Ap1b1 (NM_007454) Mouse Tagged ORF Clone
CAT#: MR211270
- TrueORF®
Ap1b1 (Myc-DDK-tagged) - Mouse adaptor protein complex AP-1, beta 1 subunit (Ap1b1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_007454" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ap1b1 |
Synonyms | Adtb1; b2b1660Clo |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211270 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGACTCAAAATACTTCACCACAACCAAGAAAGGGGAGATCTTTGAGCTGAAGGCAGAGCTTAACA GTGACAAGAAGGAGAAGAAGAAGGAGGCAGTGAAGAAAGTGATTGCATCAATGACTGTGGGCAAAGATGT CAGTGCTCTCTTCCCTGATGTGGTGAACTGCATGCAGACAGACAACCTGGAGCTAAAGAAGCTGGTGTAC CTGTACTTGATGAACTATGCCAAGAGTCAGCCTGACATGGCCATTATGGCTGTCAACACCTTTGTGAAGG ACTGTGAGGACCCCAATCCTCTCATCCGGGCCCTGGCTGTGAGGACTATGGGCTGCATCCGAGTTGACAA GATTACAGAGTATTTGTGTGAACCACTCCGGAAGTGCCTGAAGGATGAAGACCCATATGTGCGCAAGACA GCGGCTGTGTGTGTGGCCAAGCTCCATGACATCAATGCCCAGCTGGTAGAAGACCAGGGCTTCCTGGACA CGCTTAAAGACCTCATCTCAGACTCTAACCCCATGGTGGTAGCGAATGCAGTGGCTGCCCTGTCAGAGAT CGCTGAGTCTCACCCCAGTAGCAACCTGCTTGACTTGAACCCACAGTCTATCAACAAGCTGCTGACAGCC CTTAATGAGTGCACTGAATGGGGCCAGATCTTCATTCTGGACTGTCTGGCCAACTACATGCCCAAGGATG ACCGTGAGGCCCAGAGCATCTGTGAGCGGGTCACTCCTAGACTCTCCCATGCCAACTCTGCTGTGGTGCT GTCTGCGGTGAAGGTGCTGATGAAGTTCATGGAGATGCTGTCCAAGGACCTGGACTACTATGCCACACTG CTCAAGAAGCTGGCCCCTCCCCCTGTCACACTGCTGTCCGCAGAGCCAGAGCTGCAGTATGTGGCCCTGC GCAACATCAATCTCATTGTACAGAAAAGGCCAGAGATCTTGAAGCATGAAATGAAGGTTTTCTTTGTGAA GTACAATGACCCCATCTACGTGAAGCTGGAGAAACTGGACATTATGATCCGCCTGGCCTCCCAGGCCAAC ATTGCCCAGGTATTGGCAGAACTCAAAGAGTATGCTACAGAAGTAGACGTGGATTTCGTACGGAAGGCAG TGCGTGCCATTGGCCGCTGTGCCATCAAGGTGGAGCAATCTGCAGAGCGCTGTGTGAGCACATTGCTTGA TCTCATCCAGACCAAGGTCAACTATGTGGTTCAGGAAGCCATTGTGGTCATTAAGGACATCTTCCGCAAG TACCCCAACAAGTATGAGAGTGTGATAGCTACACTGTGTGAGAATCTGGACTCTCTGGATGAGCCTGAGG CCCGAGCCGCTATGATCTGGATAGTGGGCGAGTATGCCGAGCGGATTGACAATGCAGACGAGCTGCTCGA GAGCTTCCTGGAAGGTTTCCATGATGAGAGCACTCAGGTCCAGCTACAGCTACTGACAGCCATTGTGAAA CTCTTCCTGAAGAAGCCAACAGAGACTCAGGAGCTGGTGCAGCAGGTCCTCAGTTTGGCCACTCAGGACT CAGACAATCCAGACCTTCGGGACCGTGGCTACATCTACTGGCGCTTGCTCTCCACGGACCCTGTAGCTGC TAAAGAGGTGGTACTGGCTGAGAAGCCCCTCATCTCAGAGGAGACAGACCTTATTGAGCCTACACTTCTG GATGAACTCATCTGCTACATTGGCACGCTGGCCTCTGTCTACCACAAGCCTCCCAACGCCTTTGTGGAGG GTGGCCGGGGAGTGGTACATAAGAGCCTGCCACCCCGCACTGCCTCGAGTGAGAGCACAGAGAGCCCTGA GACAGCTCCTGCTGGAGCACCTGCTGGTGATCAGCCTGATGTCATTCCTGCCCAGGGTGACCTGTTAGGT GACCTCCTCAACCTGGACCTTGGGCCCCCAGTGAGTGGCCCACCCTTAGCTGCCTCCTCCGTGCAAATGG GAGCTGTGGATCTTCTTGGTGGTGGCCTCGACAGCCTGATTGGAGATAGCAACTTTGGGGCACCTTCAGC ATCAGTGGCGGCAGCACCAGCACCAGCCAGACTTGGAGCACCCATCAGCAGTGGCTTAAGTGACCTCTTT GACCTGACCAGTGGTGTGGGCACCCTTTCAGGATCCTATGTAGCTCCCAAAGCAGTCTGGCTCCCTGCCA TGAAGGCCAAGGGACTGGAGATCTCTGGCACCTTCACCCGGCAGGCTGGCTCCATCTCTATGGACCTGCA GCTGACTAACAAGGCCCTGCAGGTCATGACAGATTTTGCTATCCAGTTCAATCGTAACAGCTTTGGCTTG GCCCCTGCTGCACCTCTTCAGGTGCACGTACCGCTCAGCCCCAACCAGACCGTGGAGATCTCCCTGCCTC TTAACACAGTGGGCTCAGTCTTGAAGATGGAGCCTCTAAACAATCTTCAGGTGGCTGTGAAGAACAACAT TGACGTCTTCTACTTCAGCACTTTGTACCCACTGCATGTCCTCTTCGTGGAGGATGGGAAGATGGACCGG CAGATGTTCCTGGCCACATGGAAGGACATTGCCAACGAGAACGAAGCCCAGTTCCAGATCAGAGACTGCC CGCTTAACACAGAGGCTGCGAGCAACAAGCTACAGAGCAGTAACATTTTCACTGTCGCCAAGAGGAACGT GGAGGGCCAGGATATGCTCTACCAATCTCTGAAGTTGACCAATGGCATCTGGGTGCTGGCGGAGCTGCGG ATCCAGCCAGGCAACCCCAGCTTCACGCTGTCCCTGAAGTGTCGAGCACCGGAGGTGTCCCAGCATGTAT ACCAGGCCTACGAGACTATCCTCAAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211270 protein sequence
Red=Cloning site Green=Tags(s) MTDSKYFTTTKKGEIFELKAELNSDKKEKKKEAVKKVIASMTVGKDVSALFPDVVNCMQTDNLELKKLVY LYLMNYAKSQPDMAIMAVNTFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKT AAVCVAKLHDINAQLVEDQGFLDTLKDLISDSNPMVVANAVAALSEIAESHPSSNLLDLNPQSINKLLTA LNECTEWGQIFILDCLANYMPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFMEMLSKDLDYYATL LKKLAPPPVTLLSAEPELQYVALRNINLIVQKRPEILKHEMKVFFVKYNDPIYVKLEKLDIMIRLASQAN IAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIKDIFRK YPNKYESVIATLCENLDSLDEPEARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLQLLTAIVK LFLKKPTETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVAAKEVVLAEKPLISEETDLIEPTLL DELICYIGTLASVYHKPPNAFVEGGRGVVHKSLPPRTASSESTESPETAPAGAPAGDQPDVIPAQGDLLG DLLNLDLGPPVSGPPLAASSVQMGAVDLLGGGLDSLIGDSNFGAPSASVAAAPAPARLGAPISSGLSDLF DLTSGVGTLSGSYVAPKAVWLPAMKAKGLEISGTFTRQAGSISMDLQLTNKALQVMTDFAIQFNRNSFGL APAAPLQVHVPLSPNQTVEISLPLNTVGSVLKMEPLNNLQVAVKNNIDVFYFSTLYPLHVLFVEDGKMDR QMFLATWKDIANENEAQFQIRDCPLNTEAASNKLQSSNIFTVAKRNVEGQDMLYQSLKLTNGIWVLAELR IQPGNPSFTLSLKCRAPEVSQHVYQAYETILKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007454 |
ORF Size | 2832 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007454.1, NM_007454.2, NM_007454.3, NP_031480.2 |
RefSeq Size | 4109 bp |
RefSeq ORF | 2832 bp |
Locus ID | 11764 |
UniProt ID | O35643 |
Cytogenetics | 11 A1 |
MW | 103.9 kDa |
Gene Summary | Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200521 | Ap1b1 (untagged) - Mouse adaptor protein complex AP-1, beta 1 subunit (Ap1b1), (10ug) |
USD 864.00 |
|
MG211270 | Ap1b1 (tGFP-tagged) - Mouse adaptor protein complex AP-1, beta 1 subunit (Ap1b1) |
USD 1,064.00 |
|
MR211270L3 | Lenti ORF clone of Ap1b1 (Myc-DDK-tagged) - Mouse adaptor protein complex AP-1, beta 1 subunit (Ap1b1) |
USD 1,164.00 |
|
MR211270L4 | Lenti ORF clone of Ap1b1 (mGFP-tagged) - Mouse adaptor protein complex AP-1, beta 1 subunit (Ap1b1) |
USD 1,164.00 |
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