Top3a (NM_009410) Mouse Tagged ORF Clone

CAT#: MR211428

Top3a (Myc-DDK-tagged) - Mouse topoisomerase (DNA) III alpha (Top3a)

Product Images

Circular map for MR211428

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Top3a
• Synonyms: MGC106383
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211428 representing NM_009410
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCTTCCCGGTCACCCTCTTAGCGTTTCAGTGGCACCGACGGCCTGGAGGCCGTGCCCTGTCCCGCG
  CTGCCATGGAAGTGGCCTTCCGAGGAGTGCGAAAAGTTCTCTGCGTGGCGGAGAAAAACGACGCTGCCAA
  GGGGATCGCCGACTTGCTGTCCAACGGGCGTATGAGGCGGAAAGAAGGCCTTTCTAAATTCAACAAGATT
  TATGAATTTGACTATCATCTGTATGGCCAGAATGTTACTATGATAATGACTTCAGTCTCTGGACATTTGC
  TGGCTCATGACTTCCAGATGCAGTTTCGGAAATGGCAGAGCTGCAATCCCCTTGTCCTCTTTGAAGCAGA
  AATTGAAAAGTATTGCCCAGAAAATTTTATAGACATCAAGAAAACTCTAGAACGAGAGACACATCATTGT
  CAGGCCCTGGTGATCTGGACGGACTGTGATAGGGAAGGTGAGAACATCGGCTTTGAGATTATCCACGTAT
  GCAAGGCTGTAAAACCCAATCTACGGGTGCTGAGAGCCCGCTTCTCTGAGATCACGCCACACGCCGTCAG
  AACAGCCTGTGAAAACCTAACTGAGCCTGACCAGAGAGTGAGTGACGCAGTGGATGTGAGGCAAGAGCTG
  GACCTGAGGATCGGAGCTGCCTTCACAAGGTTCCAGACCCTGCGGCTCCAGAGGATCTTCCCTGAGGTGC
  TGGCAGAGCAGCTCATCAGCTACGGCAGTTGCCAGTTCCCAACACTGGGGTTTGTGGTGGAGAGGTTCAA
  GGCCATTCAGGCTTTTGTGCCAGAAGTCTTCCACAAAATTAAAGTAACTCATGACCACAAAGATGGGACC
  GTAGAGTTCAACTGGAAACGATACCGTCTCTTTAACCACACAGCTTGTCTTGTCCTTTATCAGTTGTGCA
  TGGAGGATCCCATGGCGACTGTGGTAGAGGTCAGATCTAAGCCAAAGAGCAAGTGGAGGCCGCAGGCTTT
  GGACACGGTGGAGCTAGAGAAACTGGCATCTCGGAAATTGAGAATAAATGCCAAAGAAACCATGAGGATC
  GCGGAAAAGCTCTATACACAAGGGTACATCAGCTACCCCCGGACAGAAACAAACATCTTCCCCAAAGACT
  TAAACCTGGTTGCATTGGTAGAACAGCAGACCGTGGATCCACACTGGGGGGCTTTTGCTCAGACCATTCT
  AGAGCGAGGTGGCCCTACTCCCCGAAACGGGAGCAAGTCTGATCAAGCTCACCCTCCCATTCACCCCACC
  AAATACACCAGCGGCTTGCAGGGAGATGATCGGCGACTGTACGAGTTCATTGTTCGCCATTTCCTGGCTT
  GCTGCTCTCAGGACGCTCAGGGGCAAGAGACTACCGTAGAGATCGACATTGCCCAAGAGCGCTTTGTGGC
  CCACGGGCTCATCATTCTGGCCCGTAACTACCTAGATGTGTATCCGTATGATCACTGGAGTGACAAGCTC
  CTCCCCGTCTATGAACAAGGCTCCCACTTCCAGCCCAGCACTGTGGAAATGGTGGATGGAGAGACTAGCC
  CACCCCAACTGCTTACCGAAGCTGACCTCATCGCCCTCATGGAGAAACATGGGATCGGTACTGATGCAAC
  TCATGCAGAACACATTGAGACCATCAAAGCCCGGATGTATGTGGGACTCACCTCAGACAAGCGGTTCCTG
  CCGGGGCACCTGGGCATGGGACTTGTAGAAGGTTACGATTCCATGGGTTATGAGATGTCCAAGCCTGACC
  TCCGTGCCGAGCTGGAAGCTGATCTCAAGCTGATCTGTGAGGGCAAGAAGGATAAGTTTCAAGTTCTAAG
  GCAGCAAGTGCAAAAATACAAGCAGGTTTTCATTGAAGCAGTGGCGAAGGCAAAGAAGTTGGATGAGGCT
  TTATCTCAGTACTTAGGAGAAAGAACAGAGATGGCCCAGCAAGAAGAGATCTACCCAGCCATGCCAGAGC
  CTGTTCGGAAGTGCCCACAGTGTAACAAGGATATGGTCCTCAAGACCAAGAAGAGTGGTGGGTTCTACCT
  CAGCTGCATGGGGTTCCCAGAGTGTCGGTCAGCTGTGTGGTTTCCTGACTCGGTGCTGGAGGCCAGCAGG
  GACAACAGTGTGTGTTCTGTTTGTCAGCCACCCCCTGTGTACAGGTTGAAGTTGAAGTTTAAGCGTGGTA
  GCCTTCCCCCAGCTATGCCCCTGGAGTTTGTCGGCTGCATAGGTGGATGTGATGAGACTTTGAAGGAAAT
  CTTCGGCCTGCGATTTCCACGGGCTCTCCCAAGAGCTAGCCAGCCCTCTGGCCACCTGCAGGCCAGCCAG
  GCCCTCAACAGGATGGACAGCAGTCAGCATAACCTGTCTCAACCTCTGGTTAACAGACATACTAGACCTT
  CAAAGACAGTGGCCCAAGCCTTGCTACCACCCACTACTGCTGGTGAAAGCAACTCTGTGACTTGCAATTG
  TGGCCGGGAGGCTGTGCTGCTCACTGTCCGCAAGCAGGGCCCCAACCAGGGCCGGCATTTCTACAAGTGC
  AGTAATGGTGACTGCAACTTCTTCCTGTGGGCTGACAGCAGCCATTCCACTGGAGGAGGGACCCCCACCT
  CTGCATCAGGACCCCCAGGCAGCTCTGTAGGATGCCCATCCAGTGTAGGCAGCCACATGGATGGGTTTGG
  CAGCCTTGGCAGCGACAGTGATGGAGGTACACCCTGCCTGTGCGGGCAGCCTGCTGTCACACGGACTGTT
  CAGAAGGATGGACCCAACAAAGGACGCCAGTTCCACACCTGTGCCAAGCCACGAGAGCAGCAGTGTGGCT
  TCTTTCAGTGGGTGGATGAGAATGTGGCCCCAGGAAGTTTTGCAGCCCCTGCATGGCCAGGAGGCAGAGG
  AAAAGCCCAGAGACCAGAGGCTGCAAGCAAAAGACCAAGAGCTGGTTCCTCAGATGCAGGGTCCACAGTA
  AAAAAGCCTCGGAAATGTAGCCTTTGTCACCAGCCTGGACACACCCGAACCTTTTGTCCTCAGAACAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211428 representing NM_009410
  Red=Cloning site Green=Tags(s)
  
  MIFPVTLLAFQWHRRPGGRALSRAAMEVAFRGVRKVLCVAEKNDAAKGIADLLSNGRMRRKEGLSKFNKI
  YEFDYHLYGQNVTMIMTSVSGHLLAHDFQMQFRKWQSCNPLVLFEAEIEKYCPENFIDIKKTLERETHHC
  QALVIWTDCDREGENIGFEIIHVCKAVKPNLRVLRARFSEITPHAVRTACENLTEPDQRVSDAVDVRQEL
  DLRIGAAFTRFQTLRLQRIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEVFHKIKVTHDHKDGT
  VEFNWKRYRLFNHTACLVLYQLCMEDPMATVVEVRSKPKSKWRPQALDTVELEKLASRKLRINAKETMRI
  AEKLYTQGYISYPRTETNIFPKDLNLVALVEQQTVDPHWGAFAQTILERGGPTPRNGSKSDQAHPPIHPT
  KYTSGLQGDDRRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLIILARNYLDVYPYDHWSDKL
  LPVYEQGSHFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSDKRFL
  PGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKFQVLRQQVQKYKQVFIEAVAKAKKLDEA
  LSQYLGERTEMAQQEEIYPAMPEPVRKCPQCNKDMVLKTKKSGGFYLSCMGFPECRSAVWFPDSVLEASR
  DNSVCSVCQPPPVYRLKLKFKRGSLPPAMPLEFVGCIGGCDETLKEIFGLRFPRALPRASQPSGHLQASQ
  ALNRMDSSQHNLSQPLVNRHTRPSKTVAQALLPPTTAGESNSVTCNCGREAVLLTVRKQGPNQGRHFYKC
  SNGDCNFFLWADSSHSTGGGTPTSASGPPGSSVGCPSSVGSHMDGFGSLGSDSDGGTPCLCGQPAVTRTV
  QKDGPNKGRQFHTCAKPREQQCGFFQWVDENVAPGSFAAPAWPGGRGKAQRPEAASKRPRAGSSDAGSTV
  KKPRKCSLCHQPGHTRTFCPQNR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009410
• ORF Size: 3009 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009410.3
• RefSeq Size: 3740 bp
• RefSeq ORF: 3012 bp
• Locus ID: 21975
• UniProt ID: O70157
• Cytogenetics: 11 B2
• MW: 112.8 kDa
• Gene Summary: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2.[UniProtKB/Swiss-Prot Function]