Dgkq (NM_199011) Mouse Tagged ORF Clone
CAT#: MR211245
- TrueORF®
Dgkq (Myc-DDK-tagged) - Mouse diacylglycerol kinase, theta (Dgkq)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_199011" in other vectors (4)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Dgkq |
Synonyms | 110kDa; DAGK; Dagk4; DAGK7; Dgkd |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211245 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCAGCCGAGCCCGGTGCCCGGACCTGGCCGGGCAGTGGTTCCCCACGTCTTGGCAGCCCGG CCGGCAGTCCAGTGCTGGGCATCTCGGGCCGCACACGCCCGGGGTCAGGGCCGGAGCGGACTAGCAGAGC TATTGGCTCCGCAGCACCTGGCCACAGCTTTCGCAAGGTGACACTCACCAAGCCTACCTTCTGCCACCTC TGCTCGGACTTCATCTGGGGACTGGCTGGCTTCCTGTGCGATGTCTGCAACTTCATGTCCCATGAGAAGT GCCTGAAGCAGGTGAAGACCCCGTGCACAAGCATTGCACCAAGCCTCGTCCGGGTGCCTGTAGCCCACTG CTTTGGTTCCCTTGGTCTCTACAAGCGCAAGTTCTGTGTGGTCTGCCGCAAGAGCCTGGAGGTACCCGCA TTCCGCTGTGAAGTGTGTGAGCTGCACGTTCACCCCGACTGTGTGCCCTTCGCCTGCAGCGACTGTCGTC AGTGCCACCAGGATGGACAGCAGGATTATGACACGTATCACCACCACTGGAGGGAGGGGAACCTGCCTTC TGGTGCACGATGTGAGGTCTGTAGGAAGACTTGTGGTTCCTCAGATGTGCTGGCTGGTGTACGCTGCGAG TGGTGTGGTGTACAGGCTCACTCAGTGTGCTCCACAGCACTTGCGCCTGAGTGTACATTTGGACGTCTAC GCTCCATGGTACTGCCTCCTTCGTGTGTGCGCCTGTTGTCCCGAAACTTCAGCAAGATGCACTGTTTCCG AATCCCTGAGACCATGGTCCTGGAGCTTGGTGATGGGGATGATGGCGTAGACGGGAGTGCTGCTATAGGC ACAGGCAGAGAGGTACTGACAGCTACAGAGTCCACCAAACAGACCCTGAAGATCTTTGATGGCAACGACT CCATGAGGAAAAATCAGTTTCGTCTGGTTACAGTTTCCCGCCTGGCTCGGAATGAGGAAGTGATGGAGGC AGCACTTCGGGCCTACTATATTAGCGAGGACCCTAAGGACTTCCAGCTGCAGGCACTGCCCCTGTCTGGC AATGCCCAGGCTCTGGGGAAGGCTGGGACCACTGAGGAGGAGGCTAGTAAAGGCTCTTGTCCCCGGGATT CCGTGCCTGAGGCCTGGGTCATCAGGTCTTTGCCTCGTACCCAAGAGATCCTGAAGATCTACCCTGGCTG GCTCAAGGTAGGTGTGGCCTACGTGTCCATCCGTGTGAACTCCCAGAGTACAGCACGGTCTGTGGTTCAA GAGGTTCTCCCACTGTTTGGACAACAGGTTGAGGATAAGGAGAGATTCCAGCTGATTGAGGTGCTCATGA GCAGCAGACAAGTCCAACGGACCGTGCTGGCAGATGAAGAACCTCTGCTAGACCGACTCTGGGACATCCG ACAGACTTCTGTGCGCCAGGTGAGCCAGACGCGGTTCTACGTGGCCGAGACCAGGGCCACGGCCCCACGT GTCTCCCTGTTCGTGGGTGGCCTGCCACCTGGCTTGTCCCCCCAGGATTACAGCAACCTGCTGCATGAGG CCATGGCCACCAAAGCTGCTGTGGTGTCTGTGAGTCACGTCTACTCCTTACAAGGTGCAGTAATTCTGGA CGTCACCTGCTTCGCGGAGGCTGAGCGGCTATACATGCTGGCCAGGGACACAGCAGTGCATGGCCGGCCA CTGACTGCATTAGTGCTTCCAGATGTGCTGCACACGAAGCTGCCTCCTGACTGCTGCCCTCTCCTCGTGT TTGTGAACCCCAAGAGTGGGGGTCTCAAGGGACGAGAACTGCTCTGCAGTTTCCGGAAGCTGCTGAATCC GCACCAGGTCTTTGAGCTAACCAACGGGGGCCCTCTTCCTGGGTTCCACCTTTTCTCCCAGGTGCCCAGT TTTCGGGTACTGGTGTGTGGTGGAGATGGCACCGTTGGCTGGGTGCTCGCTGCCCTGGAGGAGACAAGGC GCCATCTGGCCTGTCCAGAGCCATCTGTGGCCATCTTGCCCCTGGGTACAGGGAATGACCTTGGCCGGGT CCTTCGTTGGGGGGCAGGCTATAGTGGTGAGGACCCATTTTCTGTGCTGGTGTCGGTGGATGAGGCTGAT GCTGTGCTCATGGATCGATGGACAATCCTGCTGGATGCTCATGAAATTGATAGTACAGAGAACAATGTGG TAGAAACAGAGCCCCCCAAGATTGTTCAGATGAATAACTACTGTGGCATTGGTATTGATGCGGAGCTCAG CCTGGACTTCCACCAGGCACGTGAAGAGGAGCCTGGCAAATTCACAAGCAGGTTCCACAACAAGGGCGTG TATGTGCGGGTCGGGCTGCAGAAGATCAGCCACTCTCGAAGCCTACACAAGGAGATCCGGCTGCAGGTGG AGCAGCAGGAGGTGGAGCTACCCAGCATTGAGGGTCTCATCTTCATTAACATCCCCAGCTGGGGCTCAGG GGCCGACTTGTGGGGCTCTGACAACGACTCAAGGTTTGAGAAGCCACGCATAGACGACGGGCTGTTGGAG GTGGTGGGTGTGACAGGTGTCGTGCACATGGGCCAGGTACAAGGTGGGCTACGCTCTGGAATCCGCATTG CCCAGGGCTCCTACTTCCGTGTCACACTCCTCAAGGCTACTCCAGTGCAGGTGGATGGTGAGCCCTGGGT TCAGGCCCCAGGTCACATGATCATCTCTGCTACTGCACCTAAGGTTCACATGCTGAGAAAGGCTAAGCAG AAGCCCAGGAAGGCTGGCGCCAACAGGGATACCCGAGTGGACACCTTGCCTGCTCCTGAGGGCAATCCTT TA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211245 protein sequence
Red=Cloning site Green=Tags(s) MAAAAEPGARTWPGSGSPRLGSPAGSPVLGISGRTRPGSGPERTSRAIGSAAPGHSFRKVTLTKPTFCHL CSDFIWGLAGFLCDVCNFMSHEKCLKQVKTPCTSIAPSLVRVPVAHCFGSLGLYKRKFCVVCRKSLEVPA FRCEVCELHVHPDCVPFACSDCRQCHQDGQQDYDTYHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCE WCGVQAHSVCSTALAPECTFGRLRSMVLPPSCVRLLSRNFSKMHCFRIPETMVLELGDGDDGVDGSAAIG TGREVLTATESTKQTLKIFDGNDSMRKNQFRLVTVSRLARNEEVMEAALRAYYISEDPKDFQLQALPLSG NAQALGKAGTTEEEASKGSCPRDSVPEAWVIRSLPRTQEILKIYPGWLKVGVAYVSIRVNSQSTARSVVQ EVLPLFGQQVEDKERFQLIEVLMSSRQVQRTVLADEEPLLDRLWDIRQTSVRQVSQTRFYVAETRATAPR VSLFVGGLPPGLSPQDYSNLLHEAMATKAAVVSVSHVYSLQGAVILDVTCFAEAERLYMLARDTAVHGRP LTALVLPDVLHTKLPPDCCPLLVFVNPKSGGLKGRELLCSFRKLLNPHQVFELTNGGPLPGFHLFSQVPS FRVLVCGGDGTVGWVLAALEETRRHLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLVSVDEAD AVLMDRWTILLDAHEIDSTENNVVETEPPKIVQMNNYCGIGIDAELSLDFHQAREEEPGKFTSRFHNKGV YVRVGLQKISHSRSLHKEIRLQVEQQEVELPSIEGLIFINIPSWGSGADLWGSDNDSRFEKPRIDDGLLE VVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISATAPKVHMLRKAKQ KPRKAGANRDTRVDTLPAPEGNPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_199011 |
ORF Size | 2805 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199011.2 |
RefSeq Size | 4612 bp |
RefSeq ORF | 2805 bp |
Locus ID | 110524 |
UniProt ID | Q6P5E8 |
Cytogenetics | 5 53.24 cM |
MW | 102.3 kDa |
Gene Summary | Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP-induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222705 | Dgkq (untagged) - Mouse diacylglycerol kinase, theta (Dgkq), (10ug) |
USD 942.00 |
|
MG211245 | Dgkq (tGFP-tagged) - Mouse diacylglycerol kinase, theta (Dgkq) |
USD 1,056.00 |
|
MR211245L3 | Lenti ORF clone of Dgkq (Myc-DDK-tagged) - Mouse diacylglycerol kinase, theta (Dgkq) |
USD 1,156.00 |
|
MR211245L4 | Lenti ORF clone of Dgkq (mGFP-tagged) - Mouse diacylglycerol kinase, theta (Dgkq) |
USD 1,156.00 |
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