Prrt3 (NM_172487) Mouse Tagged ORF Clone

CAT#: MR211350

Prrt3 (Myc-DDK-tagged) - Mouse proline-rich transmembrane protein 3 (Prrt3)

Product Images

Circular map for MR211350

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prrt3
• Synonyms: 6330505P20; B230206N24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211350 representing NM_172487
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCAGCCCACAGGCCTGCACATCTCCTCTTCTGCTGTTGCTGCTGCCATGCCTGGGGGCTGGCC
  CTGCCTTGGGCAGAGGCCTTCCCAGGCCCCTGGAGAACTCAGAACCTCACATGATCCCATCAGAGTCACA
  GACCTTTGACCTCTTCTGGGAGAAGCTGAGAAATGAGAGTTCCTGGCACAGCGGTGACCCTCAGGCCCGT
  GCTGAGGGGCCCAAGAAGCCCGCAGACCCTTACCTAGGACCAGCCCTGCATGGGCCCAAGGCAGCCCCTG
  GGGTCCAAGGTGAAAGACTACTAAGAGCTGATGACCTCCAGCTGGCCCGAGCATTCACCTCCCAAGGCTG
  GACAGGACCTCCTGACTCACAGGAGCTTCTGGAGCCTGAGGCACCAGAACCCCACCCTGTGCGAGCACCT
  CGTCTCACTCTTGTCACTACAACTCCCAGCTCCCTACTCTCGGCAGCCATACTTTCCACTGCCTCCCAGA
  AGCCTGGAGGCACAGCAGGGCAGCAGCCAGCTAGGAACGAGGAGCTAATAATGGTCAAAGCCGAGACCCA
  CATCACACAGGCTTCCCCCTGGGACTTCCAGGGCTCTTCCCACACTCCGGTACCCGAGACAGATGCTGTG
  AGGACGCTGGTGTTGGGGAAGCAGGGGGGACATGAACAGGGCTTCCAGGAGGCTGTCCAGGGTCCCCTAC
  TCACCCAGCAGGATCCAGTAGTCCCTGGGGTCGGCTCAACACCCCCAGTTAAAGTGGAATCCACCCCAGA
  GCCTGGAGCCCAGCTCGACCTGGCATTGGTCAGAAGCCTTCCTCTTCCTGAGGGGCTGCCAGCTGAACCC
  CCCAAAACTGGAGCTGGGGATACCTGGGAAGTCAGCTCTCTGGGACCCCAACCAGAACAGACCGATCTCC
  TTGGAGGACAAGATTCCCCAGCACCCCAGCCTATACCACCTTCAGCCTCAGACACTTCTGATGGGCACCT
  AAGACCAGTATCCTCGCTGAATGGAGCCGACCCCATCTCCCCACAACGTGTGAGAGGAGCGATGGAGGCC
  CCAGGAACCCCCAAGTCCTTCATCCCTGACCTTCCAAATTCTGCTCAAGCTGCAAATGGAACAGAGAGCC
  CTGTGAGGGCCCTGCAGCCAGATGAAGCCGAGGATTGGCCGGGTCGCCCACAAAGCCACCCTCCAGCACC
  CCCTGTCCAGGCCCCCTCGACATCTCGGCGTGGTCTCGTTAGAGTCACCACACAGAGAGCTCTGGGCCAG
  CCACTCCCTCCGGAGCCCTCAGCCAGCTCCATAGTCCCCATCCCAGCTTCTAGCCCCCCAGCCAATGCCA
  CCGCACCTCCCCTGCGTTGGGGACCCCTTCGGCGGGTGCTGAGTTTCTCCTGGGAGCTTCATGTGTATGG
  GGTGGGGGTGCTTTTCCTGCTTCCAGCATTATTGGCATTGGTCACAGTGGCAGCCGCCCTAGCGGGACCC
  CGATTAGCACTAGTGGCTGCCGCGCTGGTATTGGTGGCTTCAGGGCTGCGATCTGCCTACATGCTCACGG
  ATCCTTATGGCTCTCAGGCGCGACTGGGAGTACGTGCAGGTCTGGTGCTATACAACTTGCCCTTCCCCTT
  GCTACTCACTGCACTGGCAGCACTCACCCTGCTGGGCTTGGGTGCTGGTCTGCCACAGCCTCTGCAGAAG
  CCGCTGCTGCTGGGAATTGTGGCACCGGTGCATGGCACATGTTTGCTGGCTACAGACCTGTTCTCCACCA
  GCCCTGTGCTCAACCTTCTAACACAAGGCTTATCTTGCGCCTGGGGCGCCTCAGTGGCTCTGGGCACACT
  CTGCCTGTGCCGTCGACGCCTGCTGGAAGGCCCTCGGGGCTGGGATGCCAGTCCAGGGCCCCGGCTGTTG
  GCTGTGGCGGGCTCACTGGGGCTGTTGGCCAGTGGTCTGCAGTTGGCGGCCTCTCTCTGGCTGTACCCAG
  GGCCTGGCCGGGAGGGTCGCTTCTCCTGGGCTTGGTGGGGCGTGCACTTCTGGCTGCGCCTGCTGGAATT
  GACCTGGGCACTGGCCCTGGCGCTAGCTGCCCTGGCTGCCACTCGTCCCAGGCCGCCCACGGAGCACGCT
  TGCTGGGCTAAGCTGCTGCGTCTGGCGTGCCCCGCGCCTACAGGGAAGAGCGAAGTTCCCGAGAGACCCA
  ATAACTGCTATGCGGGGCCCAGTGGCCTGGGCACAGGCGGCCTGGACATCAGCAAAAGTCTCATCCGCAA
  CGCTGCTGGGGAAGCTGGGCTTCCTGTCACTCCTGGTTCTGGACCCTGGGGTTCGGCTGCATCGCTGGGT
  CGCGGTCGTCCAGGTGGCCAGAGGATGTCCCGGGGCAGTGTGGGGCCCGCACCGTCGCTGAGCGAGCTGG
  ATCTGCGGCCACCGTCACCTATCAATTTGAGCCGCAGCATCGACGCCGCGCTCTTCCGGGAGCACTTGGT
  TCGAGAGAGTGTGTTCCAACGCTGTGGTCTCCGTGGCTTGGCCTCCTCGCCGACAGGGGGCGCCCTGCGG
  CCTCGCCGCGGCAGCCAGCCCGATGCCGAGCTCGACGGCGCGGGCACATCATTGCTCCGAGGCCGCTGCC
  GCTCGCTCACCGAGGTGTGCTTGCGCACGTCGCTCCCGCAGCACGTGATGGAGCCGCCCGTCGGCGCGGC
  GGCCGCGGGGACTTCGGGCAGCTCCCTGGACAGTTTCTCCAAGGGCTCGCTGAAGATCAGCTGGAACCCG
  TGGCGTCACGGGCTGTCATCCGTGGACAGCCTGCCCCTAGATGAACTGCCCAGCACCGTGCAGCTGCTGC
  CACCACCTACCCCAGTCCCTGCTCCTGCCCGGGCGGGGGAGCCTCAGGGCGAGGGCCAGTCGCGTTGCAA
  GTCCAGCGAGTCTCACAGCGCCTCTAGTGACACTATAGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211350 representing NM_172487
  Red=Cloning site Green=Tags(s)
  
  MAPSPQACTSPLLLLLLPCLGAGPALGRGLPRPLENSEPHMIPSESQTFDLFWEKLRNESSWHSGDPQAR
  AEGPKKPADPYLGPALHGPKAAPGVQGERLLRADDLQLARAFTSQGWTGPPDSQELLEPEAPEPHPVRAP
  RLTLVTTTPSSLLSAAILSTASQKPGGTAGQQPARNEELIMVKAETHITQASPWDFQGSSHTPVPETDAV
  RTLVLGKQGGHEQGFQEAVQGPLLTQQDPVVPGVGSTPPVKVESTPEPGAQLDLALVRSLPLPEGLPAEP
  PKTGAGDTWEVSSLGPQPEQTDLLGGQDSPAPQPIPPSASDTSDGHLRPVSSLNGADPISPQRVRGAMEA
  PGTPKSFIPDLPNSAQAANGTESPVRALQPDEAEDWPGRPQSHPPAPPVQAPSTSRRGLVRVTTQRALGQ
  PLPPEPSASSIVPIPASSPPANATAPPLRWGPLRRVLSFSWELHVYGVGVLFLLPALLALVTVAAALAGP
  RLALVAAALVLVASGLRSAYMLTDPYGSQARLGVRAGLVLYNLPFPLLLTALAALTLLGLGAGLPQPLQK
  PLLLGIVAPVHGTCLLATDLFSTSPVLNLLTQGLSCAWGASVALGTLCLCRRRLLEGPRGWDASPGPRLL
  AVAGSLGLLASGLQLAASLWLYPGPGREGRFSWAWWGVHFWLRLLELTWALALALAALAATRPRPPTEHA
  CWAKLLRLACPAPTGKSEVPERPNNCYAGPSGLGTGGLDISKSLIRNAAGEAGLPVTPGSGPWGSAASLG
  RGRPGGQRMSRGSVGPAPSLSELDLRPPSPINLSRSIDAALFREHLVRESVFQRCGLRGLASSPTGGALR
  PRRGSQPDAELDGAGTSLLRGRCRSLTEVCLRTSLPQHVMEPPVGAAAAGTSGSSLDSFSKGSLKISWNP
  WRHGLSSVDSLPLDELPSTVQLLPPPTPVPAPARAGEPQGEGQSRCKSSESHSASSDTIEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172487
• ORF Size: 2913 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172487.4, NP_766075.2
• RefSeq Size: 3304 bp
• RefSeq ORF: 2916 bp
• Locus ID: 210673
• UniProt ID: Q6PE13
• Cytogenetics: 6 E3
• MW: 101.7 kDa