Eps15 (NM_007943) Mouse Tagged ORF Clone

CAT#: MR211092

Eps15 (Myc-DDK-tagged) - Mouse epidermal growth factor receptor pathway substrate 15 (Eps15), transcript variant 1

Product Images

Circular map for MR211092

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eps15
• Synonyms: 2410112D09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211092 representing NM_007943
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGCAGCCCAGCTCTCCCTGACACAGTTGTCAAGTGGGAATCCTGTATATGAAAAATACTACA
  GACAGGTTGAGGCAGGCAATACTGGAAGGGTGTTGGCGTTAGATGCTGCTGCATTCCTGAAAAAGTCAGG
  GCTTCCAGACTTGATTCTTGGAAAGATTTGGGATTTAGCTGACACAGATGGCAAAGGTGTCCTGAGCAAA
  CAAGAATTCTTTGTTGCTTTACGGCTTGTGGCATGTGCTCAGAATGGACTGGAAGTTTCACTGAGTAGCC
  TAAGTCTGGCTGTTCCTCCACCAAGATTTCATGACTCCAGCAGTCCGTTGCTAACCAGTGGGCCCTCAGT
  TGCTGAGCTCCCGTGGGCTGTAAAGTCTGAAGATAAAGCCAAATATGATGCAATTTTTGACAGTTTAAGC
  CCAGTGGATGGATTTTTGTCTGGTGATAAAGTGAAACCAGTGTTGCTCAACTCTAAGTTACCTGTGGAAA
  TCCTTGGAAGAGTTTGGGAGTTGAGTGATATTGACCACGATGGAAAGCTGGACAGAGATGAGTTTGCAGT
  TGCCATGTTTTTGGTATACTGTGCACTGGAGAAAGAACCTGTGCCAATGTCCTTGCCTCCAGCCTTGGTG
  CCACCTTCTAAGAGAAAAACGTGGGTTGTATCCCCTGCAGAAAAAGCTAAATATGATGAAATTTTTCTGA
  AAACTGATAAGGATATGGATGGATATGTGTCTGGACTGGAGGTCCGTGAAACCTTCCTGAAAACAGGTTT
  ACCTTCTGCCTTGCTAGCCCACATTTGGTCACTATGTGACACAAAGGGCTGTGGGAAGCTTTCAAAAGAC
  CAGTTTGCCTTGGCTTTTCACTTAATCAATCAGAAGTTAATAAAAGGCATTGACCCTCCTCATAGTCTCA
  CTCCTGAGATGATTCCACCATCAGACAGATCCAGTTTACAAAAGAACATCACAGGATCAAGTCCTGTTGC
  AGATTTTTCTGCTATTAAGGAACTAGATACCCTTAACAATGAAATAGTTGACCTGCAGAGGGAAAAGAAC
  AATGTGGAGCAGGACCTTAAAGAGAAGGAAGACACAGTTAAGCAGAGGACCAGTGAGGTTCAGGATCTTC
  AAGATGAAGTTCAAAGGGAGAGTATTAATCTACAAAAACTGCAGGCCCAGAAGCAGCAGGTGCAGGAGCT
  CCTGGGTGAACTGGATGAGCAGAAAGCCCAGCTGGAGGAGCAGCTCCAGGAAGTCAGGAAAAAGTGTGCT
  GAGGAGGCCCAGCTGATTTCTTCCCTGAAAGCAGAAATAACTAGTCAAGAATCTCAGATCTCCAGTTATG
  AGGAAGAACTGTTGAAAGCTAGAGAAGAACTAAGTCGCCTACAACAAGAAACAGCACAATTGGAAGAAAG
  TGTGGAGTCAGGGAAGGCTCAGCTGGAACCTCTTCAGCAGCACCTACAAGAGTCACAACAGGAAATCAGC
  TCAATGCAAATGAGATTGGAAATGAAAGATCTGGAAACTGATAATAACCAATCAAATTGGAGCAGTAGCC
  CACAAAGCGTTCTTGTTAATGGTGCTACAGATTACTGTAGCCTCAGCACCAGCAGCAGTGAAACAGCCAA
  CTTCAACGAACATGCTGAAGGCCAAAACAACCTAGAGTCTGAACCCACACACCAGGAGTCCTCAGTAAGA
  AGTAGTCCTGAAATCGCACCTTCTGATGTGACTGATGAAAGTGAGGCTGTGACTGTGGCTGGTAATGAGA
  AAGTTACTCCGAGATTTGACGATGACAAGCACTCAAAAGAGGAAGATCCATTTAATGTAGAATCAAGTTC
  ACTGACAGATGCAGTTGCAGATACAAACTTGGATTTTTTCCAGTCTGATCCTTTTGTTGGCAGTGATCCT
  TTCAAGGATGATCCTTTTGGAAAAATTGATCCATTTGGTGGTGACCCTTTCAAAGGCTCAGATCCTTTTG
  CGTCTGATTGCTTCTTTAAGCAGACTTCTACTGATCCTTTTACCACTTCAAGTACGGACCCTTTCAGTGC
  ATCCAGCAACAGCAGTAATACATCGGTAGAAACTTGGAAGCACAATGACCCATTTGCTCCTGGTGGAACA
  GTTGTTGCTGCAGCGAGTGATTCAGCCACAGACCCTTTTGCTTCTGTTTTCGGAAATGAATCATTTGGAG
  ATGGATTTGCTGACTTCAGCACATTATCAAAGGTCAACAATGAAGATGCTTTTAATCCTACCATATCAAG
  TTCTACCAGCAGTGTGACCATTGCAAAACCTATGTTAGAGGAAACAGCCAGCAAGAGTGAAGATGTGCCT
  CCAGCACTGCCGCCCAAAGTTGGCACTCCAACAAGACCTTGCCCGCCACCCCCTGGGAAAAGACCCATCA
  ACAAATTGGATTCTTCTGATCCCCTTAAACTGAATGATCCATTTCAGCCTTTCCCAGGCAATGATAGTCC
  CAAAGAAAAAGATCCTGATATGTTTTGTGATCCATTCACTTCTTCTACCACTACCAATAAAGAGGCTGAC
  CCAAGCAATTTTGCTAACTTCAGTGCTTATCCCTCTGAAGAAGATATGATTGAATGGGCAAAAAGGGAAA
  GTGAGCGGGAAGAAGAACAGAGGCTTGCCAGACTAAATCAGCAGGAGCAAGAAGACTTGGAACTGGCCAT
  TGCACTTAGCAAATCTGAGATCTCAGAAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211092 representing NM_007943
  Red=Cloning site Green=Tags(s)
  
  MAAAAQLSLTQLSSGNPVYEKYYRQVEAGNTGRVLALDAAAFLKKSGLPDLILGKIWDLADTDGKGVLSK
  QEFFVALRLVACAQNGLEVSLSSLSLAVPPPRFHDSSSPLLTSGPSVAELPWAVKSEDKAKYDAIFDSLS
  PVDGFLSGDKVKPVLLNSKLPVEILGRVWELSDIDHDGKLDRDEFAVAMFLVYCALEKEPVPMSLPPALV
  PPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGYVSGLEVRETFLKTGLPSALLAHIWSLCDTKGCGKLSKD
  QFALAFHLINQKLIKGIDPPHSLTPEMIPPSDRSSLQKNITGSSPVADFSAIKELDTLNNEIVDLQREKN
  NVEQDLKEKEDTVKQRTSEVQDLQDEVQRESINLQKLQAQKQQVQELLGELDEQKAQLEEQLQEVRKKCA
  EEAQLISSLKAEITSQESQISSYEEELLKAREELSRLQQETAQLEESVESGKAQLEPLQQHLQESQQEIS
  SMQMRLEMKDLETDNNQSNWSSSPQSVLVNGATDYCSLSTSSSETANFNEHAEGQNNLESEPTHQESSVR
  SSPEIAPSDVTDESEAVTVAGNEKVTPRFDDDKHSKEEDPFNVESSSLTDAVADTNLDFFQSDPFVGSDP
  FKDDPFGKIDPFGGDPFKGSDPFASDCFFKQTSTDPFTTSSTDPFSASSNSSNTSVETWKHNDPFAPGGT
  VVAAASDSATDPFASVFGNESFGDGFADFSTLSKVNNEDAFNPTISSSTSSVTIAKPMLEETASKSEDVP
  PALPPKVGTPTRPCPPPPGKRPINKLDSSDPLKLNDPFQPFPGNDSPKEKDPDMFCDPFTSSTTTNKEAD
  PSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007943
• ORF Size: 2691 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007943.3, NP_031969.1
• RefSeq Size: 5193 bp
• RefSeq ORF: 2694 bp
• Locus ID: 13858
• UniProt ID: P42567
• Cytogenetics: 4 C7
• MW: 98.9 kDa
• Gene Summary: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.[UniProtKB/Swiss-Prot Function]