATTGGGATGATCCAGGCCCTCGGTGGTTTCTTCTCCTACTTTGTCATCCTGG
  CAGAAAATGGCTTCTTGCCCGGAAACCTGGTGGGCATCCGGCTCAACTGGGATGATCGCACTGTCAATGA
  CCTAGAAGACAGTTATGGGCAGCAGTGGACTTATGAGCAGAGGAAGGTGGTAGAGTTCACATGCCACACA
  GCCTTCTTTGTGAGTATCGTGGTGGTCCAGTGGGCTGACCTGATCATCTGCAAGACCAGGAGGAACTCCG
  TCTTCCAGCAGGGCATGAAGAATAAGATCTTGATCTTCGGCTTGTTTGAGGAGACGGCCCTCGCTGCCTT
  CCTGTCCTACTGCCCAGGCATGGATGTGGCCCTTCGCATGTACCCTCTCAAGCCCAGCTGGTGGTTCTGT
  GCCTTCCCCTACAGTTTCCTCATCTTCGTCTATGATGAGATTCGCAAACTCATCCTGCGCAGGAACCCCG
  GGGGTGAGGGCCGCATGGCCAGGGGTAAAGGCTGGGAGGGGAAGCAGAGGGTGAGCACTCCCTGTGCCTC
  TCAGATGTGTCCGTTCTTCTTTCTCTTCCCTCTCTCCCCGCCTTGCCTTTCACTCTCTTTCCAAGTGCGA
  CTGTGTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211546 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDKKDDKSSPKKSKAKERRDLDDLKKEVAMTEHKMSVEEVCRKYNTDCVQGLTHSKAQEILARDGPNAL
  TPPPTTPEWVKFCRQLFGGFSILLWIGAILCFLAYGIQAGTEDDPSGDNLYLGIVLAAVVIITGCFSYYQ
  EAKSSKIMESFKNMVPQQALVIREGEKMQVNAEEVVVGDLVEIKGGDRVPADLRIISAHGCKVDNSSLTG
  ESEPQTRSPDCTHDNPLETRNITFFSTNCVEGTARGVVVATGDRTVMGRIATLASGLEVGKTPIAIEIEH
  FIQLITGVAVFLGVSFFILSLILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKN
  LEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGTSFDKSSHTWVALSHIAGLCNR
  AVFKGGQDNIPVLKRDVAGDASESALLKCIELSSGSVKLMRERNKKVAEIPFNSTNKYQLSIHETEDPND
  NRYLLVMKGAPERILDRCATILLQGKEQPLDEEMKEAFQNAYLELGGLGERVLGFCHYYLPEEQFPKGFA
  FDCDDVNFTTDNLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNET
  VEDIAARLNIPVSQVNPRDAKACVIHGTDLKDFTSEQIDEILQNHTEIVFARTSPQQKLIIVEGCQRQGA
  IVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTL
  TSNIPEITPFLLFIMANIPLPLGTITILCIDLGTDMVPAISLAYEAAESDIMKRQPRNPRTDKLVNERLI
  SMAYGQIGMIQALGGFFSYFVILAENGFLPGNLVGIRLNWDDRTVNDLEDSYGQQWTYEQRKVVEFTCHT
  AFFVSIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMDVALRMYPLKPSWWFC
  AFPYSFLIFVYDEIRKLILRRNPGGEGRMARGKGWEGKQRVSTPCASQMCPFFFLFPLSPPCLSLSFQVR
  LCV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144921
• ORF Size: 3162 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144921.1, NP_659170.1
• RefSeq Size: 4043 bp
• RefSeq ORF: 3162 bp
• Locus ID: 232975
• Cytogenetics: 7 13.73 cM
• MW: 116 kDa
• Gene Summary: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity).[UniProtKB/Swiss-Prot Function]