Specc1l (NM_153406) Mouse Tagged ORF Clone

CAT#: MR211480

Specc1l (Myc-DDK-tagged) - Mouse sperm antigen with calponin homology and coiled-coil domains 1-like (Specc1l), transcript variant 2

Product Images

Circular map for MR211480

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Specc1l
• Synonyms: 4930470P14Rik; 4932439K10Rik; 9530057A13Rik; AI317249; Cytsa; mKIAA0376
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211480 representing NM_153406
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAAGCAAACAGGAGTGCTGGCTCAGTGCCTAAGGTGTCTGGGATAAGCAAACCGCAAACAGTAG
  AGAAAAGCAAGCCTGAGAACAGCTCTTCAGCACCCACAGGAGTCAAGCCTGTGAGGCCTGGGGCAGCAGC
  AGCCTTGTCAAAGACCAAGAGCAATGATGACCTTTTAGCTGGAATGGCTGGAGGAGTAAACGTGACTAAT
  GGTATTAAAGCAAAGAAAAGCACCTGTTCATCTGCAGCGCCTTCAGCCCCCGCCCCTGCCATGACCATCT
  CGGAGAACAAGTCCAAGATCAGCACAGGCACTAGTTCCTCAGCCAAACGGAGTACTTCGGCTGGTAATAA
  AGAGTCCAGTTCTACTAGAGAAAGATTACGTGAACGCACCCGGCTAAACCAGAGCAAAAAACTGCCCTCT
  GTAAGTCAGGGAGCTAATGATGTGGCACTGGCTAAACGTTCACGCAGCCGCACTGCTGCAGAGGGTGATA
  TTCGTATGAGCAAGTCTAAGTCGGACAATCAGATCAGTGACAAAGCTGCTTTGGAAGCCAAGGTGAAAGA
  TCTCCTCACACTGGCGAAAACCAAGGATGTGGAAATTTTACATTTGAGAAATGAACTCCGAGACATGCGT
  GCTCAGCTGGGCATTAGTGAGGACCATTGTGAAGGTGAGGACAGGTCTGAGGTGAAGGAAACTATTATTG
  CTCACCAGCCTACTGACGTGGAGTCTACTCTCCTGCAGCTGCAGGAGCAGAATACAGCCATCCGCGAGGA
  GCTCAACCAGCTGAAGAATGAAAACAGAATGCTAAAGGACAGGCTCAATGCTTTGGGCTTTTCCCTAGAG
  CAAAGGCTGGACAATTCTGAAAAACTATTTGGCTATCAGTCCCTGAGCCCAGAAATCACCCCTGGTAACC
  AGAGTGACGGAGGAGGAACTCTGACATCTTCGGTAGAAGGTTCTGCTCCTGGGTCGGTGGAAGATCTCCT
  GAGTCAGGATGAAAATACACTCATGGACCATCAGCACAGTAACTCCATGGACAACCTGGACAGTGAGTGC
  AGCGAGGTGTACCAGCCCCTCACTTCCAGCGATGATGCTCTGGACGCCCCCTCCTCTTCAGAGTCAGAGG
  GCGTGCCAAGCATTGAGCGCTCTCGGAAGGGGAGCAGTGGGAATGCCAGTGAGGTGTCCGTCGCTTGCCT
  GACAGAACGGATTCACCAGATGGAAGAGAACCAGCACAGCACCAGTGAGGAGCTTCAGGCCACTCTACAG
  GAGCTGGCTGATCTCCAGCAGATTACCCAGGAGCTCAACAGTGAGAACGAAAGGCTGGGAGAGGAGAAGG
  TCATTCTGATGGAGTCGCTGTGTCAGCAGAGTGATAAGTTGGAACACTTTGGCCGGCAGATTGAGTATTT
  CCGTTCCCTTCTTGATGAGCATCACATTTCCTATGTCATAGATGAAGACGTAAAGAGCGGGCGCTATATG
  GAACTAGAGCAGCGCTACATGGATTTGGCTGAGAATGCCCGTTTTGAGCGAGAGCAGCTTCTAGGTGTGC
  AGCAGCATTTAAGCAACACTTTGAAGATGGCAGAACAAGATAACAAAGAAGCTCAAGAAATGATAGGTGC
  CCTCAAAGAGCGCAGCCACCACATGGAGCGGATTATTGAGTCTGAGCAGAAGGGCAAAGCGGCCCTGGCA
  GCCACATTGGAGGAGTACAAAGCTACTGTGGCCAGTGACCAGATAGAGATGAATCGCTTGAAGGCCCAGC
  TGGAGAACGAAAAGCAGAAGGTGGCTGAGTTGTATTCTATCCATAACTCTGGAGACAAGTCCGATATCCA
  GGATCTCCTGGAGAGTGTCAGGCTGGACAAGGAAAAGGCGGAGACTTTGGCCAGTAGCCTGCAGGAAGAT
  CTGGCTCATACCCGAAATGATGCCAATCGGTTACAGGACACCATTGCTAAGGTAGAAGATGAATACCGAG
  CTTTCCAGGAAGAGGCCAAGAAGCAGATTGAAGATTTGAATATGACATTAGAAAAACTAAGATCAGAGTT
  GGAGGAAAAGGACACGGAGAGGAGTGACATGAAGGAAACCATCTTTGAACTTGAAGATGAGGTGGAGCAG
  CACCGCGCCGTGAAGCTGCATGACAACCTCATTATCTCTGATCTAGAGAATACAGTTAAGAAACTCCAAG
  ACCAAAAGCATGACATGGAAAGAGAAATCAAGACACTTCACAGGAGACTTCGGGAAGAGTCTGCAGAATG
  GCGGCAATTTCAGGCTGATCTCCAGACTGCAGTGGTCATTGCCAATGATATCAAGTCTGAAGCCCAAGAA
  GAGATCGGTGATCTAAAGCGACGGCTACATGAGGCCCAAGAGAAAAATGAGAAACTCACAAAAGAATTGG
  AGGAAATAAAGTCACGCAAGCAAGAGGAAGAGCGTGGCCGGGTGTATAACTACATGAATGCTGTTGAAAG
  AGATTTGGCAGCCTTGAGGCAAGGGATGGGACTGAGTAGAAGGTCCTCCACTTCCTCAGAACCAACTCCC
  ACAGTAAAAACCCTCATCAAGTCTTTCGACAGTGCATCTCAAGTACCAAACGCTGCTGCAGCTGCGATCC
  CTCGAACACCTCTGAGTCCAAGTCCCATGAAAACCCCTCCTGCAGCTGCCGTTTCTCCCATGCAGAGACA
  TTCCATAAGTGGTCCAATCTCAACCTCCAAGCCTCTGACAGCCTTGTCAGATAAGAGATCAAACTATGGA
  GAGCTTCCTGTACAAGAGCATCTACTAAGAACATCTTCAACCAGCCGGCCCGCCTCTCTCCCAAGAGTCC
  CTGCTATGGAAAGTGCCAAGACCATCTCAGTGTCTCGACGGAGTAGTGAAGAAATGAAACGGGACATCTC
  TGCATCTGAGGGCGCGTCACCAGCCTCTCTCATGGCTATGGGCACCACGTCACCACAGCTCTCCCTCTCT
  TCCTCTCCCACTGCATCTGTCACTCCAAGCACCCGGAGCCGAATAAGGGAAGAAAGGAAAGACCCCCTCT
  CAGCATTGGCAAGAGAATATGGAGGCTCCAAGAGGAACGCCTTGTTGAAGTGGTGTCAGAAGAAAACAGA
  AGGCTATCAGAATATTGACATCACAAACTTCAGCAGCAGCTGGAACGACGGGCTGGCTTTCTGCGCATTG
  CTTCATACCTATCTCCCAGCCCACATACCATATCAAGAACTGAACAGTCAAGATAAGAAAAGGAACTTCA
  CCCTGGCTTTCCAGGCAGCTGAAAGTGTTGGAATCAAATCGACGCTGGACATCAATGAAATGGCGCGTAC
  TGAACGGCCCGACTGGCAGAACGTCATGCTGTATGTTACAGCCATCTACAAGTACTTTGAAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211480 representing NM_153406
  Red=Cloning site Green=Tags(s)
  
  MKKANRSAGSVPKVSGISKPQTVEKSKPENSSSAPTGVKPVRPGAAAALSKTKSNDDLLAGMAGGVNVTN
  GIKAKKSTCSSAAPSAPAPAMTISENKSKISTGTSSSAKRSTSAGNKESSSTRERLRERTRLNQSKKLPS
  VSQGANDVALAKRSRSRTAAEGDIRMSKSKSDNQISDKAALEAKVKDLLTLAKTKDVEILHLRNELRDMR
  AQLGISEDHCEGEDRSEVKETIIAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLE
  QRLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSEC
  SEVYQPLTSSDDALDAPSSSESEGVPSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQ
  ELADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFGRQIEYFRSLLDEHHISYVIDEDVKSGRYM
  ELEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALA
  ATLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQED
  LAHTRNDANRLQDTIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSELEEKDTERSDMKETIFELEDEVEQ
  HRAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQE
  EIGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTP
  TVKTLIKSFDSASQVPNAAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRSNYG
  ELPVQEHLLRTSSTSRPASLPRVPAMESAKTISVSRRSSEEMKRDISASEGASPASLMAMGTTSPQLSLS
  SSPTASVTPSTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCAL
  LHTYLPAHIPYQELNSQDKKRNFTLAFQAAESVGIKSTLDINEMARTERPDWQNVMLYVTAIYKYFET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153406
• ORF Size: 3354 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153406.3, NP_700455.3
• RefSeq Size: 6078 bp
• RefSeq ORF: 3357 bp
• Locus ID: 74392
• UniProt ID: Q2KN98
• Cytogenetics: 10 C1
• MW: 124.9 kDa
• Gene Summary: Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration (By similarity).[UniProtKB/Swiss-Prot Function]